Use of a new assay technique for quantification of antifungal activity of nystatin incorporated in denture liners

Truhlar, Mary R.; Shay, Kenneth; Sohnle, Peter G.

doi:10.1016/0022-3913(94)90193-7

Cited by 74 publications

(134 citation statements)

References 11 publications

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“…6 Several in vitro and in vivo studies demonstrated that the integration of fungicidal factors into tissue conditioners are useful and practical for the inhibition or prevention of plaque formation and improved recovery in denture stomatitis. 6,21,[25][26][27][28][29] Gruber et al 30 reported that silicone tissue conditioners and soft liners impregnated with zinc undecylenate eliminated colonization by Candida. In microbiological assays, nystatin restricted the emergence of C. albicans when it was used with a tissue conditioner.…”

Section: Although the Etiology Of Denture Stomatitis Is Multifactoriamentioning

confidence: 99%

“…2 According to the literature, this condition occurs in between 11% and 67% of denture wearers. [3][4][5] Local factors associated with dentures are also connected with denture stomatitis, such as the presence of a biofilm, [6][7][8][9] local trauma caused by dentures, 10 xerostomia, 11 continuous use of dentures, 12 and alteration in the salivary pH. 12 Soft denture liners, namely soft polymers, can be applied to the mucosal or fitting area of the dentures.…”

Section: Introductionmentioning

confidence: 99%

“…28 In a study of the antimycotic action of nystatin-impregnated soft denture lining materials and the influence of 8 an aqueous environment on the fungicidal characteristics, Truhlar et al 6 showed that doses of 1,000,000 U and 500,000 U were useful in maintaining the drug-leaching quantity above the minimum inhibitory concentration for antifungal action. Radnai et al 12 demonstrated that miconazole gel, combined with a Viscogel tissue conditioner, restricted the emergence of C.…”

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confidence: 99%

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Comparison of the Effect of Nystatin and Titanium Dioxide Addition on Candida Colonization in Soft Lining Material

Bayındır¹,

Aktaş²,

Baştopçu³

2017

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

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Material and methods. Disks of Ufi Gel P and Mollosil cold-curing, soft relining materials with a diameter of 5 mm and thickness of 2 mm were prepared with nystatin and TiO 2 powders in a concentration of 5%, 10%, and 15% (w/v). For the control group, only discs not containing nystatin and TiO 2 were prepared, which consisted only of soft lining material. C. albicans was inoculated on Sabouraud dextrose agar (SDA)-coated plates. The disks were placed on these plates and incubated aerobically at 37° C.The Ufi Gel P and Mollosil disks, including 10% w/v nystatin, (re-prepared for the second step of the study) were immersed in water for separate intervals (1, 7, 14, and 16 days) to determine the fungicidal action over time. The disks were then removed and placed on SDA plates inoculated with C. albicans. In each group, one control disk without nystatin or TiO 2 was included. Results.A univariate analysis of variance test revealed that the activity of C. albicans was not significantly inhibited on the control disks (p < 0.001). The addition of nystatin in powder form to the soft liners prevented the colonization of C. albicans in vitro. The immersion time of the disks plunged in water appeared to affect the degree of inhibition. However, the addition of TiO 2 did not prevent colonization by C. albicans of the soft liners.Conclusions. This study showed that the addition of nystatin in powder form to soft liners prevented the colonization of C. albicans in vitro but that the addition of the same amount of TiO 2 powder did not.

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Section: Although the Etiology Of Denture Stomatitis Is Multifactoriamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Comparison of the Effect of Nystatin and Titanium Dioxide Addition on Candida Colonization in Soft Lining Material

Bayındır¹,

Aktaş²,

Baştopçu³

2017

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

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“…A degradação e enrijecimento gradual apresentada pelos materiais resilientes temporários implica em sua substituição por similares ou por reembasadores de longo prazo, não devendo ultrapassar 14 dias, período esse semelhante ao de um tratamento de estomatite protética com antifúngico tópico convencional (SCHNEID, 1992;TRUHLAR;SHAY;SOHNLE, 1994). Nos casos de perda de adaptação das bases das próteses com a área de suporte, o uso de reembasadores resilientes contendo antimicrobianos minimiza o trauma tecidual e previne o acúmulo de biofilme até que o problema seja solucionado por novas próteses ou reembasamento laboratorial.…”

Section: Introductionunclassified

“…A modificação de condicionadores de tecido pela incorporação de agentes antimicrobianos em concentrações padronizadas tem demonstrado ser efetiva e viável na inibição de C. albicans (CARTER; KERR; SHEPHERD, 1986;FALAH-TAFTI et al, 2010;QUINN, 1985;RADNAI et al, 2010;SCHNEID, 1992;NUTT, 1978;TRUHLAR;SHAY;SOHNLE, 1994). Entretanto, estudos prévios demonstraram que a adição de fármacos em concentrações comercialmente disponíveis a materiais poliméricos/plásticos, incluindo algumas marcas comerciais de condicionadores de tecido e reembasadores resilientes, pode afetar suas caraterísticas estruturais (ADDY; HANDLEY, 1981), (URBAN et al, 2009), módulo…”

Section: Introductionunclassified

Viabilidade do uso de dispositivo acrílico intra-oral reembasado por material resiliente temporário modificado por antimicrobianos: estudo preliminar em ratos

Hotta¹

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Antifungal activity of DNA–lipid complexes and DNA–lipid films against Candida species

Inoue

Fukushima

Hayakawa

et al. 2005

J Biomedical Materials Res

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In this study amphiphilic lipids, DNA-lipid complexes, and DNA-lipid films were prepared, and their antifungal activity against Candida species was examined. The amphiphilic lipids were synthesized from a reaction of glycine or L-alanine with n-alkyl alcohol in the presence of p-toluene sulfonic acid. DNA-lipid complexes, which were prepared by the simple mixing of DNA and amphiphilic lipids, were insoluble in water. Self-standing, water-insoluble DNA-lipid films were prepared by casting the DNA-lipid complexes from a chloroform/ethanol solution. The antifungal activities of the lipids and DNA-lipid complexes against the Candida species were evaluated by minimum inhibitory concentrations (MICs); those of DNA-lipid films were evaluated by the disk diffusion method. The seven kinds of lipids, DNA-lipid complexes, and DNA-lipid films showed antifungal activity, and no differences were seen in the antifungal activities between glycine and L-alanine derivatives. The lipids, DNA-lipid complexes, and DNA-lipid films, which have shorter alkyl chain length in lipids, showed antifungal activity against all Candida species. However, the effect of antifungal activity against Candida species decreased with increased alkyl chain length in lipids. In this study, it was found that lipids, DNA-lipid complexes, and films with a decyl or dodecyl group exhibit more favorable antifungal activity.

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Use of a new assay technique for quantification of antifungal activity of nystatin incorporated in denture liners

Cited by 74 publications

References 11 publications

Comparison of the Effect of Nystatin and Titanium Dioxide Addition on Candida Colonization in Soft Lining Material

Comparison of the Effect of Nystatin and Titanium Dioxide Addition on Candida Colonization in Soft Lining Material

Viabilidade do uso de dispositivo acrílico intra-oral reembasado por material resiliente temporário modificado por antimicrobianos: estudo preliminar em ratos

Antifungal activity of DNA–lipid complexes and DNA–lipid films against Candida species

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