Use of a Four-Cell Conductivity Bridge as a Continuous Detector for Ion Exchange Chromatography.

Abstract

A device capable of continuously detecting aqueous ionic components emerging from a chromatographic column is based on the measurement

Oscillometric flow cell for measurement of conductivity and permittivity

“…Conductometric and permittivity detectors have already been applied to liquid chromatography. With these cells small cell volume (1-5), high sensitivity (6,7), and wide range of linearity are requirements. In respect of the development of conductivity detectors for ion chromatography the papers presented by Evans et al (8) and Jupille et al It appears that in all conductometric and permittivity detectors developed so far there is a direct galvanic contact between the electrodes and the solution tested.…”

“…A suitable microcomputer interface for implementing such an instrumentation system has been recently described by Ben-Yaakov et al (6). Frevert and Galster (7) proposed an analog method for direct determination of total sulfide by using a sulfide ion selective electrode coupled to a pH glass electrode. This method is limited to solutions with pHs <6.0.…”

“…THEORETICAL CONSIDERATIONS Total concentration of sulfide (ST) in solution can be expressed as (7) and brackets denote concentrations. The K's are apparent constants (7) which are defined in terms of both activity (oH+) and concentrations.…”

Biochemical Analysis

“…The antigens, being rendered insoluble, removed certain antibodies; enzymatic activity of the enmeshed enzymes could also be demonstrated (19). Technological innovations consisted of a linear gradient mixing device for viscous solutions (64), a programming system for the automatic amino acid analyzer (321), a sample injector and tubular type flow cell (79), and the use of a conductivity bridge as a continuous detector for ionic components emerging in an aqueous column effluent (94).…”