Urine metabolomics of high-fat diet induced obesity using UHPLC-Q-TOF-MS

Men, Lihui; Pi, Zifeng; Zhou, Yuan; Wei, Mengying; Liu, Yuanyuan; Song, Fengrui; Liu, Zhongying

doi:10.1016/j.jpba.2016.10.012

Cited by 34 publications

(33 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Specifically, we found significant lower concentrations of α-tocopherol, L-phenylalanine and L-tyrosine in omeprazole-treated rat serum. α-tocopherol is a fat-soluble vitamin E with potent antioxidant activity and is involved lipid metabolism 31 , and L-phenylalanine and L-tyrosine are aromatic amino acids and have been shown to decrease in high-fat diet mouse model 32 . In stool, the 20-carbon chain unsaturated fatty acid arachidonic acid and 18-carbon chain saturated fatty acid stearic acid were decreased in omeprazole-treated rat, both fatty acids are involved in lipid metabolism and biosynthesis of unsaturated fatty acid.…”

Section: Discussionmentioning

confidence: 99%

Long-term Proton Pump Inhibitor Administration Caused Physiological and Microbiota Changes in Rats

Yang

Chang

Lin

et al. 2020

Sci Rep

View full text Add to dashboard Cite

proton pump inhibitors (ppis) are used for the long-term treatment of gastroesophageal disorders and the non-prescription medicines for acid reflux. However, there is growing concerns about PPI misuse, overuse and abuse. This study aimed to develop an animal model to examine the effects of long-term use of ppi in vivo. Twenty one Wistar rats were given omeprazole orally or intravenously for 30 days, and caerulein as a positive control. After euthanization, the serum and stool were collected to perform MS-based quantitative analysis of metabolites. We carried out 16S-based profiling of fecal microbiota, assessed the expression of bile acid metabolism regulators and examined the immunopathological characteristics of bile ducts. After long-term PPI exposure, the fecal microbial profile was altered and showed similarity to those observed in high-fat diet studies. The concentrations of several metabolites were also changed in various specimens. Surprisingly, morphological changes were observed in the bile duct, including ductal epithelial proliferation, micropapillary growth of biliary epithelium, focal bile duct stricture formation and bile duct obstruction. these are characteristics of precancerous lesions of bile duct. fXR and RXRα expressions were significantly reduced, which were similar to that observed in cholangiocarcinoma in TCGA and Oncomine databases. We established a novel animal model to examine the effects of long-term use of omeprazole. The gut microbes and metabolic change are consequences of long-term PPI exposure. And the results showed the environment in vivo tends to a high-fat diet. More importantly, we observed biliary epithelial hyperplasia, which is an indicator of a high-fat diet. Proton Pump Inhibitors (PPIs) are one of the most prescribed drugs in many countries. It inhibits the H + /K + ATPase enzyme system in the stomach to suppress acid production, resulting in higher gastric pH. Long-term PPI use is an effective treatment for gastroesophageal disorders and Helicobacter pylori infection, and are taken without prescription to relieve heartburn 1. Despite their increased usage and popularity, over-the-counter (OTC) PPIs such as omeprazole, lansoprazole and pantoprazole are prone to overuse and misuse. There are growing concerns in the medical community about the inappropriate and overuse of PPIs 2-4 .

show abstract

Section: Discussionmentioning

confidence: 99%

Long-term Proton Pump Inhibitor Administration Caused Physiological and Microbiota Changes in Rats

Yang

Chang

Lin

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Nutrition-It is not surprising that the food that someone is eating can have a major impact on a metabolomic profile; in fact, urine metabolites have been shown to correlate with obesity in rats fed a high fat diet [90]. The popular ketogenic diet has also been a recent area of research, with the finding that biochemistry and epigenetic changes have been observed [91], including those that appear to improve with the ketogenic plan.…”

Section: Extrinsic Factorsmentioning

confidence: 99%

Metabolomics in Radiation Biodosimetry: Current Approaches and Advances

et al. 2020

View full text Add to dashboard Cite

Triage and medical intervention strategies for unanticipated exposure during a radiation incident benefit from the early, rapid and accurate assessment of dose level. Radiation exposure results in complex and persistent molecular and cellular responses that ultimately alter the levels of many biological markers, including the metabolomic phenotype. Metabolomics is an emerging field that promises the determination of radiation exposure by the qualitative and quantitative measurements of small molecules in a biological sample. This review highlights the current role of metabolomics in assessing radiation injury, as well as considerations for the diverse range of bioanalytical and sampling technologies that are being used to detect these changes. The authors also address the influence of the physiological status of an individual, the animal models studied, the technology and analysis employed in interrogating response to the radiation insult, and variables that factor into discovery and development of robust biomarker signatures. Furthermore, available databases for these studies have been reviewed, and existing regulatory guidance for metabolomics are discussed, with the ultimate goal of providing both context for this area of radiation research and the consideration of pathways for continued development.

show abstract

“…41 These data suggest that gut microora metabolism in humans is strongly linked to the obesity phenotype. UHPLC-MS and multivariate analyses were utilized to analyze obesity related metabolites in rat urine samples 42 . It successfully identied 20 altered metabolites in urine that correlated with obesity.…”

Section: Metabolomics Application In Obesitymentioning

confidence: 99%