Use of a Potassium Tellurite Medium in the Detection of Corynebacterium diphtheriae

Kellogg, Doris K.; Wende, Reuben D.

doi:10.2105/ajph.36.7.739

Am J Public Health Nations Health

1946

DOI: 10.2105/ajph.36.7.739

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Use of a Potassium Tellurite Medium in the Detection of Corynebacterium diphtheriae

Doris K. Kellogg¹,

Reuben D. Wende²

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1949

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Cited by 3 publications

(1 citation statement)

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“…A disadvantage of incorporating tellurium or tetrazolium salts into the medium is that they inhibit bacterial growth in varying degree (9, 10,11) . Therefore, we decided to eliminate the reacting agent from the medium.…”

mentioning

confidence: 99%

Tetrazolium Salts and Identification of Corynebacterium diphtheriae.

Monis

Reback

1962

Experimental Biology and Medicine

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mentioning

confidence: 99%

Tetrazolium Salts and Identification of Corynebacterium diphtheriae.

Monis

Reback

1962

Experimental Biology and Medicine

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A plasmid locus associated with Klebsiella clinical infections encodes a microbiome-dependent gut fitness factor

et al. 2021

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Klebsiella pneumoniae (Kp) is an important cause of healthcare-associated infections, which increases patient morbidity, mortality, and hospitalization costs. Gut colonization by Kp is consistently associated with subsequent Kp disease, and patients are predominantly infected with their colonizing strain. Our previous comparative genomics study, between disease-causing and asymptomatically colonizing Kp isolates, identified a plasmid-encoded tellurite (TeO3-2)-resistance (ter) operon as strongly associated with infection. However, TeO3-2 is extremely rare and toxic to humans. Thus, we used a multidisciplinary approach to determine the biological link between ter and Kp infection. First, we used a genomic and bioinformatic approach to extensively characterize Kp plasmids encoding the ter locus. These plasmids displayed substantial variation in plasmid incompatibility type and gene content. Moreover, the ter operon was genetically independent of other plasmid-encoded virulence and antibiotic resistance loci, both in our original patient cohort and in a large set (n = 88) of publicly available ter operon-encoding Kp plasmids, indicating that the ter operon is likely playing a direct, but yet undescribed role in Kp disease. Next, we employed multiple mouse models of infection and colonization to show that 1) the ter operon is dispensable during bacteremia, 2) the ter operon enhances fitness in the gut, 3) this phenotype is dependent on the colony of origin of mice, and 4) antibiotic disruption of the gut microbiota eliminates the requirement for ter. Furthermore, using 16S rRNA gene sequencing, we show that the ter operon enhances Kp fitness in the gut in the presence of specific indigenous microbiota, including those predicted to produce short chain fatty acids. Finally, administration of exogenous short-chain fatty acids in our mouse model of colonization was sufficient to reduce fitness of a ter mutant. These findings indicate that the ter operon, strongly associated with human infection, encodes factors that resist stress induced by the indigenous gut microbiota during colonization. This work represents a substantial advancement in our molecular understanding of Kp pathogenesis and gut colonization, directly relevant to Kp disease in healthcare settings.

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A Transparent Dextrose Serum Tellurite Plating Medium: Its Use as an Adjunct to Microscopic Examination of Smears Made from Loeffler Slants in Routine Diphtheria Diagnosis

Or¹,

Damon²

1949

Public Health Reports (1896-1970)

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The puirpose of this study was to develop a plating medium on which C. diphtheriae would exhibit differential colonial features. With such a medium, recognition of these characteristics could be used in the diagnostic laboratory to supplement the study of cellular morphology in smears made from Loeffler slants. Diagnostic reporting should thus become more objective and more reliable. The usefulness of such a medium would depend on whether or not diphtheria colonies would be large enough to show their characteristics after 18-24 hours incubation, that is, at the time diagnostic smears are usually made. Furthermore this plating medium should be inhibitory for as many as possible of the commensal organisms found in nose and throat cultures.

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Use of a Potassium Tellurite Medium in the Detection of Corynebacterium diphtheriae

Cited by 3 publications

References 19 publications

Tetrazolium Salts and Identification of Corynebacterium diphtheriae.

Tetrazolium Salts and Identification of Corynebacterium diphtheriae.

A plasmid locus associated with Klebsiella clinical infections encodes a microbiome-dependent gut fitness factor

A Transparent Dextrose Serum Tellurite Plating Medium: Its Use as an Adjunct to Microscopic Examination of Smears Made from Loeffler Slants in Routine Diphtheria Diagnosis

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