Use of 3D-printed polylactic acid/bioceramic composite scaffolds for bone tissue engineering in preclinical in vivo studies: A systematic review

Fernández, Iván Alonso; Haugen, Håvard Jostein; López-Peña, Mónica; González‐Cantalapiedra, Antonio; Muñoz, Fernando

doi:10.1016/j.actbio.2023.07.013

Cited by 41 publications

(8 citation statements)

References 168 publications

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“…Given that research indicates PLLA to be a porous scaffold [ 24 ], we hypothesize that the structure of the GO/PLLA scaffold is influenced by the inherent properties of PLLA. These pores play a crucial role in allowing the cells to expand, establish connections, and occupy the spaces within the scaffold [ 25 ]. As expected, the results of filamentous actin staining, CCK-8, and live/dead cell viability assay showed that GO/PLLA scaffolds did not impede cell adhesion and proliferation, indicating that GO/PLLA scaffolds possessed good mechanical properties and cytocompatibility for DPSCs, which agrees well with results from previous studies on GO/PLLA composites [ 16 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of graphene oxide/ poly-L-lactic acid composite scaffold on the biological properties of human dental pulp stem cells

Qiu,

Lin,

Zou

et al. 2024

BMC Oral Health

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Background Tissue engineering has attracted recent attention as a promising bone repair and reconstruction approach. Dental pulp stem cells (DPSCs) are pluripotent and can differentiate into bone cells with the correct environment and substrate. Therefore, suitable scaffold materials are essential for fabricating functional three-dimensional (3D) tissue and tissue regeneration. Composite scaffolds consisting of biodegradable polymers are very promising constructs. This study aims to verify the biological function of human DPSCs seeded onto composite scaffolds based on graphene oxide (GO) and poly-L-lactic acid (PLLA). Methods The surface morphology was observed under scanning electron microscopy (SEM). Chemical composition was evaluated with Fourier transform infrared (FTIR) spectroscopy. The biocompatibility of GO/PLLA scaffolds was assessed using phalloidin staining of cytoskeletal actin filaments, live/dead staining, and a CCK-8 assay. The effect of GO/PLLA scaffolds on cell osteogenic differentiation was detected through ALP staining, ALP activity assays, and alizarin red S staining, complemented by quantitative real-time PCR (qRT-PCR) analysis. Results Our data showed that GO and PLLA are successfully integrated and the GO/PLLA scaffolds exhibit favorable bioactivity and biocompatibility towards DPSCs. Additionally, it was observed that the 0.15% GO/PLLA scaffold group promoted DPSC proliferation and osteogenic differentiation by forming more calcium nodules, showing a higher intensity of ALP staining and ALP activity, and enhancing the expression levels of differentiation marker genes RUNX2 and COL1. Conclusions These results demonstrate that the GO/PLLA scaffold is a feasible composite material suitable for cell culture and holds promising applications for oral bone tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Effect of graphene oxide/ poly-L-lactic acid composite scaffold on the biological properties of human dental pulp stem cells

Qiu,

Lin,

Zou

et al. 2024

BMC Oral Health

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“…Considering that a single material may not entirely meet the requirements of bone scaffolds, the use of multiple materials in a composite can compensate for the limitations of individual materials. 31 For instance, ceramic materials often face challenges such as high brittleness, slow degradation, and difficulties in constructing a porous structure. However, when combined with polymer materials, these drawbacks can be mitigated, and the ceramic component can enhance the composite material's osteoconductive properties.…”

Section: Bone Tissue Engineering Scaffoldmentioning

confidence: 99%

Construction of antibacterial bone implants and their application in bone regeneration

Feng,

He,

et al. 2024

Mater. Horiz.

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“…[8,[43][44][45][46]. Critical size defect are defined as the smallest wound that does not heal spontaneously over a long period of time, so they are commonly performed to evaluate scaffold's bone healing properties [8,27].…”

Section: Animal Modelmentioning

confidence: 99%

“…Which optimizes the biocompatibility of the polymeric materials but maintains adequate mechanical properties and avoids the bri le behavior of the bioceramics [14]. Furthermore, bioceramics also block the acidic environment originating from PLA's degradation and increase its hydrophilicity and degradation rate [27].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Bone Healing in Critical-Sized Rabbit Femoral Defects: Impact of Helical and Alternate Scaffold Architectures.

Alonso Fernández,

Haugen,

Nogueira

et al. 2024

Preprint

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This study investigates the effect of scaffold architecture on bone regeneration, focusing on 3D-printed polylactic acid-bioceramic calcium phosphate (PLA-bioCaP) composite scaffolds in rabbit femoral condyle critical defects. To assess their influence on bone healing and scaffold performance, we explored two distinct scaffold designs, alternate and helical laydown patterns. The scaffolds were meticulously characterized for pore size, strut thickness, porosity, pore accessibility, and mechanical properties. The in vivo efficacy of these scaffolds was evaluated using a rabbit femoral condyle critical defect model. Our findings indicate that both scaffold architectures are biocompatible and support bone formation. The helical scaffolds, characterized by larger pore sizes and higher porosity, demonstrated significantly greater bone regeneration than the alternate structures. However, their lower mechanical strength presented limitations for use in load-bearing sites. This study highlights the importance of scaffold architecture in bone tissue engineering and underscores the need to balance porosity and mechanical strength for optimal bone regeneration. The findings provide crucial insights for the design of 3D- printed scaffolds in clinical applications, particularly in the context of critical-sized bone defects. The study also outlines potential areas for future research, including exploring hybrid scaffold designs to achieve optimal porosity without compromising mechanical integrity.

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Use of 3D-printed polylactic acid/bioceramic composite scaffolds for bone tissue engineering in preclinical in vivo studies: A systematic review

Cited by 41 publications

References 168 publications

Effect of graphene oxide/ poly-L-lactic acid composite scaffold on the biological properties of human dental pulp stem cells

Effect of graphene oxide/ poly-L-lactic acid composite scaffold on the biological properties of human dental pulp stem cells

Construction of antibacterial bone implants and their application in bone regeneration

Enhanced Bone Healing in Critical-Sized Rabbit Femoral Defects: Impact of Helical and Alternate Scaffold Architectures.

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