Use of a combined slit‐lamp SD‐OCT to obtain anterior and posterior segment images in selected animal species

Rosolen, Serge G.; Rivière, Mei-Lyn K; Lavillegrand, Sylvie; Gautier, B; Picaud, Serge; Legargasson, J. F.

doi:10.1111/j.1463-5224.2012.01037.x

Cited by 26 publications

(33 citation statements)

References 32 publications

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“…With both techniques, there was good definition of retinal layers to allow for qualitative assessment. However, as described by others (McLellan and Rasmussen 2012;Rosolen et al 2012), scans must be carefully examined for errors before tabulating quantitative data. As expected for conventional SDOCT and SSOCT imaging, visualization of the choroidal microvasculature in the minipig eye was obscured by speckle noise (Cheng et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, there is increasing knowledge about the correlation of in vivo OCT scan results with histopathology of the porcine eye (Fatehee et al 2011;Gloesmann et al 2003;Huang et al 2000;Rosolen et al 2012). However, to the best of our knowledge, speckle noise-free OCT techniques including SSOCT and AngioOCT have not yet been described for pigs.…”

Section: Introductionmentioning

confidence: 99%

“…Due to the close anatomical and functional similarity of porcine and human eyes, advanced human ophthalmologic diagnostics can be applied (McLellan and Rasmussen 2012;Rosolen et al 2012). There is increased interest to include optical coherence tomography (OCT) in ocular toxicology studies for safety profiling as it may be used as in vivo biomarker for longitudinal assessment and complement histopathology (McLellan and Rasmussen 2012;Rosolen et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…There is increased interest to include optical coherence tomography (OCT) in ocular toxicology studies for safety profiling as it may be used as in vivo biomarker for longitudinal assessment and complement histopathology (McLellan and Rasmussen 2012;Rosolen et al 2012). This noninvasive technique obtains live subsurface images of ocular structures at near microscopic resolution, which are highly translatable to clinics.…”

Section: Introductionmentioning

confidence: 99%

“…In pigs, ocular fluorescent dye angiography (Galdos et al 2012) and high-quality SDOCT have been established (McLellan and Rasmussen 2012;Rosolen et al 2012). In addition, there is increasing knowledge about the correlation of in vivo OCT scan results with histopathology of the porcine eye (Fatehee et al 2011;Gloesmann et al 2003;Huang et al 2000;Rosolen et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Advanced Clinical Imaging and Tissue-based Biomarkers of the Eye for Toxicology Studies in Minipigs

et al. 2015

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There is increased interest to use minipigs in ocular toxicology studies due to their anatomical similarities with human eyes and as a substitute for nonhuman primates. This requires adaptation of enhanced optical coherence tomography (OCT) techniques and of ocular relevant immunohistochemistry (IHC) or in situ hybridization (ISH) markers to porcine eyes. In this study, OCT and OCT angiography (AngioOCT) were performed on adult Gö ttingen minipigs. To increase structural information on retinal and choroidal vasculature, OCT data were speckle denoized and choroidal blood vessels were segmented with threshold filtering. In addition, we established a set of IHC and ISH markers on Davidson's fixed paraffin-embedded minipig eyes: neurofilament-160, neuronal nuclei, calretinin, protein kinase C-a, vimentin, glial fibrillary acidic protein, glutamine synthetase, ionized calcium-binding adaptor molecule-1, rhodopsin, synaptophysin, postsynaptic density protein-95, retinal pigment epithelium (RPE)-specific protein-65, von Willebrand factor, a-smooth muscle actin, desmin, and Ki-67, thus enabling visualization of retinal neuronal and glial cells, photoreceptors, synapses, RPE, blood vessels, myocytes, macrophages, or cell proliferation. Using ISH, transcripts of vascular endothelial growth factor A, angiopoietin-2, and endothelial tyrosine kinase were visualized. This article describes for the first time in minipig eyes speckle noise-free OCT, AngioOCT, and a set of IHC/ISH markers on Davidson's fixed paraffin-embedded tissues and helps to establish the minipig for ocular toxicology and pharmacology studies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Advanced Clinical Imaging and Tissue-based Biomarkers of the Eye for Toxicology Studies in Minipigs

et al. 2015

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Selection of Appropriate Animal Models in Ocular Research: Ocular Anatomy and Physiology of Common Animal Models

Gilger

Abarca

Salmon

2013

Methods in Pharmacology and Toxicology

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Evaluation of the Taurine Concentrations in Dog Plasma and Aqueous Humour: A Pilot Study

Rosolen

Neveux

Sahel

et al. 2013

Advances in Experimental Medicine and Biology

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In the 70s, the amino acid taurine was found essential for photoreceptor survival. Recently, we found that taurine depletion can also trigger retinal ganglion cell degeneration both in vitro and in vivo. Therefore, evaluation of taurine levels could be a crucial biomarker for different pathologies of retinal ganglion cells such as glaucoma. Because different breeds of dog can develop glaucoma, we performed taurine measurements on plasma and aqueous humour samples from pet dogs. Here, we exposed results from a pilot study on normal selected breed of pet dogs, without any ocular pathology. Samples were collected by veterinarians who belong to the Réseau Européen d'Ophtalmologie Vétérinaire et de Vision Animale. Following measurements by high-performance liquid chromatography (HPLC), the averaged taurine concentration was 162.3 μM in the plasma and 51.8 μM in the aqueous humour. No correlation was observed between these two taurine concentrations, which exhibited a ratio close to 3. Further studies will determine if these taurine concentrations are changed in glaucomatous dogs.

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Use of a combined slit‐lamp SD‐OCT to obtain anterior and posterior segment images in selected animal species

Cited by 26 publications

References 32 publications

Advanced Clinical Imaging and Tissue-based Biomarkers of the Eye for Toxicology Studies in Minipigs

Advanced Clinical Imaging and Tissue-based Biomarkers of the Eye for Toxicology Studies in Minipigs

Selection of Appropriate Animal Models in Ocular Research: Ocular Anatomy and Physiology of Common Animal Models

Evaluation of the Taurine Concentrations in Dog Plasma and Aqueous Humour: A Pilot Study

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