2019
DOI: 10.1111/ejh.13263
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Use of a whole‐cell ELISA to detect additional antibodies in setting of suspected heparin‐induced thrombocytopenia

Abstract: Objectives: Type II heparin-induced thrombocytopenia (HIT) is mediated by formation of antibodies to platelet factor 4 (PF4)-heparin complexes. We evaluated anti-PF4heparin-negative samples for the presence of additional anti-platelet and anti-red blood cell (RBC) antibodies using whole-cell platelet/ RBC ELISAs we developed. Methods: Seventy-three samples tested for anti-PF4-heparin by ELISA were included: 62 tested negative, 9 tested positive, and 2 had equivocal results. Plasma specimens from healthy donors… Show more

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“…Bashover et al recently conducted a study on anti-PF4-heparin-negative samples by using whole-cell ELISA. 51 They detected that 12.5% (eight of 64) of negative samples were found positive for antibodies against antigens on platelets or red blood cells.…”
Section: Enzyme-linked Immunosorbent Assaysmentioning
confidence: 98%
“…Bashover et al recently conducted a study on anti-PF4-heparin-negative samples by using whole-cell ELISA. 51 They detected that 12.5% (eight of 64) of negative samples were found positive for antibodies against antigens on platelets or red blood cells.…”
Section: Enzyme-linked Immunosorbent Assaysmentioning
confidence: 98%