Use of Acetylcholinesterase in Perna perna and Mytilus galloprovincialis as a Biomarker of Pollution in Agadir Marine Bay (South of Morocco)

Najimi, S.; Bouhaimi, Abdellah; Daubèze, M.; Zekhnini, A.; Pellerin, J.; Narbonne, J.; Moukrim, Abdellatif

doi:10.1007/s001289900419

Cited by 152 publications

(62 citation statements)

References 14 publications

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“…Heavy metals such as mercury and copper are capable of inhibiting ChE by interacting with the negatively charged amino acids such as aspartate or imidazole group of histidine, or cleavage of disulfide bond that leads structural changes (Masson et al, 1996;Najimi et al, 1997;Abdelhamid et al, 2007;Frasco et al, 2008). Mercury and copper both caused low inhibition of unpurified ChE due to their capability to bind with other thiol and amino groups in protein (Fig.…”

Section: Discussionmentioning

confidence: 99%

Purification and Anticholinesterase Sensitivity of Cholinesterase Extracted from Liver Tissue of Puntius Javanicus

Sabullah¹,

Shukor²,

Shamaan³

et al. 2015

IJAB

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The purification of a soluble cholinesterase (ChE) from Puntius javanicus liver using affinity chromatography was studied. Affinity matrix was synthesised through the cooling system of ligands procainamide to epoxy-activated Sephacryl 6B and purification process was performed using calibrated flow rate at 0.2 mL/min. Non-denaturing electrophoresis condition was employed and the single band native form of ChE was detected at 66.267 kDa after being stained with commasie brilliant blue. ChE detection was performed using gel filtration; ZORBAX column attached to the HPLC with the flow rate of 1 mL/min. Only a single peak was detected at the retention time of 3.720. From the assay evaluation, the final purified ChE procedure displayed the highest sensitivity of detecting the anticholinesterase namely mercury, copper, malaoxon and carbofuran compared to the impure ChE and the results were further discussed in detail to the potential application of ChE from P. javanicus as a biomarker for those toxicants.

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Section: Discussionmentioning

confidence: 99%

Purification and Anticholinesterase Sensitivity of Cholinesterase Extracted from Liver Tissue of Puntius Javanicus

Sabullah¹,

Shukor²,

Shamaan³

et al. 2015

IJAB

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“…AChE activity has been widely used as a biomarker of exposure to organophosphate and carbamate pesticides in several field studies (Escartı´n and Porte, 1997;Radenac et al, 1998). Recently, several works evidence that other types of contaminants such as metals, surfactants (Martinez-Tabche et al, 1997;Najimi et al, 1997;Guilhermino et al, 1998) and compounds in complex mixtures (Payne at al., 1996) may also inhibit AChE activity. Moreover, several laboratory results supported the anticholinesterase effect of petroleum and PAH-type compounds (Kang and Fang, 1997;Martinez-Tabche et al, 1997;Akcha et al, 2000), although the mechanisms of this inhibition are not clear.…”

Section: Discussionmentioning

confidence: 99%

The ?Coral Bulker? Fuel Oil Spill on the North Coast of Portugal: Spatial and Temporal Biomarker Responses in Mytilus galloprovincialis

et al. 2004

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Abstract. In December 2000, the ship 'Coral Bulker' ran aground at the entrance of the port of Viana do Castelo (North-west coast of Portugal). A large amount of fuel oil was spilled and part of it reached the shore. To evaluate the spatial and temporal impact of this oil spill, a field study, and several laboratory toxicity tests were performed using Mytilus galloprovincialis as biological indicator of environmental contamination and the biomarkers glutathione S-transferases (GSTs) and acetylcholinesterase (AChE) as indicative criteria. Fifteen days after the oil spill, mussels collected at stations located near the ship presented higher and lower values of GSTs and AChE activity, respectively. These results, and those obtained in the laboratory toxicity tests, evidence that these biomarkers were sensitive indicators of exposure to this kind of pollution and were able to monitor a spatial impact of the oil spill of at least 10 km, confirming the higher level of contamination near the ship and a contamination gradient along the sampling stations. One year after the accident, such a contamination gradient was no longer evident. This study highlight the potential suitability of a biomarker approach for assessing spatial and temporal impacts of marine pollution accidents, such as fuel oil spills, suggesting the inclusion of these biomarkers in risk assessment studies, as cost-effective and early warning recognized tools. Major advantages and limitations of the biomarker approach used in this study are further discussed.

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“…Figure 4 showed that AChE activity measured in gills was unaffected across effluent contamination. The ROS, which results from the Cd exposure, alter the structure of the cell membranes by stimulating the lipid peroxidation process (Harris, 1992 Monitoring studies of neurotoxic compounds are mostly based on the inhibition of AChE activity, the majority relating this response to a contamination by organophosphorous, carbamates and heavy metals (Najimi et al 1997 …”

Section: Biomarker Responsesmentioning

confidence: 99%

Effect of in Vivo Contamination by Effluent From Phosphate Treatment Industry on the Clam Ruditapes Decussatus

Chaabouni¹

2017

SDRP-JESES

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This study focused on industrial effluent characterization and determination of its effects on the clam Ruditapes decussatus.The analysis results revealed that the effluent having acid pH and high concentrations of some chemicals, comparing to the norms, such as SO4 2-, NO3 -, K + , Mg 2+ , F and Cd. The effect of the effluent on the clam R. decussatus was reflected by the LT50 determined after an exposition to different doses (5%, 10%, 20%, 30%) during 25 days and by the concentration/activity of two biomarkers: malondialdehyde (MDA) and acetylcholinesterase (AChE) determined after a contamination by effluent (1%) during 16 days. The stress on stress results showed that animals were stressed even with the lowest effluent concentration. An increase of MDA concentrations was observed in the digestive glands of exposed clams. Nevertheless, no significant variation of AChE activity was observed.

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Use of Acetylcholinesterase in Perna perna and Mytilus galloprovincialis as a Biomarker of Pollution in Agadir Marine Bay (South of Morocco)

Cited by 152 publications

References 14 publications

Purification and Anticholinesterase Sensitivity of Cholinesterase Extracted from Liver Tissue of Puntius Javanicus

Purification and Anticholinesterase Sensitivity of Cholinesterase Extracted from Liver Tissue of Puntius Javanicus

The ?Coral Bulker? Fuel Oil Spill on the North Coast of Portugal: Spatial and Temporal Biomarker Responses in Mytilus galloprovincialis

Effect of in Vivo Contamination by Effluent From Phosphate Treatment Industry on the Clam Ruditapes Decussatus

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