Uruburuella testudinis sp. nov., isolated from tortoise (Testudo)

Abstract

A polyphasic taxonomic analysis was carried out on 11 uncommon Gram-stain-negative, non-motile, catalase-and oxidase-positive, but indole-negative, bacterial strains isolated from tortoises. Phenotypically and genetically they represented a homogeneous group of organisms most closely related to, but distinct from, Uruburuella suis. In a reconstructed 16S rRNA gene tree they clustered on a monophyletic branch next to U. suis with gene similarities between strains of 99.5-100 %, and of up to 98.2 % with U. suis. DNA-DNA hybridization indicated the organisms represented a novel species with only 40 % DNA-DNA similarity with U. suis. Partial sequencing of rpoB resulted in two subclusters confirming the 16S rRNA gene phylogeny; both genes allowed clear separation and identification of the novel species. Furthermore, they could be unambiguously identified by matrix-assisted laser desorption ionization time-of-flight MS, where, again, they formed a highly homogeneous cluster separate from U. suis and other members of the family Neisseriaceae. The major fatty acids were C 16 : 0 and summed feature C 16 : 1 v7c/iso-C 15 : 0 2-OH. The DNA G+C content was 54.4 mol%. Based on phenotypic and genetic data we propose classifying these organisms as representatives of a novel species named Uruburuella testudinis sp. nov. The type strain is 07_OD624 T (5DSM 26510 T 5CCUG 63373 T ).

Vertebral fracture due to Actinobacillus pleuropneumoniae osteomyelitis in a weaner

“…The use of ribosomal RNA gene amplification and sequencing has been applied efficaciously for microbial diagnostics for many years in our laboratory [31][32][33][34][35][36][37]. These include several clinical cases and the use of embedded material of a paraffin block as successfully done in the present report confirming the potential of the approach for retrospective analyses.…”

Challenges in microbiological identification of aerobic bacteria isolated from the skin of reptiles

“…Exiguobacterium or Deinococcus have been previously isolated from reptiles: Uruburuella testudinis, which was isolated from a Greek tortoise and a Hermann's tortoise in this study, has also been described in tortoises of the genus Testudo before [37]. This bacterial species does not seem to be skin-specific and has been isolated from different organs, mainly from the pharynx [37].…”

“…Exiguobacterium or Deinococcus have been previously isolated from reptiles: Uruburuella testudinis, which was isolated from a Greek tortoise and a Hermann's tortoise in this study, has also been described in tortoises of the genus Testudo before [37]. This bacterial species does not seem to be skin-specific and has been isolated from different organs, mainly from the pharynx [37]. In previous studies, two of eleven chelonians infected with Uruburuella testudinis were described to be septicaemic, and the closely related species Uruburuella suis has been isolated from five pigs with pneumonia and pericarditis [38].…”

Paralysiella testudinis gen. nov., sp. nov., isolated from the cloaca of a toad-headed turtle (Mesoclemmys nasuta)

“…Fatty acid content (%) of strain 26B T and related species as indicated from 16S rRNA gene sequence data and/or ANI values Strains: 1, 26B T (this study); 2, Uruburuella suis 1258/02 T[15]; 3, Uruburuella testudinis 07_OD624 T[49]; 4, Alysiella filiformis IAM 14895 T[1]; 5, Alysiella crassa IAM 14969 T[1]; 6, Neisseria shayeganii 871 T[21]; 7, Neisseria animaloris LMG 23011 T[26].Colonies on 3.3×PYE agar are beige-pigmented and reach 5 mm in diameter after 4 days of incubation at 37 °C. Grows well at 20, 28 and 37 °C and weakly at 4 °C.…”