It is known that gloss has a significant impact on colour measurement. The UV varnish used for surface finishing, spot effects, as well as special effects, such as 3D effects and formation of Braille, has a pronounced gloss effect. In this work, a comparison of the spectrophotometer measurement geometry influence on the colour measurement of an unprinted PVC sample covered with UV varnish was done. UV varnish was applied successively in layers, and patches with different number of varnish layers, from 1 to 12, were formed. Two spectrophotometers with different measurement geometries were used for the measurement: Konica Minolta CM-2600d with d/8 measurement geometry, with spectral reflection included and excluded, and different measurement aperture 3 and 8 mm, and X-Rite eXact with 45/0 measurement geometry. By comparing the measurements, it was estab-lished that there is a significant difference in the measured values between different measuring device geometries on the given samples, and that the number of UV varnish layers has a significant influence on this differenc