Use of a rep-PCR system to predict species in the Aspergillus section Nigri

Palencia, Edwin R.; Klich, M. A.; Glenn, Anthony E.; Bacon, Charles W.

doi:10.1016/j.mimet.2009.07.012

Cited by 28 publications

(26 citation statements)

References 37 publications

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“…Giraud et al [25] reported that A. awamori was not a separate species within the A. niger aggregate. Besides, the species A. foetidus , based on β -tubulin analysis, is considered synonyms to A. niger [26, 27]. …”

Section: Resultsmentioning

confidence: 99%

Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger

et al. 2011

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A fungal isolate with capability to grow in keratinous substrate as only source of carbon and nitrogen was identified as Aspergillus niger using the sequencing of the ITS region of the rDNA. This strain produced a slightly acid keratinase and an acid protease during cultivation in feather meal. The peak of keratinolytic activity occurred in 48 h and the maximum proteolytic activity in 96 h. These enzymes were partly characterized as serine protease and aspartic protease, respectively. The effects of feather meal concentration and initial pH on enzyme production were evaluated using a central composite design combined with response surface methodology. The optimal conditions were determined as pH 5.0 for protease and 7.8 for keratinase and 20 g/L of feather meal, showing that both models were predictive. Production of keratinases by A. niger is a less-exploited field that might represent a novel and promising biotechnological application for this microorganism.

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Section: Resultsmentioning

confidence: 99%

Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger

et al. 2011

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“…REP-PCR and ERIC-PCR techniques have been used in molecular fi ngerprinting of various other bacterial strains as well as fungus (Genersch and Otten, 2003;Palencia et al, 2009;Szczuka and Kaznowski, 2004). A collection of 120 Aeromonas sp.…”

Section: Eric-pcr Fi Ngerprinting Of B Thuringiensismentioning

confidence: 99%

“…In classifi cation of Rhizobium strains, REP-PCR and ERIC-PCR were as useful as multilocus enzyme electrophoresis (de Bruijn, 1992). In a recent study, the REP-PCR technique has also been found effective for the fi ngerprinting of fungal species Aspergillus niger (Palencia et al, 2009).…”

Section: Eric-pcr Fi Ngerprinting Of B Thuringiensismentioning

confidence: 99%

Molecular typing of native <i>Bacillus thuringiensis</i> isolates from diverse habitats in India using REP-PCR and ERIC-PCR analysis

Katara¹,

Deshmukh²,

Singh³

et al. 2012

J. Gen. Appl. Microbiol.

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IntroductionBacillus thuringiensis is a rod-shaped, gram-positive, endospore-forming bacterium. It is distinguished from three other closely related Bacillus spp., viz. B. cereus, B. anthracis and B. mycoides, because of its ability to synthesize delta endotoxins (Cry proteins) as protein inclusion crystals during sporulation (Hofte and Whiteley, 1989). The presence of a parasporal crystal, which is outside the exosporium of the endospore, is indicative of production of the toxin, and serves as a marker for this species. The bacterium was initially discovered as a pathogen of various insects and was fi rst used as an insecticidal agent. It is found in soil where it leads a saprophytic existence, but becomes an opportunistic pathogen of insects when ingested. The delta-endotoxin causes midgut paralysis and disruption when ingested by the insect host. The specifi c activities of the toxin towards insects and nontoxic nature toward animals have made this organism a useful biocontrol agent. The insecticidal crystal (Cry) J. Gen. Appl. Microbiol., 58, 83 94 (2012) Bacillus thuringiensis is a bacterium of great agronomic and scientifi c interest. The subspecies of this bacterium colonize and kill a large variety of host insects and even nematodes, but each strain does so with a high degree of specifi city. Therefore molecular typing and diversity analysis of B. thuringiensis has enormous importance for discrimination of strains isolated from different sources. In this study, 113 native B. thuringiensis isolates collected from diverse habitats and locations in India and 27 B. thuringiensis type strains obtained from the Bacillus Genetic Stock Centre (BGSC), Ohio State University, USA and used as reference, were analyzed for molecular typing. Genotypic data of 140 B. thuringiensis isolates and type strains was generated by using REP-PCR and ERIC-PCR primers and unweighted pair group method with arithmetic mean (UPGMA) analysis using NTSYSpc2.2 and grouped into 4 main clusters. All the groups have isolates from diverse origins. No group was found to represent any specifi c origin or location. The observed patterns of REP-PCR and ERIC-PCR pattern were discriminatory enough to reveal differences in the B. thuringiensis isolates and reference strains. The resolution power and marker index of the ERIC-PCR (RP 9.39, MI 6.34) was found to be higher than that of the REP-PCR (RP 6.20, MI 4.48). The REP-PCR and ERIC-PCR markers have been found to be useful for discrimination of B. thuringiensis isolates and reference strains. ERIC-PCR was the more informative of the two techniques. This study showed that the B. thuringiensis isolates collected from diverse habitats in India had a high degree of genetic diversity.

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“…Taken together, the results obtained in this study indicate that the RAPD and ISSR primers amplify random regions in the genome of Trichoderma spp., and no relationships were found among the amplified regions [14,25]. Nevertheless, RAPD-PCR and ISSR-PCR are highly reproducible methods for the characterization of fungal species [5,26,27]. The ISSR-PCR approach has shown to be useful for assessing the genetic diversity of Trichoderma isolates and their corresponding fusants are obtained by protoplast fusion [2,6,25], and represents a valid alternative to the RAPD technique permitting fingerprinting of the genomes by using fewer primers.…”

Section: Resultsmentioning

confidence: 99%

Fingerprinting and molecular comparison among two parental strains of Trichoderma spp. and their corresponding fusants produced by protoplast fusion

Lakhani

Vakharia

Hassan

et al. 2016

Biotechnology & Biotechnological Equipment

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Protoplast fusions are important tools in strain improvement for bringing genetic recombination and developing hybrid strains in filamentous fungi. These are the powerful techniques for engineering of microbial strains with desirable different properties. The molecular characterization of two parent's strains (Trichoderma harzianum NBAII Th 1, Trichoderma viride NBAII Tv 23) and their corresponding fusants, produced by protoplast fusion, was investigated by random amplified polymorphic DNA (RAPD), ISSR (inter-simple sequence repeats) and internal transcribed spacer (ITS) markers. Specific results for fingerprinting were obtained by the 20 primers of each RAPD and ISSR, and four primers of ITS, respectively. These markers produced different fragment patterns with varied number of bands and yielded a total of 419 distinct bands. 13.3% were considered as polymorphic bands and 86.7% were considered as monomorphic bands. For RAPD marker, the OPC-04 primer has showed the highest polymorphism, 58.3%. While in ISSR marker, the UBC-835 primer has showed the highest polymorphism, 57.2%. The dendrogram based on RAPD marker results grouped the two parent's strains and 21 fusants into two different clusters with about 97% genetic similarity. While the dendrogram based on ISSR and ITS markers results grouped the two parent's strains and 21 fusants into two different clusters with about 88% genetic similarity. Moreover, the smallest genetic distance (0.01) was estimated between fusant-10 and fusant-12; moreover, fusant-8 and fusant-20 relatively have the highest genetic distance. Our results are consistent with the conclusion that understanding the genetic variation within parent Trichoderma strains and their corresponding fusants and molecular occurrence of protoplast fusion using RAPD, ISSR and ITS markers.

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Use of a rep-PCR system to predict species in the Aspergillus section Nigri

Cited by 28 publications

References 37 publications

Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger

Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger

Molecular typing of native <i>Bacillus thuringiensis</i> isolates from diverse habitats in India using REP-PCR and ERIC-PCR analysis

Fingerprinting and molecular comparison among two parental strains of Trichoderma spp. and their corresponding fusants produced by protoplast fusion

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