Urine Cytology

Weinstein, Nicole

doi:10.1002/9781119380559.ch39

Cited by 2 publications

(3 citation statements)

References 73 publications

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“…However, inflammation and reactive changes may result in similar cytologic features to neoplastic epithelial cells and it is not always possible to differentiate between them. As a consequence, a definitive diagnosis using urine cytology alone may not be achievable in all cases ( 36 , 37 ). In these instances, the potential of corroborating a diagnosis of UC by detection of the BRAF pathogenic variant as a surrogate marker of malignancy would be a breakthrough.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, there were samples in which molecular testing identified the BRAF pathogenic variant in the urine of cases without cytological evidence of atypia. In many cases, the cytological evaluation of the urinary sediment was inconclusive due to low cellular yield, sample deterioration or atypia relating to inflammation and dysplasia ( 37 ). Inclusion of these samples in the statistical analysis as non-neoplastic likely impacted this finding.…”

Section: Discussionmentioning

confidence: 99%

“…Inclusion of these samples in the statistical analysis as non-neoplastic likely impacted this finding. Samples obtained directly from a catheter have greater success in predicting malignancy ( 37 ). However, urine samples with inflammation, pyuria and/or haematuria could potentially mask evidence of malignancy on cytology.…”

Section: Discussionmentioning

confidence: 99%

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Validation of a Liquid Biopsy Protocol for Canine BRAFV595E Variant Detection in Dog Urine and Its Evaluation as a Diagnostic Test Complementary to Cytology

Gentilini

Palgrave²,

Neta³

et al. 2022

Front. Vet. Sci.

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A significant proportion of canine urothelial carcinomas carry the driver valine to glutamic acid variation (V595E) in BRAF kinase. The detection of V595E may prove suitable to guide molecularly targeted therapies and support non-invasive diagnosis of the urogenital system by means of a liquid biopsy approach using urine. Three cohorts and a control group were included in this multi-step validation study which included setting up a digital PCR assay. This was followed by investigation of preanalytical factors and two alternative PCR techniques on a liquid biopsy protocol. Finally, a blind study using urine as diagnostic sample has been carried out to verify its suitability as diagnostic test to complement cytology. The digital PCR (dPCR) assay proved consistently specific, sensitive, and linear. Using the dPCR assay, the prevalence of V595E in 22 urothelial carcinomas was 90.9%. When compared with histopathology as gold standard in the blind-label cases, the diagnostic accuracy of using the canine BRAF (cBRAF) variation as a surrogate assay against the histologic diagnosis was 85.7% with 92.3% positive predictive value and 80.0% negative predictive value. In all the cases, in which both biopsy tissue and the associated urine were assayed, the findings matched completely. Finally, when combined with urine sediment cytology examination in blind-label cases with clinical suspicion of malignancy, the dPCR assay significantly improved the overall diagnostic accuracy. A liquid biopsy approach on urine using the digital PCR may be a valuable breakthrough in the diagnostic of urothelial carcinomas in dogs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Validation of a Liquid Biopsy Protocol for Canine BRAFV595E Variant Detection in Dog Urine and Its Evaluation as a Diagnostic Test Complementary to Cytology

Gentilini

Palgrave²,

Neta³

et al. 2022

Front. Vet. Sci.

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Relation between Urine Cytological Findings and Renal Function in Patients with Kidney Stones in Taif, Saudi Arabia

Qahtani,

Ismail,

Hagag

et al. 2024

Medicina

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Background and Objectives: Urine serves as a vital diagnostic fluid, and urine cytology analysis plays a crucial role in identifying urinary system illnesses such as bladder cancer and kidney stones. The Paris System for Reporting Urinary Cytology establishes a uniform method for diagnosing urinary tract cancer. This study aimed to provide valuable insights that can inform diagnostic strategies related to kidney stones and ultimately improve patient outcomes via the early detection of the cellular changes associated with kidney stones and their relation to kidney function tests. Materials and Methods: A comparative study was conducted and comprised two groups: group 1, consisting of 50 patients diagnosed with kidney stones, and group 2, comprising 50 patients diagnosed with other kidney diseases. Renal function tests and urinalysis (via the PAP staining of urine cellular deposits to detect nuclear changes) were performed, and the results were analyzed. Results: There was a statistically significant increase in urinary red blood cells, white blood cells, and nuclear reactive atypical changes in urinary sediments of kidney stone patients compared to the patients without stones, while there was a decrease in the estimated glomerular filtration rate (eGFR). eGFR showed a 96.7% specificity in detecting cases with nuclear reactive atypia. Conclusions: eGFR emerges as a reliable diagnostic marker for the comprehensive assessment of kidney stones, particularly when associated with nuclear atypia. The significant correlation between the indicators of chronic kidney disease, such as decreased eGFR, and the presence of kidney stones emphasizes the urgent need for efficient diagnostic practices.

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Urine Cytology

Cited by 2 publications

References 73 publications

Validation of a Liquid Biopsy Protocol for Canine BRAFV595E Variant Detection in Dog Urine and Its Evaluation as a Diagnostic Test Complementary to Cytology

Validation of a Liquid Biopsy Protocol for Canine BRAFV595E Variant Detection in Dog Urine and Its Evaluation as a Diagnostic Test Complementary to Cytology

Relation between Urine Cytological Findings and Renal Function in Patients with Kidney Stones in Taif, Saudi Arabia

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