2012
DOI: 10.1186/1475-925x-11-92
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Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering

Abstract: BackgroundTissue engineering represents a promising new method for treating heart valve diseases. The aim of this study was evaluate the importance of conditioning procedures of tissue engineered polyurethane heart valve prostheses by the comparison of static and dynamic cultivation methods.MethodsHuman vascular endothelial cells (ECs) and fibroblasts (FBs) were obtained from saphenous vein segments. Polyurethane scaffolds (n = 10) were primarily seeded with FBs and subsequently with ECs, followed by different… Show more

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Cited by 24 publications
(15 citation statements)
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“…After one week of implantation in utero, scaffolds demonstrated cellular integration characterized by increased cellularity and glycosaminoglycan (GAG) content . Furthermore, human fibroblasts and endothelial cells isolated from saphenous vein were used to seed a polyurethane nanofibrous scaffold . The fibroblasts were able to spread on the fibrous scaffold, while the endothelial cells formed a monolayer over the fibroblasts.…”
Section: Design Considerations For Heart Valve Tissue Engineeringmentioning
confidence: 99%
See 1 more Smart Citation
“…After one week of implantation in utero, scaffolds demonstrated cellular integration characterized by increased cellularity and glycosaminoglycan (GAG) content . Furthermore, human fibroblasts and endothelial cells isolated from saphenous vein were used to seed a polyurethane nanofibrous scaffold . The fibroblasts were able to spread on the fibrous scaffold, while the endothelial cells formed a monolayer over the fibroblasts.…”
Section: Design Considerations For Heart Valve Tissue Engineeringmentioning
confidence: 99%
“…The fibroblasts were able to spread on the fibrous scaffold, while the endothelial cells formed a monolayer over the fibroblasts. The cells responded better to a dynamic culture setting, showing more proliferation and ECM production . Although these studies are promising, the direct application of primary cells for TEHVs may not be suitable for clinical translation, as allogenic cells can induce an immune response in the recipient of the TEHV .…”
Section: Design Considerations For Heart Valve Tissue Engineeringmentioning
confidence: 99%
“…This tissue construct could be a tissue model for valve regeneration. Instead of PGA, polyurethane fibrous scaffolds were used to generate a similar tissue construct that contained both fibroblasts and ECs (Aleksieva et al, 2012). Furthermore, instead of applying a static culture, as was done previously, this experiment implemented both dynamic and static conditioning.…”
Section: Fibroblastsmentioning
confidence: 99%
“…FBs and ECs were phenotypically characterized by morphological observation and immunocytochemical staining (ICC) in eight-well-chamber slides (BD Bioscience, Franklin Lakes, NJ, USA) using specific antibodies as previously described [49]. Briefly, vascular cells were stained against EC-specific CD31 (10.25 μg/mL; Dako Deutschland GmbH, Hamburg, Germany) and FB-specific TE-7 (2 μg/mL, Millipore Corporation BioScience Division, Temecula, CA, USA), respectively, according to manufacturer's protocol using EnVision ™ + Dual Link System-HRP (Dako Deutschland GmbH, Hamburg, Germany).…”
Section: Cell Characterizationmentioning
confidence: 99%