Use of a Keratin-Based Wound Dressing in the Management of Wounds in a Patient with Recessive Dystrophic Epidermolysis Bullosa

Kirsner, Robert S.; Cassidy, Sharon; Marsh, Clive; Vivas, Alejandra C.; Kelly, Robert J.

doi:10.1097/01.asw.0000419404.44947.de

Cited by 28 publications

(13 citation statements)

References 4 publications

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“…Keratins can self-assemble and be crosslinked to produce porous or fibrous scaffolds [ 32 , 34 , 35 , 36 , 37 ]. In particular, keratin is widely used as a wound dressing or scaffold for skin regeneration [ 38 ], as keratin promotes cell adhesion or the proliferation of keratinocytes or fibroblasts [ 20 , 21 ]. Although keratin has poor mechanical properties, various studies have been conducted to improve mechanical strength, while having good cell affinity, through mixing with various biomaterials [ 39 , 40 , 41 , 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Development of a Multi-Layer Skin Substitute Using Human Hair Keratinic Extract-Based Hybrid 3D Printing

et al. 2021

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Large-sized or deep skin wounds require skin substitutes for proper healing without scar formation. Therefore, multi-layered skin substitutes that mimic the genuine skin anatomy of multiple layers have attracted attention as suitable skin substitutes. In this study, a novel skin substitute was developed by combining the multi-layer skin tissue reconstruction method with the combination of a human-derived keratinic extract-loaded nano- and micro-fiber using electrospinning and a support structure using 3D printing. A polycaprolactone PCL/keratin electrospun scaffold showed better cell adhesion and proliferation than the keratin-free PCL scaffold, and keratinocytes and fibroblasts showed better survival, adhesion, and proliferation in the PCL/keratin electrospun nanofiber scaffold and microfiber scaffold, respectively. In a co-culture of keratinocytes and fibroblasts using a multi-layered scaffold, the two cells formed the epidermis and dermal layer on the PCL/keratin scaffold without territorial invasion. In the animal study, the PCL/keratin scaffold caused a faster regeneration of new skin without scar formation compared to the PCL scaffold. Our study showed that PCL/keratin scaffolds co-cultured with keratinocytes and fibroblasts promoted the regeneration of the epidermal and dermal layers in deep skin defects. Such finding suggests a new possibility for artificial skin production using multiple cells.

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Section: Discussionmentioning

confidence: 99%

Development of a Multi-Layer Skin Substitute Using Human Hair Keratinic Extract-Based Hybrid 3D Printing

et al. 2021

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“…Kirsner et al. [17] reported that a combination of keratin solution and a gauze bandage shows a highly improved healing effect on wound sites. Paladini et al.…”

Section: Introductionmentioning

confidence: 99%

Characterization and study of physical properties and antibacterial activities of human hair keratin–silver nanoparticles and keratin–gold nanoparticles coated cotton gauze fabric

Shanmugasundaram¹,

Ramkumar²

2016

Journal of Industrial Textiles

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Human hairs are considered as major waste from barbershops. It contains an important protein called keratin. Keratin from human hair exhibits biocompatibility, wound-healing property, biodegradability, and antibacterial property. Silver and gold nanoparticles are commonly used in wound-healing applications. These nanoparticles possess excellent functional properties like antibacterial, wound healing and biocompatibility and applicable in biomedical field. In this study, silver and gold nanoparticles were used along with human hair keratin for the production of antibacterial materials. The functional properties of these two materials are synergized for wound-healing applications. Silver and gold nanoparticles were synthesized by chemical reduction method. The synthesized nanoparticles were characterized using UV–Visible, particle size, and zeta potential analyses. The prepared materials were characterized by scanning electron microscopy, Fourier transform infrared, and energy-dispersive X-ray spectroscopic analyses and they were measured for the physical properties such as air permeability, water contact angle, moisture content, and water absorbency. Furthermore, the antibacterial activities were evaluated against burn wound bacteria. Superior antibacterial activities and adequate physical properties found in keratin/nanoparticles immobilized cotton samples.

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“…[5] After treatment, it was observed that the number of blisters and the epithelialization period of the eroded areas significantly decreased in areas subjected to keratin application. [5] After treatment, it was observed that the number of blisters and the epithelialization period of the eroded areas significantly decreased in areas subjected to keratin application.…”

mentioning

confidence: 99%

Lack of effectiveness of keratin dressings in epidermolysis bullosa

Sürücü¹,

Yeşilova²,

Turan³

et al. 2015

Indian J Dermatol Venereol Leprol

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Use of a Keratin-Based Wound Dressing in the Management of Wounds in a Patient with Recessive Dystrophic Epidermolysis Bullosa

Cited by 28 publications

References 4 publications

Development of a Multi-Layer Skin Substitute Using Human Hair Keratinic Extract-Based Hybrid 3D Printing

Development of a Multi-Layer Skin Substitute Using Human Hair Keratinic Extract-Based Hybrid 3D Printing

Characterization and study of physical properties and antibacterial activities of human hair keratin–silver nanoparticles and keratin–gold nanoparticles coated cotton gauze fabric

Lack of effectiveness of keratin dressings in epidermolysis bullosa

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