Urinary Tract Pathogens with a Special Reference to Mucoid
            <i>Pseudomonas Aeruginosa</i>
            at Aflaj General Hospital

Odugbemi, T; Anandan, N.; Lina, Olivia F.

doi:10.5144/0256-4947.1992.581

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…Some clinical isolates of P. aeruginosa from human urinary tract infections demonstrate a mucoid phenotype (40,41). We asked whether urine or urea would induce the production of alginate in vitro.…”

Section: Resultsmentioning

confidence: 99%

Catheter-Associated Urinary Tract Infection by Pseudomonas aeruginosa Is Mediated by Exopolysaccharide-Independent Biofilms

et al. 2014

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Pseudomonas aeruginosa is an opportunistic human pathogen that is especially adept at forming surface-associated biofilms. P. aeruginosa causes catheter-associated urinary tract infections (CAUTIs) through biofilm formation on the surface of indwelling catheters. P. aeruginosa encodes three extracellular polysaccharides, PEL, PSL, and alginate, and utilizes the PEL and PSL polysaccharides to form biofilms in vitro; however, the requirement of these polysaccharides during in vivo infections is not well understood. Here we show in a murine model of CAUTI that PAO1, a strain harboring pel, psl, and alg genes, and PA14, a strain harboring pel and alg genes, form biofilms on the implanted catheters. To determine the requirement of exopolysaccharide during in vivo biofilm infections, we tested isogenic mutants lacking the pel, psl, and alg operons and showed that PA14 mutants lacking these operons can successfully form biofilms on catheters in the CAUTI model. To determine the host factor(s) that induces the ΔpelD mutant to form biofilm, we tested mouse, human, and artificial urine and show that urine can induce biofilm formation by the PA14 ΔpelD mutant. By testing the major constituents of urine, we show that urea can induce a pel-, psl-, and alg-independent biofilm. These pel-, psl-, and alg-independent biofilms are mediated by the release of extracellular DNA. Treatment of biofilms formed in urea with DNase I reduced the biofilm, indicating that extracellular DNA supports biofilm formation. Our results indicate that the opportunistic pathogen P. aeruginosa utilizes a distinct program to form biofilms that are independent of exopolysaccharides during CAUTI.

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Section: Resultsmentioning

confidence: 99%

Catheter-Associated Urinary Tract Infection by Pseudomonas aeruginosa Is Mediated by Exopolysaccharide-Independent Biofilms

et al. 2014

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“…Multiple studies have documented the ability of P. aeruginosa isolated from multiple infection sites to form biofilms (49,50). P. aeruginosa can cause middle ear infections in cholesteatoma patients (51,52). 83% of otitis media P. aeruginosa isolates are efficient biofilm producers, with substantially higher levels of biomass produced than PAO1.…”

Section: Discussionmentioning

confidence: 99%

Genotypic and Phenotypic Analyses of Two Distinct Sets ofPseudomonas aeruginosaUrinary Tract Isolates

Ebrahim,

Haldenby,

Moore

et al. 2023

Preprint

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Urinary tract infections (UTIs) are associated with a high burden of morbidity, mortality, and cost.Pseudomonas aeruginosaemploys a myriad of virulence factors, including biofilm formation and motility mechanisms, to cause infections including persistent UTIs.P. aeruginosais highly resistant to antibiotics and the World Health Organization has identified it as a pathogen for which novel antimicrobials are urgently required. Genotypic and phenotypic characterization ofP. aeruginosafrom UTIs are underreported. In addition, the rise of antimicrobial resistance (AMR) is a cause for concern, particularly in many countries where surveillance is severely lacking.22P. aeruginosaUTI isolates were sourced from the United Kingdom (UK) and Kuwait. To establish the phenotypes of UK isolates, growth analysis, biofilm formation assays, motility assays, and antibiotic disc diffusion assays were performed. Whole genome sequencing, antimicrobial susceptibility assays, andin silicodetection of AMR-associated genes were conducted on both sets of isolates.In terms of their phenotypic characteristics and genomic composition, the UTI isolates varied. Multiple resistance genes associated with resistance to various classes of antibiotics, such as aminoglycosides, fluoroquinolones, and β-lactams, particularly in isolates from Kuwait. Extreme antibiotic resistance was detected in the isolates obtained from Kuwait, indicating that the country may be an antibiotic resistance hotspot.This study highlights that isolates from UTIs are diverse and can display extremely high resistance. Surveillance in countries such as Kuwait are currently limited and this study suggest the need for greater surveillance.

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Urinary Tract Pathogens with a Special Reference to Mucoid Pseudomonas Aeruginosa at Aflaj General Hospital

Cited by 2 publications

References 3 publications

Catheter-Associated Urinary Tract Infection by Pseudomonas aeruginosa Is Mediated by Exopolysaccharide-Independent Biofilms

Catheter-Associated Urinary Tract Infection by Pseudomonas aeruginosa Is Mediated by Exopolysaccharide-Independent Biofilms

Genotypic and Phenotypic Analyses of Two Distinct Sets ofPseudomonas aeruginosaUrinary Tract Isolates

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