Urine Single-Cell RNA Sequencing in Focal Segmental Glomerulosclerosis Reveals Inflammatory Signatures

Abstract

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Urinary single-cell sequencing captures intrarenal injury and repair processes in human acute kidney injury

“…Next, we wanted to know whether any of these TEC phenotypes are specific for the damage introduced by AKI: We compared our data with recently published urinary scRNAseq data of chronic glomerular diseases DN (18) and FSGS (19) (Suppl. Fig.…”

“…Together with data from DN (18) and FSGS (19) patients, urinary scRNAseq has now been tested in three credible cohorts across different diseases. These studies revealed a high variability of the viable cell count across samples.…”

“…The urinary cellular signal – independent from disease type or severity – is dominated by leukocytes and urogenital epithelia. (18, 19). Therefore, most urinary cellular studies, single-cell (48) or otherwise (14, 16, 49), have focused on analyses of urinary immune cells.…”

“…scRNAseq data of 3 human bladder samples (39) (GSE129845) was downloaded from the National Center for the Biotechnology Information Database (NCBI) / Gene expression omnibus (GEO) repository. Urine scRNAseq data including 16 samples from 5 patients with diabetic nephropathy (18) (GSE157640) and 23 samples from 12 patients focal segmental glomerulosclerosis (FSGS) (19) (GSE176465) were also downloaded from the NCBI/GEO repository. Data integration/analysis/dimensional reduction and clustering of these datasets was done exactly as with in urine AKI samples.…”

Urine Single-Cell RNA Sequencing in Focal Segmental Glomerulosclerosis—Hope for the Future

“…6 scRNA-seq has also been used to characterize the urinary sediment of patients hospitalized with COVID-19 7 and to define the cellular profile of healthy urine. 8 In this issue of KI Reports, Latt et al 9 used a pilot approach to test the utility scRNA-seq in characterizing the urinary transcriptomic profile of 12 subjects with FSGS. The findings reveal the presence of immune cells, podocytes, myofibroblasts, and tubular cells with distinct expression profiles.…”