Uropod elongation is a common final step in leukocyte extravasation through inflamed vessels

Hyun, Young-Min; Sumagin, Ronen; Sarangi, Pranita P.; Lomakina, Elena B.; Overstreet, Michael G.; Baker, Christina M.; Fowell, Deborah J.; Waugh, Richard E.; Sarelius, Ingrid H.; Kim, Min‐Soo

doi:10.1084/jem.20111426

Cited by 117 publications

(118 citation statements)

References 69 publications

Supporting

Mentioning

113

Contrasting

Order By: Relevance

“…In contrast, LFA-1-deficient leukocytes did not elongate and detached quickly (58). These data suggest that once extravasated, LFA-1/ICAM-1 maintain binding of the uropod to the abluminal surface of endothelium while VLA-3 mediates movement of the front of the cell and thus facilitates leukocyte emigration through the basement membrane (58). Outside-in signaling in leukocyte recruitment.…”

Section: Leukocyte Recruitmentmentioning

confidence: 99%

“…Following subendothelial crawling, leukocytes maintain connection to the endothelial abluminal surface, leading to uropod elongation and a significantly delayed detachment (up to .20 min) of neutrophils, monocytes, and lymphocytes (58). Leukocytes in mice deficient in VLA-3 were found to have impaired detachment, as they failed to elongate by active movement of the leading edge of the cell (58). In contrast, LFA-1-deficient leukocytes did not elongate and detached quickly (58).…”

Section: Leukocyte Recruitmentmentioning

confidence: 99%

“…Recent studies have revealed that extravasated neutrophils crawl in the space between the endothelial abluminal surface and pericytes in a Mac-1-and LFA-1-dependent manner (57). Following subendothelial crawling, leukocytes maintain connection to the endothelial abluminal surface, leading to uropod elongation and a significantly delayed detachment (up to .20 min) of neutrophils, monocytes, and lymphocytes (58). Leukocytes in mice deficient in VLA-3 were found to have impaired detachment, as they failed to elongate by active movement of the leading edge of the cell (58).…”

Section: Leukocyte Recruitmentmentioning

confidence: 99%

“…Leukocytes in mice deficient in VLA-3 were found to have impaired detachment, as they failed to elongate by active movement of the leading edge of the cell (58). In contrast, LFA-1-deficient leukocytes did not elongate and detached quickly (58). These data suggest that once extravasated, LFA-1/ICAM-1 maintain binding of the uropod to the abluminal surface of endothelium while VLA-3 mediates movement of the front of the cell and thus facilitates leukocyte emigration through the basement membrane (58).…”

Section: Leukocyte Recruitmentmentioning

confidence: 99%

See 3 more Smart Citations

Integrin Regulation during Leukocyte Recruitment

Herter

Zarbock

2013

The Journal of Immunology

171

138

View full text Add to dashboard Cite

Integrins are recognized as vital players in leukocyte recruitment. Integrin malfunction causes severe disease patterns characterized by the inability to fight pathogens. Although inflammatory reactions are beneficial and necessary for host defense, these reactions have to be controlled to prevent tissue destruction and harmful sequelae. In this review, we discuss the different signaling pathways leading to the change of integrin adhesiveness in neutrophils, monocytes, and lymphocytes. We thereby focus on the importance of integrin activation for the different steps of the leukocyte recruitment cascade, including rolling, adhesion, postadhesion strengthening, intravascular crawling, and transmigration, as each step necessitates the proper functioning of a distinct set of integrin molecules that has to be activated specifically. Additionally, we discuss endogenous mechanisms that balance and counteract integrin activation and limit leukocyte recruitment at the site of inflammation. Further insight into these complex mechanisms may provide new approaches for developing new anti-inflammatory therapies.

show abstract

Section: Leukocyte Recruitmentmentioning

confidence: 99%

Section: Leukocyte Recruitmentmentioning

confidence: 99%

Section: Leukocyte Recruitmentmentioning

confidence: 99%

Section: Leukocyte Recruitmentmentioning

confidence: 99%

See 2 more Smart Citations

Integrin Regulation during Leukocyte Recruitment

Herter

Zarbock

2013

The Journal of Immunology

171

138

View full text Add to dashboard Cite

show abstract

“…The decrease in WBCs in blood in the EZZ and Esomeprazole-treated ulcerated rats could possibly be due to migration of some of these cells from blood (leucocyte extravasation) into areas around the damaged mucosal tissue as part of innate immune response in the body's defense mechanism (Hyun et al, 2012). The decrease in RBC and HB in blood could possibly be the result of blood lost from ulcers developed in the stomach (Seibert, 2012).…”

Section: Discussionmentioning

confidence: 99%

Gastroprotective Effect and Safety Assessment of <i>Zanthoxylum Zanthoxyloides</i> (Lam) Waterm Root Bark Extract

Günther¹

2012

American Journal of Pharmacology and Toxicology

View full text Add to dashboard Cite

Problem statement: Gastrointestinal ulcers account significantly for morbidity and mortality in Ghana. The study therefore investigated the gastroprotective effect of an ethanolic root bark extract of Zanthoxylum zanthxyloides and its safety for us. Approach: Gastric ulceration was induced in Sprague-Dawley rats with Indomethacin (25 mg kg Index and Curative Ratio were determined. A histological study of the gastric mucosa was also performed. The extract's effect on an isolated guinea-pig ileum preparation was investigated to elucidate its possible mechanism of action. Safety assessments involving, organ weight to body weight ratio determination, hematological analysis and liver function tests were performed. Results: The extract significantly decreased (p≤0.001) the number of ulcers per stomach and the Ulcerative Index (similar to Esomeprazole) and significantly increased (p≤0.001) the Curative Ratio in a dose-dependent manner. It completely corrected the architectural distortions caused by gastric ulceration and inhibited significantly (p≤0.001) the contractile responses of the isolated guinea-pig ileum to Acetylcholine, Nicotine and Histamine in a manner comparable to reference antagonists. The extract had no significant effect (p>0.05) on organ weight to body ratio and hematological profile. Plasma levels of Alanine transaminase and Alkaline phosphatase decreased significantly (p≤0.001) in extract and Esomeprazole-treated ulcerated rats. Levels of Gamma-glutamyl transferase, Total bilirubin (direct and indirect) however increased significantly (p≤0.01-0.001). Conclusion/Recommendations: Per the findings, the ethanolic root bark extract of Zanthoxylum zanthoxyloides has gastroprotective effect in Sprague-Dawley rats working possibly via antimuscarinic or antihistaminic mechanism. It however has a potential of causing cholestasis hence liver function should be monitored.

show abstract