Usage Mitochondrial 16S rRNA Gene as Molecular Marker in Taxonomy of Cyprinin Fish Species (Cyprinidae: Teleostei)

Faddagh, Mustafa S.; Husain, Najah; Al-Badran, Adnan Issa

doi:10.4197/mar.23-1.3

Cited by 8 publications

(5 citation statements)

References 15 publications

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“…long in fish (Naock et al, 1996), which is highly conserved. The mitochondrial (16S) gene is very commonly used to examine the phylogenetic linkages of fishes at different taxonomic levels (Faddagh, et al, 2012). Segments of sequence of this region are efficiently used for constructing phylogenies (Singh et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Molecular Phylogenetic Linkage for Nile and Marine Puffer Fishes Using Mitochondrial DNA sequences of Cytochrome b and 16S rRNA

Marie¹,

Allam²

2019

Egypt. J. of Aquatic Biolo. and Fish.

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Section: Introductionmentioning

confidence: 99%

Molecular Phylogenetic Linkage for Nile and Marine Puffer Fishes Using Mitochondrial DNA sequences of Cytochrome b and 16S rRNA

Marie¹,

Allam²

2019

Egypt. J. of Aquatic Biolo. and Fish.

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“…These results agreed with Faddagh et al (2012a), who determined that BP has 89 % common ancestry. Faddagh et al (2012b) also determined that taxonomical status of some cyprinid species in Iraqi waters using the mitochondrial 16S rRNA gene: Barbus kersin (=L. kersin), B. grypus (=L.…”

Section: Molecular Studymentioning

confidence: 99%

Morphological and Molecular Studies of Kais Kingfish Cyprinion Kais Heckel, 1843 (Piscies, Cypriniformes, Cyprinidae) From the Middle of Iraq

Dyia Hadi,

Hassan Baraaj,

Hussain Ali

2023

Bull. Iraq nat. Hist. Mus.

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Cyprinidae species are the most abundant and widely distributed fish species in the inland waters of Iraq. Cyprinids are complex species, and it is difficult to identify them on the basis of morphology. Thus, the morphological characteristics must be achieved and confirmed by molecular analysis. Twenty specimens of Cyprinion kais Heckel, 1843 (Piscies, Cypriniformes, Cyprinidae) were collected from two localities at Tigris River in the middle of Iraq: five specimens from Al-Tharthar Lake, Saladin Province, and 15 specimens from Al- Zubaydiyah sub-district, Wasit Province. The DNA sequences of C. kais were done using the mitochondrial DNA cytochrome b (cytb) gene. After analysis, the sequences were compared with sequences of other fish genera and species available in GenBank. The barcoding result (DNA sequencing) showed that it fit with C. kais. We conclude the results of the current study confirm that the validity of the morphological diagnosis of C. kais, using DNA sequencing analysis.

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“…Mitochondrial 16S rRNA gene also contains sufficient polymorphisms to unambiguously discriminate most species due to a high number of insertions and deletions in non-peptide coding DNA. In addition, the 16S rRNA gene is ubiquitous (Faddagh et al, 2012;Srinivasan et al, 2015;Satoh et al, 2016;Hossain et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

GENETIC CHARACTERIZATION OF DWARF SNAKEHEAD, Channa gachua (Hamilton, 1822), FROM TWO POPULATIONS BASED ON 16S rRNA GENE

Pinasti¹,

Ilmi²,

Suryani³

2021

Indonesian Fisheries Research Journal

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Indonesia is one of the countries with high biodiversity of freshwater fishes. One freshwater fish with a wide distribution in Asia with high economic potency for ornamental fish is dwarf snakehead. However, research on genetic characterization of dwarf snakehead from Indonesia is very limited. Therefore, this research aimed to compare genetic characterization of the dwarf snakehead from rice field irrigation at Donomulyo Village (Malang, East Java) and from Keji River (Magelang, Central Java). This study used a PCR method with universal primers: 16Sar and 16Sbr. The data obtained in this study were then analyzed using DNASTAR, BLAST, Mesquite, MEGA, DnaSP, and NETWORK. The results revealed the genetic distance between dwarf snakehead from rice field irrigation at Donomulyo Village and from Keji River was 1.36%. The divergence of GC content, haplotype number, variables sites, haplotype diversity, and nucleotide diversity in both populations exist. The results of this study are expected to arrange 16S mitochondrial DNA Library of dwarf snakehead from Indonesia which is useful for a reference in the conservation and utilization and management of dwarf snakehead in their habitat.

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Usage Mitochondrial 16S rRNA Gene as Molecular Marker in Taxonomy of Cyprinin Fish Species (Cyprinidae: Teleostei)

Cited by 8 publications

References 15 publications

Molecular Phylogenetic Linkage for Nile and Marine Puffer Fishes Using Mitochondrial DNA sequences of Cytochrome b and 16S rRNA

Molecular Phylogenetic Linkage for Nile and Marine Puffer Fishes Using Mitochondrial DNA sequences of Cytochrome b and 16S rRNA

Morphological and Molecular Studies of Kais Kingfish Cyprinion Kais Heckel, 1843 (Piscies, Cypriniformes, Cyprinidae) From the Middle of Iraq

GENETIC CHARACTERIZATION OF DWARF SNAKEHEAD, Channa gachua (Hamilton, 1822), FROM TWO POPULATIONS BASED ON 16S rRNA GENE

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