Urinary Trypsin Inhibitor Ameliorates Seawater Immersion-Induced Intestinal Mucosa Injury via Antioxidation, Modulation of NF-<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi mathvariant="bold">κ</mml:mi></mml:mrow></mml:math>B Activity, and Its Related Cytokines in Rats with Open Abdominal Injury

Zhang, Xing Jian; Wang, Ya Li; Song, Zhanqian; Xue, Xiao-Jun; Liu, Qiang; Zhang, Wen Hua; Zheng, Jiawei

doi:10.1155/2014/858237

Cited by 7 publications

(6 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mammalian NF-κB family consists of five members, including RelA (also named p65), RelB, c-Rel, NF-κB1 p50, and NF-κB2 p52, all of which form homo- or hetero-dimers in the cytoplasm. [ 20 ] NF-κB proteins are normally bound to inhibitory molecules of the IκB family of proteins as an inactive complex. Inflammatory cytokines, such as IL-1β and TNFα, can activate the canonical NF-κB pathway, start the phosphorylation and degradation of the IκB protein, particularly IκBα, and cause the onset of NF-κB translocation to the nucleus and target gene transcription.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effect of total saponins from Dioscorea nipponica makino on gouty arthritis based on the NF-κB signal pathway: An In vitro study

Zhou¹,

Liu

Yua³

et al. 2016

Phcog Mag

View full text Add to dashboard Cite

Objective:Dioscorea nipponica Makino is one of the most common used traditional Chinese drugs which are used to treat gouty arthritis (GA). Nuclear factor-κB (NF-κB) pathway plays an important role during this process. In the present study, we investigated the effects of total saponins from D. nipponica Makino (TDN) on NF-κB pathway in interleukin-1β (IL-1β) induced fibroblast-like synoviocytes (FLS).Materials and Methods:FLS were divided into three groups: Normal group, model group, which was given 10 μg/L IL-1β to induce the proliferation, and TDN group (10 μg/L IL-1β +100 μg/L TDN). 1 h, 24 h, 48 h, and 72 h after treating, immune fluorescence method was used to detect the cell location of NF-κB p65. Electrophoretic mobility shift assay was used to detect the activation of NF-κB p65. Western blot method was used to detect the protein expressions of NF-κB p65, IκBα, and p-IκBα.Results:TDN could inhibit the activation and transfer of NF-κB p65. As time went on, the expression of NF-κB p65 in the cytoplasm was decreased while it was increased in the nucleus. The expression of p-IκBα was increased, whereas the expression of IκBα was not changed. TDN could regulate these abnormal expressions.Conclusion:TDN may treat GA by regulating NF-κB signal pathway.SUMMARY TDN could inhibit the transfer of NF-κB p65.TDN could inhibit the activation of NF-κB p65.TDN could inhibit the expression of p-IκBa. Abbreviations used: TDN: Total saponins from Dioscorea nipponica Makino, GA: Gouty arthritis, FLS: Fibroblast-like synoviocytes, IL-1β: Interleukin-1 beta, IF: Immune fluorescence, EMSA: Electrophoretic mobility shift assay, WB: Western blot.

show abstract

Section: Discussionmentioning

confidence: 99%

Therapeutic effect of total saponins from Dioscorea nipponica makino on gouty arthritis based on the NF-κB signal pathway: An In vitro study

Zhou¹,

Liu

Yua³

et al. 2016

Phcog Mag

View full text Add to dashboard Cite

show abstract

“…Previous study in our group has shown that hydrogen gas inhalation attenuates seawater instillation-induced ADLI in rabbits and that the protective effects observed may be related to the activation of the Nuclear factor erythroid 2-related factor 2 (Nrf2) pathway [4]. Additionally, the release of reactive oxygen species and increased cell apoptosis stimulated by seawater instillation were possible mechanisms of lung injury [5,6].…”

Section: Introductionmentioning

confidence: 91%

The Protection Effect of Ulinastatin on Freshwater Instillation-Induced Acute Lung Injury in Rabbits

Su¹,

Huan²,

Wu³

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Drowning is an important cause of accidental death in humans. The main cause of death following drowning is pulmonary oedema or lung injury, eventually leading to acute respiratory distress syndrome. The present study aimed to determine the protective effects of Ulinastatin on freshwater-induced acute drowning lung injury. Methods: Rabbits were randomly divided into a control, freshwater, freshwater + small-dose Ulinastatin, freshwater + medium-dose Ulinastatin, freshwater + large-dose Ulinastatin group. The arterial blood gas analysis was performed before modelling (baseline) and at various time points after freshwater instillation. And then, the wet-to-dry weight ratio lung permeability index were measured to detect the effect of Ulinastatin on lung endothelial permeability. Furthermore, histopathological staining and ELISAs were used to analyse the histological changes and inflammatory cytokines expression resulted from lung injury, respectively. Western blotting and Quantitative real-time polymerase chain reaction were used to measure the protein and mRNA levels of Hypoxia inducible factor-lα (HIF-1α)/ Vascular endothelial growth factor (VEGF) in the lung tissues. Results: By inhibiting the HIF-1α/VEGF pathway, treatment with Ulinastatin at a large dose could markedly attenuate changes in the PaO2/FiO2 (P/F), lung endothelial permeability, histopathology, and the expression of inflammatory cytokines induced by freshwater instillation. Conclusion: Ulinastatin is a potential candidate treatment for freshwater drowning-induced acute lung injury that targets the HIF-1α/VEGF pathway.

show abstract

“…A 6 cm x 6cm gauze was put into 99℃ boiling water for ten seconds, and then was used to prepare 10% of rats °burn of rat [9][10] . Abdominal injury was made by opening the abdomen of the middle of the abdomen, the incision length is 3 cm located under the xiphoid [11] . After the process the rat is stable for about ve minutes, rats in SI group were immersed in 15℃ arti cial seawater for one hour, the waterline is at the level of the xiphoid process,for maintaining its normal breathing.In the Ctl group, except no immersing in seawater, the other treatment methods were the same.…”

Section: Animal Modelsmentioning

confidence: 99%

Effect of seawater immersion on pathophysiological changes in rats combined burns with open abdominal injury

ying

Liu

jifu

2020

Preprint

View full text Add to dashboard Cite

Objective: To observe the effects of seawater immersion on the pathophysiological changes in rats combined burns with open abdominal injury.Method: Eighty-eight male Sprague-Dawley(SD) rats combined burns with open abdominal injuries were randomly divided into the seawater immersion group (SI) and the control group (Ctl).Rats in SI group were immersed in seawater at 15℃ for 1 hour. The changes of respiration, blood pressure, rectal temperature, blood gases, blood electrolyte, liver and kidney function, coagulation function, heart damage and animal survival at different time points in the early stage (within 9 hours after injury) were observed.Result: After immersed in seawater, the body temperature of rats in SI group were significantly decreased, the respiration were remarkably inhibited and the blood pressure were obviously declined as compared to those in Ctl group. The blood pH of rats in SI group were significantly lower than those in Ctl group, showing severe acidosis(P<0.05). The changes of HCO3-, PCO2 and BE also presented the similiar trends(P<0.05). PO2 and PCO2 in SI group were significantly higher than those in Ctl group (P<0.05). However, the SaO2 in SI group were lower than those in Ctl group (P<0.05). Blood sodium and chlorine in SI group were higher than those in Ctl group, showing severe hypernatremia and hyperchloremia(P<0.05). The concentration of potassium and calcium were not obviously changed. The liver and kidney function parameters(AST,ALT,Urea,Crea) in SI group were lower than those in Ctl group 4 hours after injury(P<0.05), while myocardial damage markers (TNT) in SI group were higher than those in Ctl group. There were no significant differences in coagulation function between the two groups. The survival time of the SI group rats were shorter than those in Ctl group and the mortality was higher than those in Ctl group (66.7% vs 41.7%).Conclusion: Low-temperature seawater immersion might aggravate injury, accelerate animal death, induce hypothermia, respiratory depression, acidosis, hypernatremia and hyperchloremia. Immersing in low temperature seawater for 1 hour can cause myocardial damage, but it has protective effects on the liver and kidney. Therefore, more attentions should be paid to the secondary injury caused by seawater immersion in the emergency treatment of the wounded, such as rapidly rewarming, rectifying the acidosis and maintaining the stability of homeostasis of the wounded, so as to lay the foundation for the further definitive treatments.

show abstract

Urinary Trypsin Inhibitor Ameliorates Seawater Immersion-Induced Intestinal Mucosa Injury via Antioxidation, Modulation of NF-κB Activity, and Its Related Cytokines in Rats with Open Abdominal Injury

Cited by 7 publications

References 33 publications

Therapeutic effect of total saponins from Dioscorea nipponica makino on gouty arthritis based on the NF-κB signal pathway: An In vitro study

Therapeutic effect of total saponins from Dioscorea nipponica makino on gouty arthritis based on the NF-κB signal pathway: An In vitro study

The Protection Effect of Ulinastatin on Freshwater Instillation-Induced Acute Lung Injury in Rabbits

Effect of seawater immersion on pathophysiological changes in rats combined burns with open abdominal injury

Contact Info

Product

Resources

About