“Use of acidophilic bacteria of the genus Acidithiobacillus to biosynthesize CdS fluorescent nanoparticles (quantum dots) with high tolerance to acidic pH”

Ulloa, Giovanni; Collao, Bernardo; Araneda, Mabel; Escobar, Beatriz; Álvarez, Sergio A.; Bravo, Daniela; Pérez-Donoso, José M.

doi:10.1016/j.enzmictec.2016.09.005

Cited by 51 publications

(74 citation statements)

References 38 publications

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“…The capacity to produce nanoparticles has been reported in distantly related bacteria such as Escherichia coli, Bacillus mycoides , and Rhodopseudomonas capsulatus (He et al, 2007 ; Iravani, 2014 ; Ordenes-Aenishanslins et al, 2014 ; Jacob et al, 2016 ). Biosynthesis of QDs has also been observed in bacterial cells with high metal resistance, increased tolerance to oxidative stress or overexpressing genes involved in thiol metabolism (Narayanan and Sakthivel, 2010 ; Valizadeh et al, 2012 ; Dunleavy et al, 2016 ; Ulloa et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Recently, we reported for the first time the production of CdS QDs in acidophilic cells (Ulloa et al, 2016 ). Quantum dots biosynthesis by extremophile bacteria belonging to the genus Acidithiobacillus was associated to Cd tolerance in a process favored by acidic pH (Ulloa et al, 2016 ). These QDs presented high stability at acidic pH and displayed diverse fluorescence properties, offering advantages to those produced by non-acidophilic bacteria or by chemical methods (Ulloa et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, the production of QDs by Acidothiobacillus requires biological thiols like cysteine and glutathione for H 2 S generation (Ulloa et al, 2016 ). These antioxidant thiols have been previously described as precursors for Cd QDs in bacteria and fungus (Dameron et al, 1989 ; Bai et al, 2009 ; Valizadeh et al, 2012 ; Gallardo et al, 2014 ; Plaza et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Phosphate Favors the Biosynthesis of CdS Quantum Dots in Acidithiobacillus thiooxidans ATCC 19703 by Improving Metal Uptake and Tolerance

et al. 2018

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Recently, we reported the production of Cadmium sulfide (CdS) fluorescent semiconductor nanoparticles (quantum dots, QDs) by acidophilic bacteria of the Acidithiobacillus genus. Here, we report that the addition of inorganic phosphate to Acidithiobacillus thiooxidans ATCC 19703 cultures favors the biosynthesis of CdS QDs at acidic conditions (pH 3.5). The effect of pH, phosphate and cadmium concentrations on QDs biosynthesis was studied by using Response Surface Methodology (RSM), a multivariate technique for analytical optimization scarcely used in microbiological studies to date. To address how phosphate affects intracellular biosynthesis of CdS QDs, the effect of inorganic phosphate on bacterial cadmium-uptake was evaluated. By measuring intracellular levels of cadmium we determined that phosphate influences the capacity of cells to incorporate this metal. A relation between cadmium tolerance and phosphate concentrations was also determined, suggesting that phosphate participates in the adaptation of bacteria to toxic levels of this metal. In addition, QDs-biosynthesis was also favored by the degradation of intracellular polyphosphates. Altogether, our results indicate that phosphate contributes to A. thiooxidans CdS QDs biosynthesis by influencing cadmium uptake and cadmium tolerance. These QDs may also be acting as a nucleation point for QDs formation at acidic pH. This is the first study reporting the effect of phosphates on QDs biosynthesis and describes a new cadmium-response pathway present in A. thiooxidans and most probably in other bacterial species.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phosphate Favors the Biosynthesis of CdS Quantum Dots in Acidithiobacillus thiooxidans ATCC 19703 by Improving Metal Uptake and Tolerance

et al. 2018

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“…TEM images showed a uniform distribution of CdS QDs with an average diameter of 0.25 ± 8.01 nm. The acidophilic bacterium of the Acidithiobacillus genus mediated the synthesis of CdS QDs [ 101 ]. Three Acidithiobacillus species, namely A. thiooxidans , A. caldus , and A. ferrooxidans , were exposed to sub-lethal concentrations of Cd 2+ in presence of glutathione and cysteine.…”

Section: Microbial Synthesis Of Qdsmentioning

confidence: 99%

Superior Properties and Biomedical Applications of Microorganism-Derived Fluorescent Quantum Dots

et al. 2020

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Quantum dots (QDs) are fluorescent nanocrystals with superb photo-physical properties. Applications of QDs have been exponentially increased during the past decade. They can be employed in several disciplines, including biological, optical, biomedical, engineering, and energy applications. This review highlights the structural composition and distinctive features of QDs, such as resistance to photo-bleaching, wide range of excitations, and size-dependent light emission features. Physical and chemical preparation of QDs have prominent downsides, including high costs, regeneration of hazardous byproducts, and use of external noxious chemicals for capping and stabilization purposes. To eliminate the demerits of these methods, an emphasis on the latest progress of microbial synthesis of QDs by bacteria, yeast, and fungi is introduced. Some of the biomedical applications of QDs are overviewed as well, such as tumor and microRNA detection, drug delivery, photodynamic therapy, and microbial labeling. Challenges facing the microbial fabrication of QDs are discussed with the future prospects to fully maximize the yield of QDs by elucidating the key enzymes intermediating the nucleation and growth of QDs. Exploration of the distribution and mode of action of QDs is required to promote their biomedical applications.

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Living Materials Based Dynamic Information Encryption via Light‐Inducible Bacterial Biosynthesis of Quantum Dots

Niu,

Yu,

Jin

et al. 2023

Angewandte Chemie

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Microbial biosynthesis, as an alternative method for producing quantum dots (QDs), has gained attention because it can be conducted under mild and environmentally friendly conditions, distinguishing it from conventional chemical and physical synthesis approaches. However, there is currently no method to selectively control this biosynthesis process in a subset of microbes within a population using external stimuli. In this study, we have attained precise and selective control over the microbial biosynthesis of QDs through the utilization of an optogenetically engineered Escherichia coli (E. coli). The recombinant E. coli is designed to express smCSE enzyme, under the regulation of eLightOn system, which can be activated by blue light. The smCSE enzymes use L‐cysteine and Cd2+ as substrates to form CdS QDs. This system enables light‐inducible bacterial biosynthesis of QDs in precise patterns within a hydrogel for information encryption. As the biosynthesis progresses, the optical characteristics of the QDs change, allowing living materials containing the recombinant E. coli to display time‐dependent patterns that self‐destruct after reading. Compared to static encryption using fluorescent QD inks, dynamic information encryption based on living materials offers enhanced security.

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“Use of acidophilic bacteria of the genus Acidithiobacillus to biosynthesize CdS fluorescent nanoparticles (quantum dots) with high tolerance to acidic pH”

Cited by 51 publications

References 38 publications

Phosphate Favors the Biosynthesis of CdS Quantum Dots in Acidithiobacillus thiooxidans ATCC 19703 by Improving Metal Uptake and Tolerance

Phosphate Favors the Biosynthesis of CdS Quantum Dots in Acidithiobacillus thiooxidans ATCC 19703 by Improving Metal Uptake and Tolerance

Superior Properties and Biomedical Applications of Microorganism-Derived Fluorescent Quantum Dots

Living Materials Based Dynamic Information Encryption via Light‐Inducible Bacterial Biosynthesis of Quantum Dots

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