Usage of  Leptospira  spp. local strains as antigens increases the sensitivity of the serodiagnosis of bovine leptospirosis

Pinto, Priscila S.; Loureiro, Ana Paula; Penna, Bruno; Lilenbaum, Walter

doi:10.1016/j.actatropica.2015.05.008

Cited by 28 publications

(19 citation statements)

References 26 publications

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“…Serjoe was the predominant serogroup in cattle, being frequently mentioned in several epidemiological surveys in Brazil and in other parts of the world (MARTINS; LILENBAUM, 2013;MENEGAS et al, 2013;PINTO et al, 2015PINTO et al, , 2016TAGLIABUE et al, 2016;CORREIA et al, 2017;CAMPOS et al, 2017). Bovine animals are recognized maintenance hosts of this serogroup, responsible for the development of chronic disease and subclinical persistent reproductive tract infection.…”

Section: Resultsmentioning

confidence: 99%

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Seroprevalence and predominant serogroups of Leptospira sp. in serological tests of ruminants in northeastern Brazil

Pimenta

Bezerra

Morais

et al. 2019

SCA

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The objective of this study was to determine the seroprevalence of leptospirosis and the predominant serogroups in serological tests of ruminants performed at the Transmissible Diseases Laboratory of the Federal University of Campina Grande, Patos, Paraíba, northeastern Brazil, between 2010 and 2017. This research included the records of serological tests for leptospirosis from 5594 animals, including 1527 cattle, 1761 goats, 2170 sheep, and 136 buffaloes from four Brazilian states (Paraíba, Pernambuco, Maranhão, and Rio Grande do Norte). Of the 5594 serum samples, 662 were serologically positive, corresponding to a frequency of 11.8%. Serjoe (30.6%), Autumnalis (13.6%), and Icterohaemorrhagiae (11.3%) were the most frequent subgroups among all species. The individual frequencies in cattle, goats, sheep, and buffaloes were 20% (306/1527), 8.3% (147/1761), 7.9% (171/2170), and 27.9% (38/136), respectively, with titers ranging from 1:100 to 1:3200. The most frequent serogroups according to animal species were as follows: Serjoe predominated in cattle (62%), followed by Icterohaemorrhagiae (12.5%) and Tarassovi (6.6%); Autumnalis was the most frequent in goats and sheep (29.4% and 26.9%, respectively), followed by Seramanga (12.5%) in goats and Icterohaemmorrhagiae (13.5%) in sheep; and Australis predominated in buffaloes (39.5%), followed by Pomona (31.6%) and Canicola (21.1%). Leptospira sp. infection, determined by serology, is widespread in ruminants (cattle, goats, sheep, and buffaloes) in northeastern Brazil, suggesting the existence of alternative transmission routes that are less dependent on environmental factors. Identification of the most frequent serogroups highlights the need to improve sanitary conditions, and implement efficient and targeted control measures directed at the primary infection sources.

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Section: Resultsmentioning

confidence: 99%

“…The World Organization for Animal Health (OIE) recommends serological diagnosis using the microscopic agglutination test (MAT), particularly for herd diagnosis of production animals (SUEPAUL et al, 2011;PINTO et al, 2015). Thus, seroepidemiological surveys are necessary to monitor and control leptospirosis in a given region.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence and predominant serogroups of Leptospira sp. in serological tests of ruminants in northeastern Brazil

Pimenta

Bezerra

Morais

et al. 2019

SCA

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“…This suggests a possible interface between wild and rural cycles, what remains to be better understood. In this context, an outstanding finding of the present study was that sample 19524, which was obtained from a N. squamipesi, showed 100% similarity to the L. santarosai U164/2013 strain, which was isolated from bovine urine in this same region (Pinto, Loureiro, Penna, & Lilenbaum, ). This finding indicates a possible circulation of strains between wildlife and livestock, what has already been demonstrated for Mozdok strains on Spain (Arent et al., ).…”

Section: Discussionmentioning

confidence: 54%

PathogenicLeptospiraspecies are widely disseminated among small mammals in Atlantic Forest biome

Vieira

D’Andrea

Vilela

et al. 2019

Transbound Emerg Dis

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Leptospirosis is a common worldwide bacterial zoonosis and has been studied in One Health approaches. Small mammals are described as the most important maintenance reservoirs of several pathogens in nature, including leptospires. The aim of this study was to identify infection by leptospires among small mammals on the Atlantic forest biome and evaluate their potential as carriers of these spirochetes. A total of 153 small mammals belonging to orders Rodentia and Didelphimorphia (distributed on 17 genera and 22 species) were captured. Blood and kidney samples were collected from animals and a conventional PCR targeted on lipL32 gene was conducted on renal tissues. Species identification was performed in eight samples by sequencing of rrs gene. A total of 28% of the animals presented lipL32 PCR‐positive, and four pathogenic Leptospira species (L. interrogans, L. borgpetersenii, L. santarosai and L. noguchii) were identified. This study highlights the role of small mammals as carriers of leptospires on the Atlantic Forest representing a potential source of pathogenic Leptospira spp infection for both humans and domestic animals.

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“…serovar Hardjo infected animals were detected using the strains Lagoa and Bolivia, isolated in Minas Gerais (Chiareli et al, 2012), which supports the use of local isolates of Leptospira spp. to increase the sensitivity of the diagnosis (Lage et al, 2007;Pinto, Loureiro, Penna, & Lilenbaum, 2015). Moreover, these results confirm that the most important Leptospira spp.…”

Section: Discussionmentioning

confidence: 55%

“…for cattle is serovar Hardjo, for which cattle is the main reservoir (Lage et al, 2007). Interestingly, our serological survey also showed that L. interrogans serovar Autumnalis, a serovar that has only been associated with incidental cattle infections (Pinto et al, 2015), was associated with abortion. L. interrogans serovar Autumnalis has as reservoirs rodents and dogs (Petrakovsky, Bianchi, Fisun, Nájera-Aguilar, & Pereira, 2014), animals that usually have access to farms and cattle, and thereby could play a role in the epidemiology of bovine leptospirosis.…”

Section: Discussionmentioning

confidence: 58%

Seroprevalence and intercurrence of reproductive pathogens in cattle from family farms in North of Minas Gerais, Brazil

Haas

Barbieri

Freitas

et al. 2020

SCA

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The aims of the present study were: to estimate the seroprevalence of Bluetongue vírus (BTV), bovine alphaherpesvirus 1 (BoHV-1), bovine viral diarrhea virus (BVDV), Brucella abortus and Leptospira spp. in cattle from family farms in North of Minas Gerais; to determine the intercurrence and association among these pathogens; and to assess the possible associated factors to seropositive herds and animals. For this, 476 cows from 46 farms were sampled and evaluated serologically. The seroprevalence in herds and cattle was, respectively, for BTV 100% and 52.0%, for BoHV-1 95.7% and 48.6%, for BVDV 78.3% and 46.1%, for Leptospira spp. 76.1% and 29.1%, and for B. abortus was 0% in herd and cattle. More than 65% of the herds was simultaneously seropositive for BTV, BoHV-1, BVDV and Leptospira spp. Seropositivity for BoHV-1, Leptospira spp. serovar Autumnalis and serovar Hardjoprajitno were associated with abortion, whereas seropositivity for BVDV was associated with BoHV-1. Moreover, association among seropositivity for BVDV, BoHV-1 and Leptospira spp. was also observed. In conclusion, BTV, BoHV-1, BVDV and Leptospira spp. are highly seroprevalent and occurred simultaneously in cattle from family farms in Minas Gerais, indicating the need for the implementation of control measures to avoid economic losses related to these diseases.

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Usage of Leptospira spp. local strains as antigens increases the sensitivity of the serodiagnosis of bovine leptospirosis

Cited by 28 publications

References 26 publications

Seroprevalence and predominant serogroups of Leptospira sp. in serological tests of ruminants in northeastern Brazil

Seroprevalence and predominant serogroups of Leptospira sp. in serological tests of ruminants in northeastern Brazil

PathogenicLeptospiraspecies are widely disseminated among small mammals in Atlantic Forest biome

Seroprevalence and intercurrence of reproductive pathogens in cattle from family farms in North of Minas Gerais, Brazil

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