Usage of Conventional PCR Technology for the Detection of HLA-B27 Allele: A Significant Molecular Marker of Ankylosing Spondylitis

Abstract

Ankylosing spondylitis is a chronic inflammatory disease that has been linked to the human leukocyte antigen class I allele HLA-B27. More than 90 % of patients with ankylosing spondylitis possess the HLA-B27 allele, but only 1 % of people with HLA-B27 develop the disease. Ankylosing spondylitis predominately affects young males. The present study was planned to find out the involvement of HLA-B27 specific allele in relation to age and sex in symptomatic suspected patients of ankylosing spondylitis using conventional PCR technology. Forty symptomatic patients of ankylosing spondylitis were included in the present study. SSP-PCR technique was used to detect the HLA-B27 specific allele. This study showed (out of 40 symptomatic suspected cases of ankylosing spondylitis only 12 patients were detected positive with HLA-B27 allele, while remaining 28 were negative) that the positivity rate for ankylosing spondylitis with HLA-B27 allele is low. Furthermore, it was observed that the males above 50 years are more prone to develop AS with HLA-B27 specific allele. It could be concluded that the conventional PCR technology is a rapid, sensitive, and confirmatory method for the diagnosis of ankylosing spondylitis.

Association of rs11209032 and rs1004819 Polymorphisms in Interleukin-23 Receptor Gene With Ankylosing Spondylitis

“…In two different studies held in Chinese population, Yi et al 20 reported a percentage of 93.3% of HLA-B27 positivity in 360 AS patients, while Xiong et al 21 reported a percentage of 90.7% in 350 AS patients. On the other hand, in the study conducted in India with a smaller sample size (n=40), Sharma et al 22 found a very low frequency of HLA-B27 allele positivity (30%) in AS patients. Similar to our findings, the HLA-B27 allele frequency in AS patients were found as 62.5% in Serbia, 69% in Qatar, and 71.7% in Iran populations.…”

HLA‐B27 testing: A journey from flow cytometry to molecular subtyping

“…13 Serological techniques for HLA antigen detection include classic microcytotoxicity and flow cytometry. 19 Originally described by Terasaki and McClelland (1964), microcytotoxicity was long-established method for HLA typing, but cross-reactivity induced HLA-typing errors and labor intensiveness limited its further use. 20 Flow cytometry is a rapid and relatively inexpensive method for preliminary screening of HLA antigen.…”

“…Excluding HLA‐B27 virtually excludes the possibility of ankylosing spondilytis . Serological techniques for HLA antigen detection include classic microcytotoxicity and flow cytometry . Originally described by Terasaki and McClelland (1964), microcytotoxicity was long‐established method for HLA typing, but cross‐reactivity induced HLA‐typing errors and labor intensiveness limited its further use .…”

Hip involvement in children with enthesitis related arthritis (ERA) is associated with poor outcomes in adulthood

“…DNA was extracted by salting out technique. The presence of HLA B27 was analyzed using ARMS-PCR by the previously described method [16]. CRP was measured by Nephelometry.…”