Use of ADME studies to confirm the safety of ε-polylysine as a preservative in food

Hiraki, Jun; Ichikawa, Takafumi; Ninomiya, Shinichi; Seki, Hideaki; Katsumi, Uohama; Seki, Hiroshi; Kimura, Shigemi; Yanagimoto, Yukio; Barnett, James W.

doi:10.1016/s0273-2300(03)00029-1

Cited by 270 publications

(149 citation statements)

References 3 publications

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“…In this study, we report the in vivo influence on the microbiome by a food grade antimicrobial ε-polylysine. Both ε-polylysine and pectin are not hydrolyzed or absorbed in the upper GIT, 36,37 thus have the opportunity to interact with resident microbial communities established along the full length of the GIT.…”

Section: Discussionmentioning

confidence: 99%

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Food-grade cationic antimicrobial ε-polylysine transiently alters the gut microbial community and predicted metagenome function in CD-1 mice

et al. 2017

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Diet is an important factor influencing the composition and function of the gut microbiome, but the effect of antimicrobial agents present within foods is currently not understood. In this study, we investigated the effect of the food-grade cationic antimicrobial ε-polylysine on the gut microbiome structure and predicted metagenomic function in a mouse model. The relative abundances of predominant phyla and genera, as well as the overall community structure, were perturbed in response to the incorporation of dietary ε-polylysine. Unexpectedly, this modification to the gut microbiome was experienced transiently and resolved to the initial basal composition at the final sampling point. In addition, a differential non-random assembly was observed in the microbiomes characterized from male and female co-housed animals, although their perturbation trajectories in response to diet remain consistent. In conclusion, antimicrobial ε-polylysine incorporated into food systems transiently alters gut microbial communities in mice, as well as their predicted function. This indicates a dynamic but resilient microbiome that adapts to microbial-active dietary components.

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Section: Discussionmentioning

confidence: 99%

“…36,37 Consequently, ε-polylysine or ε-polylysine-pectin complexes would be expected to reach the colon intact and thus available to interact with the gut microbial community. In this study, the antimicrobial food additive ε-polylysine was experimentally investigated to characterize potential perturbations to the gut microbiome.…”

Section: Introductionmentioning

confidence: 99%

Food-grade cationic antimicrobial ε-polylysine transiently alters the gut microbial community and predicted metagenome function in CD-1 mice

et al. 2017

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“…The native e-PLL is recognized as a very safe substance in the scientific community, which has been approved by FDA for use as an antimicrobial agent at levels up to 50 mg/kg of cooked rice (Hiraki et al, 2003). The results of an acute toxicity study show that there was no toxicity in reproduction, neurological and immunological functions, embryonic and fetal development, growth offspring and development of embryos, etc.…”

Section: In Vitro Cytotoxicity Studiesmentioning

confidence: 99%

Polymeric lipid vesicles with pH-responsive turning on–off membrane for programed delivery of insulin in GI tract

Fang

Xue

et al. 2016

Drug Delivery

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A kind of polymeric lipid vesicles (PLVs) with pH-responsive turning on-off membrane for programed delivery of insulin in gastrointestinal (GI) tract was developed, which was selfassembled from the grafted amphipathic polymer of N-tocopheryl-N 0 -succinyl-e-poly-L-lysine (TP/SC-g-PLL). By controlling the grafting ratio of hydrophobic alkane and ionizable carboxyl branches, the permeability of membrane was adjustable and thus allowing insulin release in a GI-pH dependent manner. The effects of grafting degree of substitution (DS) on the pHresponsive behavior of the formed vesicles were confirmed by critical aggregation concentration determination, morphology and size characterization. Their transepithelial permeability across the GI tract was proved by both confocal visualization in vitro model of Caco-2 cellular monolayer and in vivo hypoglycemic study in diabetic rats. Accordingly, the work described here indicated that the self-assembled PLVs could be a promising candidate for improving the GI delivery of hydrophilic biomacromolecule agents.

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“…ε-PL shows a wide range of antimicrobial activity and is stable at high temperatures and under both acidic and alkaline conditions. As a kind of natural food preservative, ε-PL become more and more popular for its natural, innocuous and antimicrobial property [7,8]. Bacterial cellulose is an interesting material for active packaging films.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Antimicrobial Activity Bacterial Cellulose Membrane

Tang¹,

Jia²,

Jia³

2016

Proceedings of the 2016 International Conference on Biological Sciences and Technology

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Abstract.Bacterial cellulose (BC) shows good potentiality as active packaging material. However, bacterial cellulose itself has no antimicrobial activity to prevent microbial contamination. To achieve antimicrobial activity, ε-poly lysine (ε-PL) was incorporated into BC by immersing BC in ε-PL solution. Investigations into the effects of ε-PL concentrations and contact times on incorporation of ε-PL into cellulose membranes showed that the lowest ε-PL concentration and the shortest time for production of an effective antimicrobial cellulose membrane (ε-PL/BC) were 400 mg/l and 2 hours, respectively. The antimicrobial cellulose membrane was characterized by FTIR, XRD and AFM. It was found that ε-PL is incorporated into the BC network, and the antimicrobial cellulose membrane is not a simple mixture of BC and ε-PL. In this study ε-PL/BC membranes are demonstrated to have potential applicability as antimicrobial packaging films.

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Use of ADME studies to confirm the safety of ε-polylysine as a preservative in food

Cited by 270 publications

References 3 publications

Food-grade cationic antimicrobial ε-polylysine transiently alters the gut microbial community and predicted metagenome function in CD-1 mice

Food-grade cationic antimicrobial ε-polylysine transiently alters the gut microbial community and predicted metagenome function in CD-1 mice

Polymeric lipid vesicles with pH-responsive turning on–off membrane for programed delivery of insulin in GI tract

Preparation and Characterization of Antimicrobial Activity Bacterial Cellulose Membrane

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