Use of activated charcoal to trap gas chromatographic fractions for mass spectrometric analysis and to introduce volatile compounds into the mass spectrometer

Damico, Joseph N.; Wong, N.P.; Sphon, James A.

doi:10.1021/ac60252a041

Cited by 21 publications

(10 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The resulting spectrum is given in the top panel of Figure 1 and is in agreement with the spectrum reported by Damico (1966). The molecular ion is not observed.…”

Section: Resultssupporting

confidence: 86%

See 1 more Smart Citation

Comparison of high-performance liquid chromatography and anticholinesterase assay for measuring azinphos-methyl metabolism in vitro

Lin¹,

Caprioli²,

Murphy³

1983

J. Agric. Food Chem.

View full text Add to dashboard Cite

The oxon of azinphos-methyl was not thermally stable. Under gas chromatographic conditions, on-column decomposition occurred and tended to produce components which upon electron impact generated the same ions as those generally employed to identify the metabolites of azinphos-methyl by mass spectrometry. Furthermore, approximately 44 % of the oxon of azinphos-methyl generated enzymatically by mice liver homogenates was decomposed to benzazimide if the incubation mixture was heated to 100 "C for 3 min. Therefore, it was concluded that analytical methods for studying the metabolism of azinphos-methyl should not involve processes which required high temperature. Investigation of the interchangeability between high-performance liquid chromatography (HPLC) and the anticholinesterase assay (ACHE) was carried out by quantitating the oxon of azinphos-methyl in the same biological samples. Both methods selectively measured the compound to the same degree of accuracy. Whereas the ACHE assay appeared to be faster, HPLC was advantageous in that several metabolites of azinphos-methyl could be simultaneously assayed.

show abstract

“…The resulting spectrum is given in the top panel of Figure 1 and is in agreement with the spectrum reported by Damico (1966). The molecular ion is not observed.…”

Section: Resultssupporting

confidence: 86%

“…However, m/z 160 corresponds to an ion produced by a-cleavage of the S-C bond and further elimination of CO to form m/z 132. The ion at m/z 109 has an ionic structure of [(CH3-O),P=O]+ (Damico, 1966;Casida, 1974). The mass spectrum of the oxon of azinphos-methyl after GC separation is shown on the lower panel of Figure 1.…”

Section: Resultsmentioning

confidence: 99%

Comparison of high-performance liquid chromatography and anticholinesterase assay for measuring azinphos-methyl metabolism in vitro

Lin¹,

Caprioli²,

Murphy³

1983

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Several specialized techniques are available for efficient trapping and collection of individual gas chromatographic peaks for subsequent mass spectral analysis by the direct probe introduction method (Amy et al, 1965;Damico et al, 1967;Muller, 1963;Mumma and Kantner, 1966). These methods, although practical and reliable, are time-consuming and may risk sample loss by oxidation or hydrolysis.…”

mentioning

confidence: 99%

Pesticide residue analysis in human tissue by combined gas chromatography---mass spectrometry

Biros¹,

Walker²

1970

J. Agric. Food Chem.

View full text Add to dashboard Cite

Pesticide residues in human adipose tissue and liver tissue samples were identified by mass spectrometry coupled with gas chromatography. An extensive extraction and cleanup procedure was adapted from existing analytical methods to separate pesticide residues from the tissue samples. Concentration of pesticides in tissue ranged from 0.073 to 28.7 ppm. Mass spectral data were computer-enhanced to identify heptachlor epoxide. Other pesticides confirmed included the /3-isomer of 1,2,3,4,5,6-hexachlorocyclohexane (HCH), several isomers and metabolites of , '-DOT, and dieldrin. Advantages, limitations, and suggested modifications for improving the combined gas chromatographymass spectrometry technique for pesticide residue analysis are discussed.

show abstract

“…Klirnes et a/ [!jl have evaluated recovery efficiencies thoroughly and found that these depend on gas flow, temperature of the coolant, trap design and boiling point of the substance collected. While trapping often involves the use of materials like glass wool [13], Preparative GC with Glass Capillaries adsorbents [14] or stationary phases [15], the aim in this study was to achieve maximum trapping efficiency without the use of foreign materials. Therefore, the traps were made up of several coils of glass capillary tubing, which had internal diameters similar to or somewhat larger than that of the glass capillary column employed.…”

Section: Trappingmentioning

confidence: 99%

Preparative gas chromatography with glass capillary columns

Roeraade¹,

Enzell²

1979

J. High Resol. Chromatogr.

View full text Add to dashboard Cite

SummaryThe present paper describes an automated system for preparative gas chromatography with glass capillary columns, controlled by a microprocessor. The effluent from the capillary column is divided by a pneumatically controlled splitter and any desired split ratio between traps and detector can be obtained. Moreover, a second pneumatic control allows instantaneous change-over to a different split ratio, thus minimizing loss of material during collection.The effluent containing the compounds of interest is passed through a multiple manifold and collected in coiled glass capillary traps. To ensure maximum trapping efficiency even for very small amounts of material, the inner walls of the capillary traps are wetted with a suitable solvent, which gives a quantitative recovery of micro-and nanogram amounts of material.After repetitive sampling, sufficient amounts of material can be obtained for NMR spectroscopy and possibilities exist to enrich tracecomponentswith theaidof adoublecolumnsystem. Two examples of such applications are given, employing mixtures of both synthetic and natural origin.

show abstract

Use of activated charcoal to trap gas chromatographic fractions for mass spectrometric analysis and to introduce volatile compounds into the mass spectrometer

Cited by 21 publications

References 5 publications

Comparison of high-performance liquid chromatography and anticholinesterase assay for measuring azinphos-methyl metabolism in vitro

Comparison of high-performance liquid chromatography and anticholinesterase assay for measuring azinphos-methyl metabolism in vitro

Pesticide residue analysis in human tissue by combined gas chromatography---mass spectrometry

Preparative gas chromatography with glass capillary columns

Contact Info

Product

Resources

About