Use of almond mesocarp for production of the entomopathogenic fungus <i>Verticillium lecanii</i>

López-Llorca, Luis Vicente; Carbonell, T.

doi:10.1139/w98-076

Cited by 8 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thereafter, fungal cultures were adjusted photometrically (absorbance at 530 nm; McFarland 0.5 standard) to a concentration of 1 ϫ 10 6 to 5 ϫ 10 6 cells/ml. In the case of filamentous fungi, conidia were separated from the rest of the mycelium by filtration through sterile glass wool (28). Tenfold serial dilutions of Candida albicans and Aspergillus fumigatus (10 6 to 10 0 cells) were prepared to test the sensitivity and specificity of the assay.…”

Section: Standardmentioning

confidence: 99%

Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5.8S Ribosomal DNA Typing in Ocular Infections

Ferrer

Colom²,

Frasés³

et al. 2001

J Clin Microbiol

311

197

View full text Add to dashboard Cite

The goal of this study was to determine whether sequence analysis of internal transcribed spacer/5.8S ribosomal DNA (rDNA) can be used to detect fungal pathogens in patients with ocular infections (endophthalmitis and keratitis). Internal transcribed spacer 1 (ITS1) and ITS2 and 5.8S rDNA were amplified by PCR and seminested PCR to detect fungal DNA. Fifty strains of 12 fungal species (yeasts and molds) were used to test the selected primers and conditions of the PCR. PCR and seminested PCR of this region were carried out to evaluate the sensitivity and specificity of the method. It proved possible to amplify the ITS2/5.8S region of all the fungal strains by this PCR method. All negative controls (human and bacterial DNA) were PCR negative. The sensitivity of the seminested PCR amplification reaction by DNA dilutions was 1 organism per PCR, and the sensitivity by cell dilutions was fewer than 10 organisms per PCR. Intraocular sampling or corneal scraping was undertaken for all patients with suspected infectious endophthalmitis or keratitis (nonherpetic), respectively, between November 1999 and February 2001. PCRs were subsequently performed with 11 ocular samples. The amplified DNA was sequenced, and aligned against sequences in GenBank at the National Institutes of Health. The results were PCR positive for fungal primers for three corneal scrapings, one aqueous sample, and one vitreous sample; one of them was negative by culture. Molecular fungal identification was successful in all cases. Bacterial detection by PCR was positive for three aqueous samples and one vitreous sample; one of these was negative by culture. Amplification of ITS2/5.8S rDNA and molecular typing shows potential as a rapid technique for identifying fungi in ocular samples.The microbiological spectrum of infectious endophthalmitis shows that the percentage of isolates that are fungi is 8 to 18.5% (2,7,12,22,23) and in keratitis the rate is 16 to 35.9% (8,42). Clinical diagnosis of these ocular infections is confirmed by obtaining intraocular (aqueous or vitreous) specimens or corneal scrapings. However, standard microbiological tests are positive in only 54 to 69% of endophthalmitis cases (13,22,23) (by culture) and 80% (8) of keratitis cases (by Gram and Giemsa stains and culture). In fungal infections, even when positive, results usually take longer than a week because these organisms are difficult to identify and/or are slow-growing. Early diagnosis and rapid intervention is a critical element for an effective treatment of ocular infections. This has led to the development of culture-independent diagnostic tests such as PCR. PCR-based detection methods with universal primers for bacterial DNA in ocular samples (5,16,20,21,26,27,34,36,40) have recently been developed. For detection of fungal pathogens, multicopy gene targets have been evaluated for increasing the sensitivity (33, 39) and universal fungal PCR primers have been developed for broadening the range of detectable fungi (9,14,18,31,37). Studies on fungal DNA detection in ocular ...

show abstract

Section: Standardmentioning

confidence: 99%

Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5.8S Ribosomal DNA Typing in Ocular Infections

Ferrer

Colom²,

Frasés³

et al. 2001

J Clin Microbiol

311

197

View full text Add to dashboard Cite

show abstract

“…Our group has investigated the use of agricultural by-products for the production of entomopathogenic fungi (Lopez-Llorca & Carbonell 1998). We have developed palm by-products for the production of B. bassiana (Asensio et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Use of a Solid Formulation ofBeauveria bassianafor Biocontrol of the Red Palm Weevil (Rhynchophorus ferrugineus) (Coleoptera: Dryophthoridae) Under Field Conditions in SE Spain^*

Güerri-Agulló

López-Follana

Asensio

et al. 2011

Florida Entomologist

View full text Add to dashboard Cite

“…In the case of filamentous fungi, the conidia were separated from the rest of the mycelium by filtration through sterile glass wool. 26 The fungal suspensions with predetermined concentrations were centrifuged at 5000 × g , and then the pellet was frozen at −20°C for 1 hour and incubated at 65°C for 1 hour in 0.5 ml of extraction buffer (50 mM Tris-HCl, 50 mM Ethylenediamine tetraacetic acid, 3% sodium dodecyl sulfate, 1% 2-mercaptoethanol). The lysate was extracted with phenol-chloroform-isoamyl alcohol (25:24:1, vol/vol/vol).…”

Section: Methodsmentioning

confidence: 99%

Treponema spp. spirochetes and keratinopathogenic fungi isolated from keratomas in donkeys

Paraschou

Cook²,

Priestnall

et al. 2022

Vet Pathol

View full text Add to dashboard Cite

Keratoma is an aberrant keratin mass thought to originate from epidermal horn-producing cells interposed between the stratum medium of the hoof wall and the underlying third phalanx. The cause is unknown, although the presence of keratomas is frequently associated with chronic irritation, focal infection, or trauma. A total of 167 donkeys with keratomas were presented in this study. The diagnosis of a keratoma was based on clinical signs, radiography, and histopathologic examination. Surgical excision was attempted on all donkeys with lameness unless euthanasia was advised. Histopathologic examination, including Giemsa, periodic acid Schiff, and Young’s silver special histochemical stains, was performed and showed the presence of fungal hyphae and spirochete bacteria within the degenerate keratin. Polymerase chain reaction (PCR) for treponeme bacteria was performed on 10 keratoma lesions and 9 healthy pieces of hoof (controls). All healthy donkey tissues were negative for the 3 recognized digital dermatitis (DD) treponeme phylogroups, whereas 3 of 10 (30%) donkey keratoma samples were positive for one of the DD treponeme phylogroups. Routine fungal culture and PCR for fungi were performed on 8 keratoma lesions and 8 healthy pieces of hoof (controls). Keratinopathogenic fungi were detected in 1 of 8 (12.5%) keratomas, while only non-keratinopathogenic, environmental fungi were detected in 8 control healthy hoof samples. This is the first time the DD treponemes phylogroup and keratinopathogenic fungi have been detected in keratomas. Further studies are required to assess the significance of this finding.

show abstract

Use of almond mesocarp for production of the entomopathogenic fungus Verticillium lecanii

Cited by 8 publications

References 20 publications

Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5.8S Ribosomal DNA Typing in Ocular Infections

Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5.8S Ribosomal DNA Typing in Ocular Infections

Use of a Solid Formulation ofBeauveria bassianafor Biocontrol of the Red Palm Weevil (Rhynchophorus ferrugineus) (Coleoptera: Dryophthoridae) Under Field Conditions in SE Spain^*

Treponema spp. spirochetes and keratinopathogenic fungi isolated from keratomas in donkeys

Contact Info

Product

Resources

About

Use of almond mesocarp for production of the entomopathogenic fungus Verticillium lecanii

Cited by 8 publications

References 20 publications

Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5.8S Ribosomal DNA Typing in Ocular Infections

Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5.8S Ribosomal DNA Typing in Ocular Infections

Use of a Solid Formulation ofBeauveria bassianafor Biocontrol of the Red Palm Weevil (Rhynchophorus ferrugineus) (Coleoptera: Dryophthoridae) Under Field Conditions in SE Spain*

Treponema spp. spirochetes and keratinopathogenic fungi isolated from keratomas in donkeys

Contact Info

Product

Resources

About

Use of a Solid Formulation ofBeauveria bassianafor Biocontrol of the Red Palm Weevil (Rhynchophorus ferrugineus) (Coleoptera: Dryophthoridae) Under Field Conditions in SE Spain^*