Urinary subclasses of glutathione-s-transferase (α и Ï€) as an indicator of early damages in human kidney tubular cells

Abstract

The aim of this work was to ob tained in for ma tion about the pos si bil ity of dif fer en ti at ing (with a-and p-GST) be tween cel lu lar dam age at dif fer ent lev els of the tu bu lar sys tem in spe cific forms of acute re nal fail ure (ARF) -ischemic vs. ob struc tion. The study fo cused spe cif i cally on dif fer enc ing be tween acute allograft re jec tion and cyclosporineA in duced nephrotoxicity. Pa tients and meth ods. Twenty two pa tients, hos pi tal ized at the Depart ment of Nephrology, Clin i cal cen tre-Skopje, suf fer ing from ARF with the av er age age 48.1±5.6 years were stud ied. Forty five re cip i ents with re nal allograft were fol lowed daily from the time of trans plan ta tion un til dis charge from hos pi tal and then twice weekly for a to tal 3 months. Urine sam ple were ob tained from patients with ARF as an ischemic tu bu lar ne cro sis and acute ob struc tive re nal in suf fi ciency and pa tients with renal trans plan ta tion. The con cen tra tion of a-and p-GST in urine were mea sured fol low ing the method of quan ti ta tive EIA (Biotrin Int.LTD). The se rum sam ples from trans planted pa tient were mea sured for creatinine and CystatinC. RESULTS. Sig nif i cant dif fer ences of ð-GST val ues be tween ischemic ARF and acute re nal ob struc tion were ob served, while they were in sig nif i cant for the val ues of a-GST. There were no sig nif i cant dif fer ences for a-GST val ues of all three sam ples in both groups with ARF. In the cases of acute rejec tion af ter trans plan ta tion, the re lease of a-GST into the urine is lim ited, while that of the p-GST isoenzyme is ex ten sive. In con trast to the case of acute re jec tion, in the early stages of CsA in duced nephrotoxicity a signif i cant amount of a-GSTs ex creted into the urine, but no sig nif i cant el e va tion of uri nary p-GST is seen.