Use of a Centrifugal Analyzer for a Chromogenic Prothrombin Time, a Chromogenic Activated Partial Thromboplastin Time and a Kinetic Fibrinogen Assay in a Routine Hospital Laboratory

Metz, M. de; Wersch, J. W. J. Van

doi:10.1159/000215752

Cited by 3 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A great variety of methods, reagents and analysers are used for fibrinogen assay (17,18), and laboratories focus on ease of use, throughput and cost. The widespread availability of the PT-Fg (9,(19)(20)(21)(22)(23)(24)(25) has raised issues about the variability and clinical utility of different fibrinogen assays (2,(5)(6)(7)(8)26). Most studies suggest disparity between PT-Fg and Clauss assays, but have been limited to particular reagent/analyser combinations; there is little information concerning reagent, analyser and calibrant related discrepancies within each method.…”

Section: Discussionmentioning

confidence: 99%

A Performance Evaluation of Commercial Fibrinogen Reference Preparations and Assays for Clauss and PT-derived Fibrinogen

Mackie

Lawrie²,

Kitchen³

et al. 2002

Thromb Haemost

View full text Add to dashboard Cite

SummaryThe wide availability of fibrinogen estimations based on the prothrombin time (PT-Fg) has caused concern about the variability and clinical utility of fibrinogen assays. In a multi-centre study, we investigated fibrinogen assays using various reagents and analysers. Clauss assays generally gave good agreement, although one reagent gave 15-30% higher values in DIC and thrombolysis. Two commercial reference preparations had much lower potencies than the manufacturers declared, and plasma turbidity influenced parallelism in some Clauss assays. PT-Fg assays gave higher values than Clauss and showed calibrant dependent effects, the degree of disparity correlating with calibrant and test sample turbidity. Analyser and thromboplastin dependent differences were noted. The relationship between Clauss and PT-Fg assays was sigmoid, and the plateau of maximal PT-Fg differed by about 2 g/l between reagents. ELISA and immunonephelometric assays correlated well, but with a high degree of scatter. Antigen levels were higher than Clauss, but slightly lower than PT-Fg assays, which appeared to be influenced by degraded fibrinogen. Clauss assays are generally reproducible between centres, analysers and reagents, but PT-Fg assays are not reliable in clinical settings.

show abstract

Section: Discussionmentioning

confidence: 99%

A Performance Evaluation of Commercial Fibrinogen Reference Preparations and Assays for Clauss and PT-derived Fibrinogen

Mackie

Lawrie²,

Kitchen³

et al. 2002

Thromb Haemost

View full text Add to dashboard Cite

show abstract

“…Fibrinogen was determined according to the Clauss method (intraassay CV 3.2%; (35)). Reference values, determined in plasma from 50 healthy volunteers ranged from 2.0 to 4.0 g/1.…”

Section: Laboratory Proceduresmentioning

confidence: 99%

Regular Physical Activity and Changes in Risk Factors for Coronary Heart Disease: A Nine Months Prospective Study

Ponjee¹,

Janssen²,

Hermans³

et al. 1996

CCLM

View full text Add to dashboard Cite

This study reports the non-acute effects of a long-term training programme of increasing intensity on some cardiovascular risk factors and the interrelation between these risk factors. Twenty sedentary men and 14 sedentary women were trained 3 to 4 times a week for nine months. After 36 weeks all individuals ran a half marathon run. The W max , weight, body mass index, systolic and diastolic blood pressure were recorded. The concentrations of fibrinogen, tissue plasminogen activator, plasminogen activator inhibitor, triacylglycerols, total cholesterol, LDL cholesterol, HDL cholesterol and lipoprotein(a) were measured. The training programme induced a median increase in W raax of 12% in the male group (from 226 to 251.5 Watt) and of 18% in the female group (from 160 to 188.5 Watt). These increases in W max did not correlate with any other property under investigation in this study. Blood pressure was not altered, but body weight and body mass index were significantly decreased in the male group (from 74.6 to 72.2 kg and from 23.1 to 22.0 kg/m 2 , respectively) at the end of the training programme and decreased non-significantly in the female group (from 63.0 to 60.7 kg and from 21.6 to 21.5 kg/m 2 , respectively). In the male group total cholesterol, low density lipoprotein cholesterol and triacylglycerols decreased significantly under the influence of the training sessions. Furthermore, in both groups, a great decrease in plasma plasminogen activator inhibitor concentrations was noticed: in men from 22.5 · 10 3 AU/1 to 4.5 · 10 3 AU/1 and in women from 18.7 · 10 3 AU/1 to 5.1 · 10 3 AU/1. However, the changes in the lipid and fibrinolytic quantities were not correlated with each other. Initial total cholesterol, LDL cholesterol and triacylglycerol levels correlated significantly with systolic blood pressure, while diastolic pressure was correlated to tissue plasminogen activator. Since tissue plasminogen activator also was significantly related to triacylglycerols, a trias existed between primary risk factors like blood pressure, lipid levels and fibrinolysis. In contrast, the changes in these properties under the influence of physical training were not interrelated. Median serum lipoprotein(a) concentrations were significantly increased in both men and women five days before the half marathon run: from 32 mg/1 to 39 mg/1 in men, and from 65 mg/1 to 125.5 mg/1 in women. Concomitantly, median fibrinogen concentrations were significantly elevated in men (from 2.32 g/1 to 3.10 g/1) and non-significantly in women (from 2.62 g/1 to 2.93 g/1), although no correlation existed between the changes in these properties.In conclusion, the nine months exercise programme increased the aerobic fitness in both men and women as indicated by the W max increase. This improvement coincided but was not correlated with beneficial changes in several anthropometric, lipid and fibrinolytic properties. Improvement in the risk factors under investigation was more pronounced in men than in women. The changes in lipid and haemostasis propert...

show abstract

“…; Die Kontrollplasmen PreciCIot® l/l l und PreciChrom® Fib i l/l l wurden in 17fach-Bestimmung in der Serie und an . 10 (13). Die Verlängerung der Gerinnungszeiten durch erhöhte Fibrin(ogen)-Spaltprodukte im koagulometrischen-, nicht jedoch in photometrischen Test stellt eine weitere Ursache aufgetretener Divergenzen dar.…”

Section: C) Methodenvergleichunclassified

“…In Übereinstimmung mit anderen Autoren (7,8,13,14) belegt die gute Übereinstimmung der TPZ-und aPTTErgebnisse mittels koagulometrischen und photometrischen Techniken bei einem heterogenen Kollektiv von 193 Patienten in dieser Untersuchung die Eignung von Thromboquant PT und Thromboquant aPTT zur Überwa-chung der Antikoagulantientherapie und als Suchteste auf Störungen des exogenen und endogenen Gerinnungssystems.…”

Section: Methodeunclassified

Applikation photometrischer Tests zur Bestimmung von Thromboplastinzeit (TPZ), aktivierter partieller Thromboplastinzeit (aPTT) und Fibrinogen an den Analysenautomaten Gem-Profiler

Ratge¹,

Wisser²

1988

LaboratoriumsMedizin

View full text Add to dashboard Cite

The application ofthe routine coagulation parameters thromboplastin time (TPZ), activated partial thromboplastin time (aPTT) and fibrinogen to the Gern-ProfHer centrifugalanalyzer ispresented. Instead of detection of the first fibrin Strands during clotting äs a measurement point, the photometric TPZ and aPTT methods relies on the measurement of the colored product p-nitroanilin at 405 nm. This colored product is cleaved out of the chromogenic Substrate by thrombin activated through the coagulation cascade. The kinetic assay for fibrinogen is based on aggregation of des-A-fibrin to fibrin S following proteolytic cleavage of fibrinogen with a solution of the thrombin-like snake venom batroxobin. Clotting velocity is recorded turbidimetrically at340nm, For the photometric coagulation tests coefficients of Variation from 0.7 to 4.8% within run and from 1.5 to 6.1% from day to day in control samples in the normal and abnormal ränge are comparable to that of mechanised assays of clinical chemistry. The correlations between the photometric and the coagulometric methods in a heterogenous patient group showed good agreements of the methods (r = 0.91-0.97). Lipemic specimens, hemolysis and bilirubin do notinterfere with fibrinogen determinations whereas bilirubin levels higher than 400 / Interrupt the measurement of TPZ and aPTT. The advantages of the photometric TPZ-and aPTTmethods are: fibrinogen-independent measurement, very good precision, easy performänce f 1 Electro Nucleonics International, Stuttgart. 232Lab.med. 12: 232 (1988) Brought to you by | University of Queensland -UQ Library Authenticated Download Date | 7/13/15 2:11 AM standardization and automatisation (sample throughput of 120-160/h). The mean features of the kinetic turbidimetric method for determination offunctional fibrinogen in comparison to the Clauss-technique are the broad measuring ränge without necessary sample dilutions, the better reliability and the Iower interference by fibrin/fibrinogen degradation products.

show abstract

Use of a Centrifugal Analyzer for a Chromogenic Prothrombin Time, a Chromogenic Activated Partial Thromboplastin Time and a Kinetic Fibrinogen Assay in a Routine Hospital Laboratory

Cited by 3 publications

References 6 publications

A Performance Evaluation of Commercial Fibrinogen Reference Preparations and Assays for Clauss and PT-derived Fibrinogen

A Performance Evaluation of Commercial Fibrinogen Reference Preparations and Assays for Clauss and PT-derived Fibrinogen

Regular Physical Activity and Changes in Risk Factors for Coronary Heart Disease: A Nine Months Prospective Study

Applikation photometrischer Tests zur Bestimmung von Thromboplastinzeit (TPZ), aktivierter partieller Thromboplastinzeit (aPTT) und Fibrinogen an den Analysenautomaten Gem-Profiler

Contact Info

Product

Resources

About