Urine proteomics of primary membranous nephropathy using nanoscale liquid chromatography tandem mass spectrometry analysis

Pang, Liwei; Li, Qianqian; Li, Yan; Liu, Yi; Duan, Nan; Li, Haixia

doi:10.1186/s12014-018-9183-3

Cited by 32 publications

(24 citation statements)

References 40 publications

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“…Twenty-one proteins from the 2D dataset were selected as potential protein candidates for biomarkers of the disease (Table 2). AAT, APOA4 and VIL1 concentrations have previously been shown to increase in blood plasma as CKD progresses [28][29][30][31]. Our data confirm the increased blood serum concentrations of these proteins in patients; whereas only serum AAT values correlated with elevated levels of creatinine and cytokines.…”

Section: Discussionsupporting

confidence: 85%

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Proteomic Analysis of Human Serum from Patients with Chronic Kidney Disease

et al. 2020

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Chronic kidney disease (CKD) is an important public health problem in the world. The aim of our research was to identify novel potential serum biomarkers of renal injury. ELISA assay showed that cytokines and chemokines IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, Eotaxin, FGFb, G-CSF, GM-CSF, IP-10, MCP-1, MIP-1α, MIP-1β, PDGF-1bb, RANTES, TNF-α and VEGF were significantly higher (R > 0.6, p value < 0.05) in the serum of patients with CKD compared to healthy subjects, and they were positively correlated with well-established markers (urea and creatinine). The multiple reaction monitoring (MRM) quantification method revealed that levels of HSP90B2, AAT, IGSF22, CUL5, PKCE, APOA4, APOE, APOA1, CCDC171, CCDC43, VIL1, Antigen KI-67, NKRF, APPBP2, CAPRI and most complement system proteins were increased in serum of CKD patients compared to the healthy group. Among complement system proteins, the C8G subunit was significantly decreased three-fold in patients with CKD. However, only AAT and HSP90B2 were positively correlated with well-established markers and, therefore, could be proposed as potential biomarkers for CKD.

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Section: Discussionsupporting

confidence: 85%

“…Exogenous AAT inhibited apoptosis and inflammation in renal reperfusion [28] and reduced the urine kidney injury molecule-1 (KIM-1) concentration [30]. Several studies have demonstrated increased blood plasma AAT concentrations in hypoxia [31] and acute myocardial infarction [48,49].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Human Serum from Patients with Chronic Kidney Disease

et al. 2020

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“…With class‐specific inhibitors, serine protease activity accounted for more than 80% of the total protease activity, with the remainder being aspartate, cysteine, and metalloproteases. Using a proteomic approach, serine proteases seen in nephrotic syndrome were typically those that circulate in soluble form in the plasma and belong to the coagulation and complement cascade . Therefore, proteasuria in nephrotic syndrome reflects the translocation and excretion of active proteases from the plasma compartment into the urine.…”

Section: Proteasuria In Patients With Nephrotic Syndromementioning

confidence: 99%

Proteasuria—The impact of active urinary proteases on sodium retention in nephrotic syndrome

Artunc¹,

Wörn²,

Schork³

et al. 2019

Acta Physiologica

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Sodium retention and extracellular volume expansion are typical features of patients with nephrotic syndrome. In recent years, from in vitro data, endoluminal activation of the epithelial sodium channel (ENaC) by aberrantly filtered serine proteases has been proposed as an underlying mechanism. Recently, this concept was supported in vivo in nephrotic mice that were protected from proteolytic ENaC activation and sodium retention by the use of aprotinin for the pharmacological inhibition of urinary serine protease activity. These and other findings from studies in both rodents and humans highlight the impact of active proteases in the urine, or proteasuria, on ENaC-mediated sodium retention and edema formation in nephrotic syndrome. Targeting proteasuria could become a therapeutic approach to treat patients with nephrotic syndrome. However, pathophysiologically relevant proteases remain to be identified. In this review, we introduce the concept of proteasuria to explain tubular sodium avidity and conclude that proteasuria can be considered as a key mechanism of sodium retention in patients with nephrotic syndrome. K E Y W O R D Saprotinin, ENaC, nephrotic syndrome, proteasuria, proteinuria, proteolysis, serine protease

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“…Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a noninvasive detection method used in urine proteomics that can provide qualitative and quantitative assessments of the proteome and offer clues for biological processes in systems. LC-MS/MS has been applied in the proteomic analysis of various kidney diseases, such as membranous nephropathy [8], acute kidney injury [9], lupus nephritis [10] and IgA nephropathy (IgAN) [11]. Data-independent acquisition (DIA) by LC-MS/MS, which enables comprehensive mapping of the urinary proteome, has sufficient throughput to perform biomarker discovery studies, as all identified proteins can be precisely quantified in hundreds of samples [12].…”

Section: Introductionmentioning

confidence: 99%

Urinary proteomics of Henoch-Schönlein purpura nephritis in children using liquid chromatography-tandem mass spectrometry

Fang

Ming

et al. 2020

Clin Proteom

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Background: Henoch-Schönlein purpura nephritis (HSPN) is the principal cause of morbidity and mortality in children with Henoch-Schönlein purpura (HSP). However, the criteria for risk assessment currently used is not satisfactory. The urine proteome may provide important clues to indicate the development of HSPN. Methods: Here, we detected and compared the urine proteome of patients with HSPN and healthy controls by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the data-independent acquisition (DIA) mode. The differentially expressed proteins were analysed by gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. For validation, enzyme-linked immunosorbent assay (ELISA) was used to analyse the selected proteins. Results: A total of 125 proteins (29 upregulated and 96 downregulated) were found to be differentially expressed in children with HSPN compared with the controls. Forty-one proteins were predicted to have direct interactions. The enriched pathways mainly included focal adhesion, cell adhesion molecules, the PI3K-Akt signalling pathway, ECMreceptor interactions and so on. Cell adhesion related to the pathogenesis of HSPN was the main biological process identified in this study. The decrease in two proteins (integrin beta-1 and tenascin) was validated by ELISA. Conclusions: Our study provides new insights into the assessment of HSPN progression in children, as well as new potential biomarkers. The data confirm the value of the urinary proteome in capturing the emergence of HSPN.

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Urine proteomics of primary membranous nephropathy using nanoscale liquid chromatography tandem mass spectrometry analysis

Cited by 32 publications

References 40 publications

Proteomic Analysis of Human Serum from Patients with Chronic Kidney Disease

Proteomic Analysis of Human Serum from Patients with Chronic Kidney Disease

Proteasuria—The impact of active urinary proteases on sodium retention in nephrotic syndrome

Urinary proteomics of Henoch-Schönlein purpura nephritis in children using liquid chromatography-tandem mass spectrometry

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