Urine Cell-Free MicroRNAs in Localized Prostate Cancer Patients

Abstract

Prostate cancer (PCa) is the most common cancer in men. Prostate-specific antigen screening is recommended for the detection of PCa. However, its specificity is limited. Thus, there is a need to find more reliable biomarkers that allow non-invasive screening for early-stage PCa. This study aims to explore urine microRNAs (miRs) as diagnostic biomarkers for PCa. We assessed cell-free miR (cfmiR) profiles of urine and plasma samples from pre- and post-operative PCa patients (n = 11) and normal healthy donors (16 urine and 24 plasma) using HTG EdgeSeq miRNA Whole Transcriptome Assay based on next-generation sequencing. Furthermore, tumor-related miRs were detected in formalin-fixed paraffin-embedded tumor tissues obtained from patients with localized PCa. Specific cfmiRs signatures were found in urine samples of localized PCa patients using differential expression analysis. Forty-two cfmiRs that were detected were common to urine, plasma, and tumor samples. These urine cfmiRs may have potential utility in diagnosing early-stage PCa and complementing or improving currently available PCa screening assays. Future studies may validate the findings.

Combination of Urinary MiR-501 and MiR-335 With Current Clinical Diagnostic Parameters as Potential Predictive Factors of Prostate Biopsy Outcome

“…Whole-genome miRNA profiling studies also described upregulation of miR-335-5p in the tissue of high grade PCa tumors (13) and in exosomes secreted by human PCa cells under hypoxic conditions (14). The only exploratory study describing miR-335-5p as a potential biomarker of prostate cancer is the work by Koh et al, where this miRNA was differentially expressed in urine, plasma, and tissue samples of PCa patients (15).…”

The Vicious Cycle of Melanoma-Microglia Crosstalk: Inter-Melanoma Variations in the Brain-Metastasis-Promoting IL-6/JAK/STAT3 Signaling Pathway

“…MCM prepared from four MBM cell lines or starvation medium were added to microglia cells for 24 h. RNA was extracted using miRNeasy mini kit (Qiagen). Lysis buffer was added to extracted RNA, and then processed on an automated HTG EdgeSeq instrument for probe-capture of validated human miRNAs (2083 miRNA) for 20 h [33]. After probe-capture, the samples were processed for NGS library preparation as previously described [33].…”

“…Lysis buffer was added to extracted RNA, and then processed on an automated HTG EdgeSeq instrument for probe-capture of validated human miRNAs (2083 miRNA) for 20 h [33]. After probe-capture, the samples were processed for NGS library preparation as previously described [33]. miRNA sequencing with Illumina platform was conducted according to HTG instructions [33].…”

Crosstalk between miRNAs and DNA Methylation in Cancer

“…Other biological fluids can be also informative. In urine samples, it is possible to detect miRNAs with diagnostic and prognostic biomarker in esophageal [220], cervical [221], bladder [222][223][224], colorectal [225], and prostate cancers [226].…”