Ursolic acid, a potential PPARγ agonist, suppresses ovalbumin-induced airway inflammation and Penh by down-regulating IL-5, IL-13, and IL-17 in a mouse model of allergic asthma

Kim, Seung-Hyung; Hong, Jung-Hee; Lee, Young‐Cheol

doi:10.1016/j.ejphar.2012.11.033

Cited by 94 publications

(55 citation statements)

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“…Quercetin and rutin also found in extract at higher concentration, that are known to exhibit potent antiasthmatic effect via inhibition of the recruitment of eosinophills and neutrophils into the lung, the production of histamine, phospholipase A2 and eosinophil peroxidase (Chan Hun et al, 2007). In addition, the plant M. serratulum reported to possess the bioactive compounds: Triterpenoid, ursolic acid and iridoid glycoside myxopyroside they have several pharmacological activities including antimicrobial, antiasthmatic, antiallergic and anti-inflammatory, cardiovascular and anti-cancer effect (Wozniak et al, 2015;Kim et al, 2013). The potency of several iridoid derivatives on smooth muscle were reported (Chung et al, 1980;Breschi et al, 1992;Rojas et al, 2000) which were in support of our result.…”

Section: Concentration Of Extractsupporting

confidence: 81%

Bronchodilatory effect of <i>Myxopyrum serratulum</i> in animal model

Maruthamuthu

Kandasamy

2017

Bangladesh J Pharmacol

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Research ArticleBronchodilatory effect of Bronchodilatory effect of Bronchodilatory effect of Myxopy-MyxopyMyxopyrum serratulum rum serratulum rum serratulum in animal model in animal model in animal model BJP IntroductionBronchial asthma is a chronic inflammatory disease of the airway. Infiltration of various inflammatory cells such as eosinophil, macrophages and lymphocytes into the airway causes asthma and bronchitis (Patel et al., 2013).Bronchodilator drugs works by rapid reversal of the airway obstruction in asthmatics by directly acting on airway smooth muscle (Church and Hiroi, 1987). Drugs derived from natural sources are generally considered no adverse effects when compared to synthetic drugs, therefore, it might serve as better alternative for many chronic diseases such as cancer, infections and endemic diseases like asthma, bronchitis and many others (WHO, 2000). Plant extract and secondary metabolites derived from plant can directly influence the production and activation of inflammatory mediators, seconddary messengers and the expression of transcription factors (Calixto et al., 2004).Myxopyrum serratulum belongs to large shrub mainly found in Kerala at a altitude of about 600-900 m. It is commonly known as chaturamulla and traditionally, dried and powdered leaves of the plant was mixed with ghee as a remedy for asthma, cough and nerves complaints apart from which they were also used for the treatment of fever, headache and ear diseases (Wealth of India, 2011).According to earlier reports, the plant possesses antiinflammatory, antiarthritic ), antioxidant (Sheelarani et al., 2013), antipyretic (Vanughese et al., 2015, wound healing (Gopalakrishnan and Rajameena, 2013) and antimicrobial (Gopalakrishnan et al., 2012) properties.The phytochemical studies on M. serratulum revealed AbstractThe plant Myxopyrum serratulum is traditionally claimed to relieve asthma and cough. The present study was undertaken to evaluate the bronchodilatory effect of the methanolic extract of M. serratulum on histamine-induced bronchospasm by in vivo and the inhibitory effect of the extract on histaminecontracted tracheal chain and ileum by in vitro guinea pig model. Additionally, the relaxant effect of four cumulative concentrations of the extract (0.25, 0.5, 0.7 and 1.0 g%) was assessed using precontracted tracheal chain under different conditions. The extract (400 mg/kg) prolonged the preconvulsive time to 102.3 ± 3.8 sec when compared to saline and standard chlorpheniramine maleate as 121.3 ± 4.5 sec (p<0.05). The extract also possessed significant inhibitory effect on histamine-contracted guinea pig ileum and tracheal chain and also exhibited significant relaxation effect on precontracted tracheal chain of guinea pig models contracted by 60 mM KCl (p<0.001) and 10 µM methacholine (p<0.001) when compared with standard theophylline. Article Info

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Section: Concentration Of Extractsupporting

confidence: 81%

Bronchodilatory effect of <i>Myxopyrum serratulum</i> in animal model

Maruthamuthu

Kandasamy

2017

Bangladesh J Pharmacol

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“…32 Penh was determined based on the airway response to methacholine inhalation. Examination of the airway responses to methacholine before and after AgNP inhalation and OVA challenge demonstrated that OVA sensitization alone induced an insignificant alteration in Penh (relative to the responses in the control mice) and significantly increased OVA-specific IgE production in the allergic mice compared to the levels observed before AgNP exposure and OVA challenge.…”

Section: Discussionmentioning

confidence: 99%

Allergenicity and toxicology of inhaled silver nanoparticles in allergen-provocation mice models

Chuang¹,

Hsiao²,

Wu³

et al. 2013

IJN

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Silver nanoparticles (AgNP) have been associated with the exacerbation of airway hyperresponsiveness. However, the allergenicity and toxicology of AgNP in healthy and allergic individuals are unclear. We investigated the pathophysiological responses to AgNP inhalation in a murine model of asthma. Continuous and stable levels of 33 nm AgNP were maintained at 3.3 mg/m 3 during the experimental period. AgNP exposure concomitant with ovalbumin challenge increased the enhanced pause (Penh) in the control and allergic groups. AgNP evoked neutrophil, lymphocyte and eosinophil infiltration into the airways and elevated the levels of allergic markers (immunoglobulin E [IgE] and leukotriene E 4 [LTE 4 ]), the type 2 T helper (Th2) cytokine interleukin-13 (IL-13), and oxidative stress (8-hydroxy-2′-deoxyguanosine [8-OHdG]) in healthy and allergic mice. Bronchocentric interstitial inflammation was observed after AgNP inhalation. After inhalation, the AgNP accumulated predominantly in the lungs, and trivial amounts of AgNP were excreted in the urine and feces. Furthermore, the AgNP induced inflammatory responses in the peritoneum. The inhalation of AgNP may present safety concerns in healthy and susceptible individuals.

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“…UA is pentacyclic triterpenoid that is ubiquitously expressed in most plant species [8] . UA has been shown to exert anti-cancer [9] , anti-oxidative [10] , and anti-inflammatory [11] effects, in addition to conferring benefits in cognitive function [12] , asthma [13] , and colon cancer [14,15] . Of note, Pathak et al reported that UA inhibited activation of the STAT3 pathway, leading to the suppression of proliferation in human multiple myeloma cells [16] .…”

Section: Introductionmentioning

confidence: 99%

Ursolic acid ameliorates autoimmune arthritis via suppression of Th17 and B cell differentiation

Baek

Lee

et al. 2014

Acta Pharmacol Sin

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Aim: Ursolic acid (UA) is a pentacyclic triterpenoid found in most plant species, which has been shown anti-inflammatory and antioxidative activities. In this study, we examined the effects of UA on collagen-induced arthritis (CIA) in mice, and to identify the mechanisms underlying the effects. Methods: CIA was induced in mice. Two weeks later, the mice were treated with UA (150 mg/kg, ip, 3 times per week) for 4 weeks. The expression of cytokines and oxidative stress markers in joint tissues was measured with immunohistochemistry. The numbers of CD4 + IL-17 + , CD4 + CD25 + Foxp3 + and pSTAT3 cells in spleens were determined using confocal immunostaining or flowcytometric analyses. Serum antibody levels and B cell-associated marker mRNAs were analyzed with ELISAs and qRT-PCR, respectively. CD4 + T cells and CD19 + B cells were purified from mice spleens for in vitro studies. Results: UA treatment significantly reduced the incidence and severity of CIA-induced arthritis, accompanied by decreased expression of proinflammatory cytokines (TNF-α, IL-1β, IL-6, IL-21 and IL-17) and oxidative stress markers (nitrotyrosine and iNOS) in arthritic joints. In CIA mice, UA treatment significantly decreased the number of Th17 cells, while increased the number of Treg cells in the spleens, which was consistent with decreased expression of pSTAT3, along with IL-17 and RORγt in the splenocytes. In addition, UA treatment significantly reduced the serum CII-specific IgG levels in CIA mice. The inhibitory effects of UA on Th17 cells were confirmed in an in vitro model of Th17 differentiation. Furthermore, UA dose-dependently suppressed the expression of B cell-associated markers Bcl-6, Blimp1 and AID mRNAs in purified CD19 + B cells pretreated with IL-21 or LPS in vitro. Conclusion: UA treatment significantly ameliorates CIA in mice via suppression of Th17 and differentiation. By targeting pathogenic Th17 cells and autoantibody production, UA may be useful for the treatment of autoimmune arthritis and other Th17-related diseases.

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Ursolic acid, a potential PPARγ agonist, suppresses ovalbumin-induced airway inflammation and Penh by down-regulating IL-5, IL-13, and IL-17 in a mouse model of allergic asthma

Cited by 94 publications

References 64 publications

Bronchodilatory effect of <i>Myxopyrum serratulum</i> in animal model

Bronchodilatory effect of <i>Myxopyrum serratulum</i> in animal model

Allergenicity and toxicology of inhaled silver nanoparticles in allergen-provocation mice models

Ursolic acid ameliorates autoimmune arthritis via suppression of Th17 and B cell differentiation

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