Urogenital Myiasis Caused by Megaselia scalaris (Diptera: Phoridae): A Case Report

Singh, TN; Rana, Dimple

doi:10.1093/jmedent/26.3.228

Cited by 49 publications

(25 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Identification of the species of maggots prior to treatment is important since not all types of myiasis are benign (Singh and Rana, 1989). The present work focused on an ordinary microscopy for identification of the larvae.…”

Section: Discussionmentioning

confidence: 99%

Human Urogenital Myiasis Caused by Psychoda Species Larvae: Report of Five Cases and Morphological Studies

Yones¹

2014

J. adv. parasitol.

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Human Urogenital Myiasis Caused by Psychoda Species Larvae: Report of Five Cases and Morphological Studies

Yones¹

2014

J. adv. parasitol.

View full text Add to dashboard Cite

“…In addition to causing myiasis in humans (Trape et al 1982, Singh et al 1988, Singh & Rana 1989, it has also been reported as a forensically-important fly (e.g. Barnes 1990, Disney 1994.…”

mentioning

confidence: 99%

Surface ultrastructure of third-instar Megaselia scalaris (Diptera: Phoridae)

Sukontason

Lertthamnongtham

et al. 2002

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

We describe some ultrastructure of the third-instar Megaselia scalaris (Diptera: Phoridae) using scanning electron microscopy, with the cephalic segment, anterior spiracle and posterior spiracle being emphasized. This study provides the taxonomic information of this larval species, which may be useful to differentiate from other closelyrelated species.Key words: Megaselia scalaris -ultrastructure -third-instar -scanning electron microscopy -forensic entomology Megaselia scalaris (Loew), humpbacked fly, is an insect of medical importance worldwide. In addition to causing myiasis in humans (Trape et al. 1982, Singh et al. 1988, Singh & Rana 1989, it has also been reported as a forensically-important fly (e.g. Barnes 1990, Disney 1994. Larvae of this species have more recently been reported from both exposed human corpses and within a tightly sealed corpse (Greenberg & Wells 1998). Thus, the identification of this fly larva to species is mandatory to improve the accuracy of a forensic investigation, if this larva is present in a corpse and could be used as entomological evidence.The immature and adult stages of M. scalaris have been previously described based on light microscopy (Zumpt 1965, Tumrasvin et al. 1977, Kaneko et al. 1978, Liu & Greenberg 1989, and scanning electron microscopy (SEM) has been used to describe the egg (Greenberg & Wells 1998). We report, herein, some details of the surface ultrastructure of the third-instar M. scalaris with the aid of SEM. Particular attention was given to the morphology of the cephalic segment, anterior and posterior spiracles, in order to point out some taxonomic significance from the other Megaselia species.The third-instar M. scalaris, which was obtained from wild-caught females that came to lay eggs in a tray baited with fresh pork liver, was set up in the Department of Parasitology, Faculty of Medicine, Chiang Mai University, Thailand. The eggs were identified as M. scalaris according to the morphological features demonstrated by Greenberg and Wells (1998 from the egg, it was reared with fresh pork liver until reaching the third-instar. Some larvae were collected from the colony and washed several times with normal saline solution. They were killed by placing in hot water (≈70°C) for a few min and then fixed in a fixative agent consisting of 2.5% glutaraldehyde at 4°C for 24 h. The fixed larvae were then subjected to postfixation in 1% osmium tetroxide and dehydration in a graded alcohol series that was followed by treatment in acetone and critical-point drying. They were then mounted on stubs, sputter-coated with gold, and viewed with a JEOL-JSM840A scanning electron microscope. Photomicrographs were made using Kodak® Verichrome Panchromatic film VP 200.The third-instar M. scalaris is creamy white and ≈4 mm in length.

show abstract

“…Several workers reported cases of intestinal myiasis due to M. scalaris [7][8][9]. Hira et al [10] described in 2004 a case of nosocomial myiasis in Kuwait caused by M. scalaris.…”

Section: Discussionmentioning

confidence: 99%

“…Hira et al [10] described in 2004 a case of nosocomial myiasis in Kuwait caused by M. scalaris. The first report of human urogenital myiasis due to a species of Megaselia was represented in 1978 by Disney and Kurahashi [11], and then more cases were reported [12][13][14]. Other workers studied invasion of ticks [15], bananas [16], and snacks [17] by the larvae of M. scalaris.…”

Section: Discussionmentioning

confidence: 99%

A Laboratory-Based Study for First Documented Case of Urinary Myiasis Caused by Larvae of Megaselia scalaris (Diptera: Phoridae) in Saudi Arabia

Wakid

2008

Korean J Parasitol

View full text Add to dashboard Cite

: Megaselia scalaris (Diptera: Phoridae) is one of the medically important insects. Maggots from a urine sample of a 5-year-old Saudi girl were examined microscopically for identification. These maggots were cultured to become adult flies. Larvae and adults were identified using standard keys. Protozoan flagellates were obtained from the gut of the larvae. This is the first report of M. scalaris as a causative agent of urinary human myiasis in Saudi Arabia.

show abstract

Urogenital Myiasis Caused by Megaselia scalaris (Diptera: Phoridae): A Case Report

Cited by 49 publications

References 0 publications

Human Urogenital Myiasis Caused by Psychoda Species Larvae: Report of Five Cases and Morphological Studies

Human Urogenital Myiasis Caused by Psychoda Species Larvae: Report of Five Cases and Morphological Studies

Surface ultrastructure of third-instar Megaselia scalaris (Diptera: Phoridae)

A Laboratory-Based Study for First Documented Case of Urinary Myiasis Caused by Larvae of Megaselia scalaris (Diptera: Phoridae) in Saudi Arabia

Contact Info

Product

Resources

About