Urine real-time polymerase chain reaction detection for children virus pneumonia with acute human cytomegalovirus infection

Abstract

BackgroundHuman cytomegalovirus (HCMV) is an important pathogen of viral pneumonia in children. The diagnosis of acute HCMV infection is complicated and difficult.MethodsClinical and laboratory data of 6063 hospitalized children with respiratory infection and 509 with respiratory virus infection alone were retrospectively analyzed. Urine and respiratory specimens of 186 hospitalized children with pneumonia were also prospectively collected. Real-time polymerase chain reaction (PCR) and a chemiluminescent assay were used to detect HCMV DNA copy number, the pp65 gene, and HCMV IgM.ResultsThe patients with respiratory virus infection alone and those with pulmonary HCMV infection (n = 422) were mostly children aged <6 months old (82.91%, 422/509). The accuracy of urine HCMV DNA (82.32%) was higher than that of HCMV IgM (67.78%), indicating that PCR of urine samples is suitable for determining pediatric acute pulmonary HCMV infection. There was no significant difference in detecting HCMV DNA or the pp65 gene between urinary and respiratory specimens (P > 0.05) in 186 pediatric pneumonia cases. The accuracy of the pp65 gene measured in urine for determining acute pulmonary HCMV infection was the highest (93.01%).ConclusionsOur study shows a novel method for investigating acute pulmonary HCMV infection in children by using real-time PCR and non-invasive samples. This study also highlights the superiority and potential use of the pp65 gene as an important target for the diagnosis of acute pulmonary HCMV infection.

Epidemiological characteristics of human cytomegalovirus infection and glycoprotein H genotype in Chinese children

“…A previous study suggested that children aged <6 months are the major population among HCMVinfected children with pneumonia. 17 During our two-year prospective study, the epidemiological data showed that children aged under one year were the most susceptible population with a peak incidence among the children in the age range of three months to one year. Among all HCMVpositive children, the proportion of the children aged <6 months was 68.7%, whereas that of those aged above 6 months was 31.3%.…”

Human cytomegalovirus prevalence and distribution of glycoprotein B, O genotypes among hospitalized children with respiratory infections in West China, 2009–2014

“…Previous studies have been conducted on the prevalence of HCMV in paediatric population in China and other parts of the world . The results were inconsistent, due to different inclusion criteria, test methods and specimens resources.…”

Transalkylation of Higher Trifluoromethylated Fullerenes with C70: A Pathway to New Addition Patterns of C70(CF3)8

“…For example, viral cultures waste time and energy 11, and nucleic acid testing requires special equipment and high costs 12, 13. Furthermore, antibody testing is unsatisfactory because the CMV antibody has a short lifetime and interferences in the tests 14, 15. The CMV pp65 antigen emerges during the early stages of a CMV infection and is widely distributed in human vascular endothelial cells, peripheral blood leukocytes and human body fluids[16].…”