Urothelial umbrella cells of human ureter are heterogeneous with respect to their uroplakin composition: different degrees of urothelial maturity in ureter and bladder?

Riedel, Ina; Liang, Feng; Deng, Fang−Ming; Tu, Liyu; Kreibich, Gert; Wu, Xue-Ru; Sun, Tung‐Tien; Hergt, Michaela; Moll, Roland

doi:10.1016/j.ejcb.2004.12.011

Cited by 42 publications

(31 citation statements)

References 41 publications

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“…This area (apical side of the superficial cells) is characterized by rigid membranous plaques which are essential to maintain impermeability and integrity of the urothelium 53 ; L1CAM might contribute to the maintenance of these plaques; however, this hypothesis is poorly supported by our data. Apart from the MET pathway, L1CAM has shown to govern an epithelial-to-mesenchymal transition (EMT) during both embryogenesis and carcinogenesis.…”

Section: Discussioncontrasting

confidence: 58%

L1CAM in the Early Enteric and Urogenital System

Pechriggl

Concin

Blumer

et al. 2016

J Histochem Cytochem.

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Article IntroductionIn 1984, Rathjen and Schachner identified a new cell adhesion molecule (L1 cell adhesion molecule [L1CAM]) in neurons that turned out to be a key player in the neuronal development of mice where it governs neuronal outgrowth, migration, and axonal guidance. 1 L1CAM is a transmembrane protein belonging to the immunoglobulin superfamily, interacting with several ligands such as integrins, neuropilins, contactin 1 and 2, CD24, as well as with its shed form. 2Apart from its established function, studies in rodents and cell lines showed that L1CAM has an essential role in the formation and maintenance of epithelia, which is achieved via two contrary modes of action: On the one hand, L1CAM is important for both the establishment of cell-cell complexes and the SummaryL1 cell adhesion molecule (L1CAM) is a transmembrane molecule belonging to the L1 protein family. It has shown to be a key player in axonal guidance in the course of neuronal development. Furthermore, L1CAM is also crucial for the establishment of the enteric and urogenital organs and is aberrantly expressed in cancer originating in these organs. Carcinogenesis and embryogenesis follow a lot of similar molecular pathways, but unfortunately, comprehensive data on L1CAM expression and localization in human developing organs are lacking so far. In the present study we, therefore, examined the spatiotemporal distribution of L1CAM in the early human fetal period (weeks 8-12 of gestation) by means of immunohistochemistry and in situ hybridization (ISH). In the epithelia of the gastrointestinal organs, L1CAM localization cannot be observed in the examined stages most likely due to their advanced polarization and differentiation. Despite these results, our ISH data indicate weak L1CAM expression, but only in few epithelial cells. The genital tracts, however, are distinctly L1CAM positive throughout the entire fetal period. We, therefore, conclude that in embryogenesis L1CAM is crucial for further differentiation of epithelia. (J Histochem Cytochem 65:21-32, 2017)

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Section: Discussioncontrasting

confidence: 58%

L1CAM in the Early Enteric and Urogenital System

Pechriggl

Concin

Blumer

et al. 2016

J Histochem Cytochem.

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“…21 Second, in vitro studies have shown that urothelium from the two sites differs in uroplakin content, keratin expression pattern, growth potential, and propensity to keratinize. 22 Extracellular matrix-associated proteins with counter-adhesive properties respond differently in ureteric and bladder urothelial cells. 23 Mono-and dinucleotide microsatellite instability, a feature of tumors with deficient mismatch repair, is more common in upper than lower urinary tract cancers, 24,25 and these tumors have more extensive methylation than bladder cancers.…”

Section: Discussionmentioning

confidence: 99%

Randomized Phase III Study Comparing Paclitaxel/Cisplatin/ Gemcitabine and Gemcitabine/Cisplatin in Patients With Locally Advanced or Metastatic Urothelial Cancer Without Prior Systemic Therapy: EORTC Intergroup Study 30987

Bellmunt

Maase²,

Mead³

et al. 2012

JCO

422

239

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PurposeThe combination of gemcitabine plus cisplatin (GC) is a standard regimen in patients with locally advanced or metastatic urothelial cancer. A phase I/II study suggested that a three-drug regimen that included paclitaxel had greater antitumor activity and might improve survival.Patients and MethodsWe conducted a randomized phase III study to compare paclitaxel/cisplatin/gemcitabine (PCG) with GC in patients with locally advanced or metastatic urothelial carcinoma. Primary outcome was overall survival (OS). Secondary outcomes were progression-free survival (PFS), overall response rate, and toxicity.ResultsFrom 2001 to 2004, 626 patients were randomly assigned; 312 patients were assigned to PCG, and 314 patients were assigned to GC. After a median follow-up of 4.6 years, the median OS was 15.8 months on PCG versus 12.7 months on GC (hazard ratio [HR], 0.85; P = .075). OS in the subgroup of all eligible patients was significantly longer on PCG (3.2 months; HR, 0.82; P = .03), as was the case in patients with bladder primary tumors. PFS was not significantly longer on PCG (HR, 0.87; P = .11). Overall response rate was 55.5% on PCG and 43.6% on GC (P = .0031). Both treatments were well tolerated, with more thrombocytopenia and bleeding on GC than PCG (11.4% v 6.8%, respectively; P = .05) and more febrile neutropenia on PCG than GC (13.2% v 4.3%, respectively; P < .001).ConclusionThe addition of paclitaxel to GC provides a higher response rate and a 3.1-month survival benefit that did not reach statistical significance. Novel approaches will be required to obtain major improvements in survival of incurable urothelial cancer.

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“…The specific secondary antibodies and protocols used for immunolocalization by light and electron microscopy of cryostat-sectioned material and by confocal laser-scanning immunofluorescence microscopy were as described (e.g., Langbein et al 2002Langbein et al , 2003Straub et al 2003; for conventional electron microscopy, see Schmelz et al 1994;Langbein et al 2002). For light-microscopic immunolocalization of antigens in formaldehyde-fixed and paraffin-embedded material, the "antigen retrieval" technique by microwave treatment was used essentially as described (e.g., Shi et al 2000;Peitsch et al 2005;Riedel et al 2005).…”

Section: Antibodies and Immunolocalizationmentioning

confidence: 99%

The complexus adhaerens of mammalian lymphatic endothelia revisited: a junction even more complex than hitherto thought

et al. 2005

Self Cite

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The significance of a special kind of VE-cadherin-based, desmoplakin- and plakoglobin-containing adhering junction, originally identified in certain endothelial cells of the mammalian lymphatic system (notably the retothelial cells of the lymph node sinus and a subtype of lining endothelial cells of peripheral lymphatic vessels), has been widely confirmed and its importance in the formation of blood and lymph vessels has been demonstrated in vivo and in vitro. We have recently extended the molecular and structural characterization of the complexus adhaerens and can now report that it represents a rare and special combination of components known from three other major types of cell junction. It comprises zonula adhaerens proteins (VE-cadherin, alpha- and beta-catenin, protein p120(ctn), and afadin), desmosomal plaque components (desmoplakin and plakoglobin), and tight-junction proteins (claudin-5 and ZO-1) and forms junctions that vary markedly in size and shape. The special character and the possible biological roles of the complexus adhaerens and its unique ensemble of molecules in angiogenesis, immunology, and oncology are discussed. The surprising finding of claudin-5 and protein ZO-1 in substructures of retothelial cell-cell bridges, i.e. structures that do not separate different tissues or cell layer compartments, suggests that such tight-junction molecules are involved in functions other than the "fence" and "barrier" roles of zonulae occludentes.

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Urothelial umbrella cells of human ureter are heterogeneous with respect to their uroplakin composition: different degrees of urothelial maturity in ureter and bladder?

Cited by 42 publications

References 41 publications

L1CAM in the Early Enteric and Urogenital System

L1CAM in the Early Enteric and Urogenital System

Randomized Phase III Study Comparing Paclitaxel/Cisplatin/ Gemcitabine and Gemcitabine/Cisplatin in Patients With Locally Advanced or Metastatic Urothelial Cancer Without Prior Systemic Therapy: EORTC Intergroup Study 30987

The complexus adhaerens of mammalian lymphatic endothelia revisited: a junction even more complex than hitherto thought

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