Use of a hyperdried cross-linked amniotic membrane as initial therapy for corneal perforations

Kitagawa, Kiyotaka; Okabe, Motonori; Yanagisawa, Shuichiro; Zhang, Xueyun; Nikaido, Toshio; Hayashi, Atsushi

doi:10.1007/s10384-010-0903-0

Cited by 24 publications

(25 citation statements)

References 21 publications

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“…In addition dried AM preparations, cross-linked with glutaraldehyde, have been used as a primary treatment for corneal perforations, indicating its increased usefulness over cryopreserved AM in certain clinical situations[51]. This may reflect easier surgical handling of the dried tissue, which can be glued rather than sutured onto the ocular surface[30].…”

Section: Discussionmentioning

confidence: 99%

Augmented Dried versus Cryopreserved Amniotic Membrane as an Ocular Surface Dressing

et al. 2013

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PurposeDried amniotic membrane (AM) can be a useful therapeutic adjunct in ophthalmic surgery and possesses logistical advantages over cryopreserved AM. Differences in preservation techniques can significantly influence the biochemical composition and physical properties of AM, potentially affecting clinical efficacy. This study was established to investigate the biochemical and structural effects of drying AM in the absence and presence of saccharide lyoprotectants and its biocompatibility compared to cryopreserved material.MethodsAM was cryopreserved or dried with and without pre-treatment with trehalose or raffinose and the antioxidant epigallocatechin (EGCG). Structural and visual comparisons were assessed using electron microscopy. Localisation, expression and release of AM biological factors were determined using immunoassays and immunofluorescence. The biocompatibility of the AM preparations co-cultured with corneal epithelial cell (CEC) or keratocyte monolayers were assessed using cell proliferation, cytotoxicity, apoptosis and migration assays.ResultsDrying devitalised AM epithelium, but less than cryopreservation and cellular damage was reduced in dried AM pre-treated with trehalose or raffinose. Dried AM alone, and with trehalose or raffinose showed greater factor retention efficiencies and bioavailability compared to cryopreserved AM and demonstrated a more sustained biochemical factor time release in vitro. Cellular health assays showed that dried AM with trehalose or raffinose are compatible and superior substrates compared to cryopreserved AM for primary CEC expansion, with increased proliferation and reduced LDH and caspase-3 levels. This concept was supported by improved wound healing in an immortalised human CEC line (hiCEC) co-cultured with dried and trehalose or raffinose membranes, compared to cryopreserved and fresh AM.ConclusionsOur modified preservation process and our resultant optimised dried AM has enhanced structural properties and biochemical stability and is a superior substrate to conventional cryopreserved AM. In addition this product is stable and easily transportable allowing it to be globally wide reaching for use in clinical and military sectors.

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Section: Discussionmentioning

confidence: 99%

Augmented Dried versus Cryopreserved Amniotic Membrane as an Ocular Surface Dressing

et al. 2013

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“…AM was used in clinic for many decades for wound healing in burns, promoting epithelium formation and protecting against infection [39, 40]. Recently, the use of AM has been evaluated as a wound dressing material for surgical defects of the oral mucosa [41], ocular surface reconstruction [40, 42], corneal perforations [43, 44], and bladder augmentation [45]. …”

Section: Introductionmentioning

confidence: 99%

Amniotic Fluid and Amniotic Membrane Stem Cells: Marker Discovery

Roubelakis

Trohatou

Anagnou

2012

Stem Cells International

103

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Amniotic fluid (AF) and amniotic membrane (AM) have been recently characterized as promising sources of stem or progenitor cells. Both not only contain subpopulations with stem cell characteristics resembling to adult stem cells, such as mesenchymal stem cells, but also exhibit some embryonic stem cell properties like (i) expression of pluripotency markers, (ii) high expansion in vitro, or (iii) multilineage differentiation capacity. Recent efforts have been focused on the isolation and the detailed characterization of these stem cell types. However, variations in their phenotype, their heterogeneity described by different groups, and the absence of a single marker expressed only in these cells may prevent the isolation of a pure homogeneous stem cell population from these sources and their potential use of these cells in therapeutic applications. In this paper, we aim to summarize the recent progress in marker discovery for stem cells derived from fetal sources such as AF and AM, using novel methodologies based on transcriptomics, proteomics, or secretome analyses.

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“…The hyperdry device and sterilization by γ‐irradiation at 25 kGy, which has been recommended for terminal sterilization of medical products, can completely prevent known transmissible infections, and no adverse effects have been reported in patients who received HD‐AM grafts . Bacteriologic test also indicated that 25 kGy γ‐irradiation achieved to complete sterilization(data not shown).…”

Section: Discussionmentioning

confidence: 94%

“…HD‐AM was also shown to reduce wound dehydration. The details of these benefits are described in clinical observations and case reports …”

Section: Introductionmentioning

confidence: 99%

“…The details of these benefits are described in clinical observations and case reports. [29][30][31][32][33][34][35][36][37] The purposes of the present study were to (1) compare the physical and histological properties of HD-AM with those of freeze-dried human amniotic membrane (FD-AM) processed with typical freeze-dry methods, (2) evaluate the safety of HD-AM, and (3) demonstrate the feasibility of HD-AM transplantation in a case of recurrent pterygium.…”

Section: Introductionmentioning

confidence: 99%

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Hyperdry human amniotic membrane is useful material for tissue engineering: Physical, morphological properties, and safety as the new biological material

Okabe

Kitagawa

Yoshida

et al. 2013

J Biomedical Materials Res

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Human amniotic membrane (AM) has been used widely as graft biomaterial for a variety of clinical applications. But, there are some persistent problems related to the preparation, storage, and sterilization. To resolve these problems, we developed hyperdry AM (HD-AM) using far-infrared rays, depression of air, and microwaves and then sterilized by γ-ray irradiation. To elucidate the benefit of HD-AM as biological materials, compare with the physical and histological properties of HD-AM with a freeze-dried AM (FD-AM) as typical freeze-dried methods, evaluate the safety of HD-AM in vivo experiment used nude mice, and demonstrate the feasibility of HD-AM transplant in pterygium. The water permeability and the sieving coefficient of HD-AM were significantly lower than that of FD-AM. HD-AM has kept the morphological structure of epithelium and connective tissues. At 18 months after transplanted, single and multilayers of HD-AM in the intraperitoneal cavity was degraded without any infiltrated cells. For clinical treatment, recurrence of pterygium and regrowth of the subconjunctival fibrosis were not observed during the 6-month follow-up periods after the surgery. It was proposed that HD-AM was a safe and effective new biological material for clinical use including treatment for recurrent pterygium.

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Use of a hyperdried cross-linked amniotic membrane as initial therapy for corneal perforations

Cited by 24 publications

References 21 publications

Augmented Dried versus Cryopreserved Amniotic Membrane as an Ocular Surface Dressing

Augmented Dried versus Cryopreserved Amniotic Membrane as an Ocular Surface Dressing

Amniotic Fluid and Amniotic Membrane Stem Cells: Marker Discovery

Hyperdry human amniotic membrane is useful material for tissue engineering: Physical, morphological properties, and safety as the new biological material

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