Use of a synthetic adjuvant in an effective vaccination of monkeys against malaria

Merozoite surface coat precursor protein completely protects Aotus monkeys against Plasmodium falciparum malaria.

“…After an increased prepatent period, parasitemias in all three monkeys rose to about 2%. Although the degree of protection seen in these monkeys was less than that of the merozoite surface-coat precursor protein-immunized group, it was similar to the results obtained from our previous successful vaccination experiments using whole parasites as immunogen (33)(34)(35). We have found that the 143-, 132-, and 102-kDa proteins aggregate with the merozoite surface-coat precursor protein when the mixtures were iodinated (unpublished data).…”

Enhancement of delayed-type hypersensitivity and induction of interferon by the lipophilic agents DDA and CP-20,961

“…The observed protection was probably mediated by interferon, which was detected in plasma after administration of the drug. Later it was shown by the same and other authors that CP-20,961 also has immunostimulatory properties (6)(7)(8).…”

“…Fourteen mice were injected ip with 1500 pg (75 mg/kg) DDA each. At different intervals (4,6,8,12,14, and 16 hr after injection) blood, peritoneal fluid, and PEC were collected. PEC were incubated in Eagle's medium without serum for 24 hr at 37°C and the supematants were also titrated for the presence of interferon.…”

Rhoptry-Associated Protein 1-Binding Monoclonal Antibody Raised against a Heterologous Peptide Sequence InhibitsPlasmodium falciparumGrowth In Vitro

“…Aotus monkeys, which are susceptible to infection with P. falciparum (8,9,17,27,34,35,39,41,(44)(45)(46)(47)58) without prior splenectomy (48), are a World Health Organizationrecommended model to test the efficacy of malaria blood stage vaccine candidates. An evaluation of the protective potential of a series of peptide sequences derived from proteins isolated from P. falciparum-infected erythrocytes in the Aotus infection model identified the partially protective peptides 35.1, 55.1, and 83.1 (34).…”