Use of a Smartphone as a Colorimetric Analyzer in Paper-based Devices for Sensitive and Selective Determination of Mercury in Water Samples

Jarujamrus, Purim; Meelapsom, Rattapol; Pencharee, Somkid; Obma, Apinya; Amatatongchai, Maliwan; Ditcharoen, Nadh; Chairam, Sanoe; Tamuang, Suparb

doi:10.2116/analsci.34.75

Cited by 52 publications

(17 citation statements)

References 20 publications

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“…Microfluidic paper-based analytical devices (μPADs) have gained attention and have been successfully used as a detection platform for several applications, including environmental monitoring, health diagnosis and food safety. [23][24][25] The μPADs can be simply made from inexpensive chromatography or filter paper. They are fabricated by forming hydrophobic barriers in hydrophilic paper producing hydrophilic channels for liquid flow.…”

Section: Introductionmentioning

confidence: 99%

Paper-based DPPH Assay for Antioxidant Activity Analysis

2018

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We report on a paper-based 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) assay for a simple, inexpensive, low reagent and sample consumption and high throughput analysis of antioxidant activity. The paper-based device was fabricated using a lamination method to create a 5-mm in diameter circular test zone that was embedded with a DPPH reagent. The analysis was carried out in one-step by dropping an antioxidant/sample onto the test zone. After reduction by the antioxidant, the DPPH radicals become stable DPPH molecules, resulting in a change in color from deep violet to pale yellow. The violet color intensity of DPPH was inversely proportional to the antioxidant activity of the samples, and was measured using imaging software. A high precision and a low limit of detection were found in the analysis of six standard antioxidants including gallic acid, trolox, ascorbic acid, caffeic acid, vanilliic acid and quercetin. The device was then validated against the traditional spectrophotometric DPPH assay by analyzing the antioxidant activity of 7 tea samples. The results showed no significant difference for gallic acid equivalent for all 7 samples obtained from the two methods at the 95% confidence level, indicating that the developed method was reliable for antioxidant activity analysis of real samples. Finally, the paper-based DPPH device was found to be stable over 10 days when stored in a refrigerator (2 - 4°C), making it an easy-to-use device for end-users.

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Section: Introductionmentioning

confidence: 99%

Paper-based DPPH Assay for Antioxidant Activity Analysis

2018

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“…Nowadays, the spectrophotometric method can be performed by using smartphone systems to reduce expensive cost, easy to use because their operating system is the smallest and portable. [20][21][22][23][24] As well, its camera lenses are high-quality hardware to evaluate colorimetric or light intensity via Complementary Metal Oxide Semiconductor (CMOS). 25,26 For instance, M. Á. Aguirre et al, developed the smartphone application for ibuprofen analysis in commercial tablets.…”

Section: Introductionmentioning

confidence: 99%

A Portable Reflective Absorbance Spectrophotometric Smartphone Device for the Rapid and Highly Accurate Determination of Amlodipine in Pharmaceutical Formulation and Human Urine Samples

Khachornsakkul

Dungchai

2020

ANAL. SCI.

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The simple reflective absorbance spectrophotometric smartphone device for point-ofmonitoring amlodipine is presented here for the first time. The immediate analysis of amlodipine in the human urine of the patients who suffered severe side effects of this drug is very important for diagnosis, treatment, and reduction of the death rate. This measurement technique is based on the charge-transfer complex between amlodipine and picric acid, which forms a yellow product. This product can absorb light intensity from an LED strip and measure through the Blue channel from RGB mode with a smartphone application. The linear relationship for amlodipine monitoring was found in a wide range from 100.0 µg L-1 to 140.0 mg L-1 (R 2 = 0.999), and the limit of detection was found to be 25.0 µg L-1. Our proposed method can be applied to the different smartphone brands with consistent sensitivity of amlodipine detection. Additionally, the determination of amlodipine in pharmaceutical formulation and human urine samples was demonstrated by our proposed method. The recoveries were indicated in the range 98.60-100.00%, which is at the acceptable level for pharmacy. This method offers interweave of basic technology and chemical analysis with environmentally friendly due to reducing the complex instrument and the amount of organic waste compared to chromatographic technique and efficient use for the detection of amlodipine. Hence, this method can be applied for promptly medical diagnoses and laboratories with limited budget resources.

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“…Recently, the use of digital cameras, such as the one from a smartphone, as a detector on green chemical analysis are getting popular due to its availability, portability and low cost [7][8][9][10]. Digital image-based quantitative analysis uses the color change of analyte and reagent that is linearly correlated to the concentration change of interest chemical compound.…”

Section: Introductionmentioning

confidence: 99%

Development of Low-Cost Spectrophotometry Laboratory Practice Based on the Digital Image for Analytical Chemistry Subject

Firdaus¹,

Parlindungan²,

Sundaryono³

et al. 2019

Proceedings of the 3rd Asian Education Symposium (AES 2018)

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A module for laboratory practice in quantitative analytical chemistry subject has been developed. The method uses digital image colorimetry as a basis for low-cost spectrophotometry analysis. Reduction-oxidation reaction of silver nanoparticle and mercury (II) ion was used as a case study for the colorimetric experiment. The yellow color of silver nanoparticle was faded linearly to colorless with the addition of mercury ion as an analyte. The color change was then recorded with a digital camera and processed through computer software to extract the color information in the form of Red, Green and Blue. The module of laboratory practice was then applied to students in chemical education study program. The gain of the learning process was measured using pre-test and post-test. The results of the study indicate that the students were able to understand the concept of spectrophotometry by using the developed module of digital image colorimetry. Therefore, we conclude that laboratory practice of digital image colorimetry is applicable as an alternative to the traditional spectrophotometry.

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Use of a Smartphone as a Colorimetric Analyzer in Paper-based Devices for Sensitive and Selective Determination of Mercury in Water Samples

Cited by 52 publications

References 20 publications

Paper-based DPPH Assay for Antioxidant Activity Analysis

Paper-based DPPH Assay for Antioxidant Activity Analysis

A Portable Reflective Absorbance Spectrophotometric Smartphone Device for the Rapid and Highly Accurate Determination of Amlodipine in Pharmaceutical Formulation and Human Urine Samples

Development of Low-Cost Spectrophotometry Laboratory Practice Based on the Digital Image for Analytical Chemistry Subject

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