Use of a continuous culture fermentation system to investigate the effect of GanedenBC30 (Bacillus coagulans GBI-30, 6086) supplementation on pathogen survival in the human gut microbiota

Honda, Harue; Gibson, Glenn R.; Farmer, Sean; Keller, David; McCartney, Anne L.

doi:10.1016/j.anaerobe.2010.12.006

Cited by 39 publications

(20 citation statements)

References 34 publications

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“…This inhibition was specific to C. difficile and was not active against other enteropathogens, such as Escherichia coli and Salmonella enterica subsp. enterica serovar Typhimurium 141 . A single Bacillus thuringiensis isolate from the human faecal microbiota has been identified that produces the bacteriocin thuricin CD.…”

Section: Box 1 Origins Of the Concept Of Colonization Resistancementioning

confidence: 99%

Microbiota-mediated colonization resistance against intestinal pathogens

2013

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Commensal bacteria inhabit mucosal and epidermal surfaces in mice and humans, and have effects on metabolic and immune pathways in their hosts. Recent studies indicate that the commensal microbiota can be manipulated to prevent and even to cure infections that are caused by pathogenic bacteria, particularly pathogens that are broadly resistant to antibiotics, such as vancomycin-resistant Enterococcus faecium, Gram-negative Enterobacteriaceae and Clostridium difficile. In this Review, we discuss how immune- mediated colonization resistance against antibiotic-resistant intestinal pathogens is influenced by the composition of the commensal microbiota. We also review recent advances characterizing the ability of different commensal bacterial families, genera and species to restore colonization resistance to intestinal pathogens in antibiotic-treated hosts.

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Section: Box 1 Origins Of the Concept Of Colonization Resistancementioning

confidence: 99%

Microbiota-mediated colonization resistance against intestinal pathogens

2013

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“…B. coagulans could improve growth performance and increase feed digestibility via producing amino acids and vitamins and secreting a-amylase, xylanase, protease and lipidase [34]. Furthermore, consumption of B. coagulans creates a healthy and efficient intestinal environment [43]. For example, when birds were fed a diet with B. coagulans, even though they were infected by Salmonella enteritidis later, their average body weight gain and the feed conversion ratio during the initial stage could still be improved [6].…”

Section: B Coagulans Promotes Nutrient Metabolismmentioning

confidence: 99%

Application of Bacillus coagulans in Animal Husbandry and Its Underlying Mechanisms

Zhou

Zeng

et al. 2020

Animals

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In recent decades, probiotics have attracted widespread attention and their application in healthcare and animal husbandry has been promising. Among many probiotics, Bacillus coagulans (B. coagulans) has become a key player in the field of probiotics in recent years. It has been demonstrated to be involved in regulating the balance of the intestinal microbiota, promoting metabolism and utilization of nutrients, improving immunity, and more importantly, it also has good industrial properties such as high temperature resistance, acid resistance, bile resistance, and the like. This review highlights the effects of B. coagulans in animal husbandry and its underlying mechanisms.

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“…The gastrointestinal microbiome plays an important role in health and disease (reviewed in [ 1 – 8 ]). Although many insights about the role of the microbiota have been gained by studying microbial community association with the human host (e.g., [ 9 – 11 ]), the availability of less complex models of the microbiota (i.e., conventional and humanized animal ([ 12 – 16 ] and in vitro models [ 17 – 22 ]) have also played an important role in elucidating the roles of the microbiota.…”

Section: Introductionmentioning

confidence: 99%

Cultivation of stable, reproducible microbial communities from different fecal donors using minibioreactor arrays (MBRAs)

2015

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BackgroundContinuous-flow culture models are one tool for studying complex interactions between members of human fecal microbiotas because they allow studies to be completed during an extended period of time under conditions where pH, nutrient availability, and washout of waste products and dead cells can be controlled. Because many of the existing well-validated continuous-flow models are large and complex, we were interested in developing a simpler continuous-flow system that would allow microbial community dynamics to be examined in higher throughput while still maintaining complex microbial communities. To this end, we developed minibioreactor arrays (MBRAs), small volume bioreactors (15 ml) that allow simultaneous cultivation of up to 48 microbial communities in a single anaerobic chamber.ResultsWe used MBRA to characterize the microbial community dynamics of replicate reactors inoculated from three different human fecal donors and reactors seeded with feces pooled from these three donors. We found that MBRA could be used to efficiently cultivate complex microbial communities that were a subset of the initial fecal inoculum (15–25 % of fecal OTUs initially observed). After an initial acclimation period of approximately 1 week, communities in each reactor stabilized and exhibited day-to-day variation similar to that observed in stable mouse fecal communities. Replicate reactors were predominately populated by shared core microbial communities; variation between replicate reactors was primarily driven by shifts in abundance of shared operational taxonomic units (OTUs). Consistent with differences between fecal donors, MBRA communities present in reactors seeded with different fecal samples had distinct composition and structure.ConclusionsFrom these analyses, we conclude that MBRAs can be used to cultivate communities that recapitulate key features of human fecal communities and are a useful tool to facilitate higher-throughput studies of the dynamics of these communities.Electronic supplementary materialThe online version of this article (doi:10.1186/s40168-015-0106-5) contains supplementary material, which is available to authorized users.

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Use of a continuous culture fermentation system to investigate the effect of GanedenBC30 (Bacillus coagulans GBI-30, 6086) supplementation on pathogen survival in the human gut microbiota

Cited by 39 publications

References 34 publications

Microbiota-mediated colonization resistance against intestinal pathogens

Microbiota-mediated colonization resistance against intestinal pathogens

Application of Bacillus coagulans in Animal Husbandry and Its Underlying Mechanisms

Cultivation of stable, reproducible microbial communities from different fecal donors using minibioreactor arrays (MBRAs)

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