MATERIALS AND METHODS
MaterialsMaterials include the following: Cox-2 (human) inhibitor screening assay kit (Cayman Chemical); n-hexane, ethyl acetate, methanol, formic acid, 96% ethanol, all solvents are pro analyses grade (Merck, Darmstadt, Germany); distilled water; DPPH radical (2,2-diphenyl-1-phycrilhydrazyl) (Sigma Adrich); standard used for the TLC are quercetin, gallic acid, quinine, stigmasterol (Merck, Germany); spraying reagents used are FeCl 3 , AlCl 3 , Dragendorff, annisaldehyde, H 2 SO 4 (Merck, Germany).
EquipmentsLinomat (Camag Linomat 5), Chamber (Durant), Microscope binocular XSG, Spectrophotometer (Geneys 10 UV Scanning, 335903).