Use of a borescope to determine the sex and egg maturity stage of sturgeons and the effect of borescope use on reproductive structures

Kynard, Boyd; Kieffer, Micah

doi:10.1046/j.1439-0426.2002.00388.x

Cited by 49 publications

(45 citation statements)

References 5 publications

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“…Because there is no external sexual dimorphism in the some sturgeon, for example, the Russian sturgeon, the development of internal gonads is the only biomarker for sex identification. So far, there are four different internal examination methods for sex identification in sturgeons, including invasive surgery (14,17,27), endoscopy (37), ultrasound (62), and measurement of biochemistry and hormone levels in the blood (4,9,21,47,50). The sex of sturgeons can be confirmed via an endoscopic system with very high accuracy (95%) (29).…”

mentioning

confidence: 99%

Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads

Chen

Xia²,

Shao

et al. 2016

Physiological Genomics

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Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads. Physiol Genomics 48: 464 -476, 2016. First published May 6, 2016 doi:10.1152/physiolgenomics.00113.2015.-As the Russian sturgeon (Acipenser gueldenstaedtii) is an important food and is the main source of caviar, it is necessary to discover the genes associated with its sex differentiation. However, the complicated life and maturity cycles of the Russian sturgeon restrict the accurate identification of sex in early development. To generate a first look at specific sex-related genes, we sequenced the transcriptome of gonads in different development stages (1, 2, and 5 yr old stages) with next-generation RNA sequencing. We generated Ͼ60 million raw reads, and the filtered reads were assembled into 263,341 contigs, which produced 38,505 unigenes. Genes involved in signal transduction mechanisms were the most abundant, suggesting that development of sturgeon gonads is under control of signal transduction mechanisms. Differentially expressed gene analysis suggests that more genes for protein synthesis, cytochrome c oxidase subunits, and ribosomal proteins were expressed in female gonads than in male. Meanwhile, male gonads expressed more transposable element transposase, reverse transcriptase, and transposase-related genes than female. In total, 342, 782, and 7,845 genes were detected in intersex, male, and female transcriptomes, respectively. The female gonad expressed more genes than the male gonad, and more genes were involved in female gonadal development. Genes (sox9, foxl2) are differentially expressed in different sexes and may be important sex-related genes in Russian sturgeon. Sox9 genes are responsible for the development of male gonads and foxl2 for female gonads.

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confidence: 99%

Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads

Chen

Xia²,

Shao

et al. 2016

Physiological Genomics

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“…Ortenburger et al (1996), realizando a sexagem de Arctic charr, observaram resultados semelhante ao descrito neste trabalho. Dos 60 peixes examinados utilizando a endoscopia via poro urogenital e confirmado via histologia das gônadas, foi observada correta classificação do sexo em 95% (n = 57) dos animais (Tabela 1), resultado semelhante, utilizando a mesma técnica, foi encontrado em outras espécies de peixes por Ortenburger et al (1996) e Kynard & Kieffer (2002).…”

Section: Resultsunclassified

“…A técnica apresenta algumas características que o torna confiável para utilização na sexagem, como: (1) não causar lesão às estruturas reprodutivas dos peixes; (2) utilização em campo em alguns minutos; (3) utilização em qualquer condição de tempo; (4) possibilidade de distinguir oócitos que irão ficar maduros na próxima estação reprodutiva; (5) pode ser facilmente ensinada a técnicos de campo dentre outras (Kynard & Kieffer, 2002).…”

Section: Introductionunclassified

Determinação do sexo em catfish de canal (Ictalurus punctatus) utilizando endoscopia

Lima¹,

Murgas²,

Felizardo³

et al. 2011

Rev. Ceres

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Sex determination in channel catfish (Ictalurus punctatus) using endoscopySex identification in fish is an important practice for the development of broodstock management strategies. Endoscopy can be used for sex identification, although in fish, it is still restricted. Therefore, the objective of this study was to validate the use of rigid endoscope via the urogenital pore to determine the sex of the catfish (Ictalurus punctatus afinesque, 1818). The equipment used was a Multi-Purpose Rigid ™ 30º endoscope, with 2.7 mm in diameter and 18 cm in length. We examined 60 fish weighing between 86 and 1032 grams, in which the endoscope was introduced via the genital pore and then immediately euthanized to confirm the sex. Gonadal tissue samples were collected for histological assessment of maturation stage and sex confirmation. The results showed that of the 60 fish examined, 57 had positive sex confirmation by endoscopy, corresponding to 95% of the sample. The results show that it is feasible the use of endoscopy for sex determination in catfish via genital pore.

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“…In most cultivated fish species, phenotypic sex is easily identified by observation of specific external characters such as scale coloration and the presence of genital papilla during early sexual maturation. Methods currently used for sexual identification of fish species include intra-ovarian biopsy, ultrasonography and endoscopy (Ortenburger et al 1996;Kynard 2002;Wildhaber et al 2005;Bryan et al 2007;Hurvitz et al 2007). For some fish species, DNA markers are available for sexual identification at the genetic level and in these cases the differentiation is based on distinguished gender-specific band patterns.…”

Section: Introductionmentioning

confidence: 99%

Sex determination in the Giant fish of Amazon Basin, Arapaima gigas (Osteoglossiformes, Arapaimatidae), using laparoscopy

Carreiro

Furtado-Neto

Mesquita³

et al. 2011

Acta Amaz.

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The Giant of Amazon basin, pirarucu, Arapaima gigas, is the largest scaled freshwater fish in the world. Pirarucu cultivation has recently started, driven by the decline in natural populations and high market value. Currently, there are no reliable methods for sexual differentiation in this species other than direct examination of gonads, which requires dissection of specimens. A non-lethal and less invasive method for sexual identification is highly desirable in order to properly group broodstock for mating and offspring production. We utilized laparoscopic examination in anesthetized pirarucu to differentiate between male and female individuals. This method allowed for the observation and differentiation of the reproductive organs within an individual. Our results suggest that laparoscopy is an efficient method for sex differentiation in pirarucu causing minimal stress to the fish. Determinação do sexo no peixe gigante da Bacia Amazônica, Arapaima gigas (Osteoglossiformes, Arapaimatidae), usando laparoscopia.O gigante da bacia amazônica, pirarucu, Arapaima gigas, é o maior peixe de escamas de água doce do mundo. O seu cultivo teve início recentemente motivado pelo declínio de suas populações naturais em virtude do alto valor de sua carne no mercado. Não existem métodos para a diferenciação sexual nesta espécie, o único método é o exame direto das gônadas, o qual requer a dissecação do espécime. Um método para a identificação sexual é altamente desejável, a fim de selecionar grupos de reprodutores para o acasalamento e, portanto, a produção de progênie. A fim de diferenciar reprodutores machos de fêmeas, foi utilizado o exame laparoscópico em animais previamente anestesiados. Este método permitiu a observação dos órgãos abdominais, incluindo gônadas, com grande precisão. Os resultados mostram que a laparoscopia é um método eficiente para a diferenciação do sexo em pirarucu e causam o mínimo de stress para os animais.

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Use of a borescope to determine the sex and egg maturity stage of sturgeons and the effect of borescope use on reproductive structures

Cited by 49 publications

References 5 publications

Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads

Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads

Determinação do sexo em catfish de canal (Ictalurus punctatus) utilizando endoscopia

Sex determination in the Giant fish of Amazon Basin, Arapaima gigas (Osteoglossiformes, Arapaimatidae), using laparoscopy

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