ABSTRACT. An acoustic plethysmograph designed to measure body volumes of infants is described. This method uses the principle of the Helmholtz resonator in which the resonant frequency of a chamber is inversely proportional to the square root of the volume of air inside the chamber. After an object is placed inside the chamber, the change in resonant frequency is used to measure the volume of the object. The coefficient of variation of repeated measures of body volumes of 13 miniature piglets (5 to 18 days old, body weights 1253 to 2631 g) ranged from 0.3 to 3.2%. Body volumes measured by the acoustic method agree closely with those calculated from carcass analysis in which body volume is the sum of the volumes of total body water, fat, protein, and minerals. Fat-free mass computed from densitometry using a four-compartment model and body volume measurements from the acoustic method agrees with fat-free mass obtained from carcass analysis. The mean difference between methods was not significant: 1 ml for body volume and 3 g for fat-free mass. The limits of agreement between methods was f 75 ml for body volume and f 214 g for fat-free mass. Our results indicate that the acoustic method can measure a change in body volume of 75 ml and a change in fat-free mass of 214 g in a 2000 g infant. (Pediatr Res 24: 85-89, 1988) (7), pressure differential (8, 9), and a combination of water and air displacement (10, 11) have been explored. None of those methods is without technical difficulties: e.g. changes in chamber pressures due to alterations in chamber temperature and respiratory gas exchange and evaporative water losses from the subjects.In collaboration with colleagues from the University of Houston, we developed an acoustic plethysmograph, based on the Helmholtz resonator principle (12, 13). A detailed discussion of the theory and construction of an early prototype resonating chamber has been published (12, 13). Preliminary validation of this method based on measurements of inanimate objects and anesthetized infant miniature pigs demonstrated the potential usefulness of this method for body volume measurements in infants (12, 13).We designed the present experiment to assess the validity of the acoustic plethysmograph to measure body volumes and to estimate the fat-free mass of anesthetized infant miniature pigs. Values of body volume obtained by the acoustic method were compared with estimates of body volume calculated from the chemical analysis of the whole carcass. Values of fat-free mass estimated from body density were compared with those obtained from carcass analysis.
METHODSAssessments of energy metabolism and requirements of infants have been impeded by the paucity of acceptable methods to determine body composition. Of the various techniques that have been explored, those based on densitometry (1) have the most appeal because they are noninvasive and can be used repeatedly over short periods.The determination of body density requires the measurement of body mass and volume. Although accurate measures of body...