Use of aminoguanidine, a selective inducible nitric oxide synthase inhibitor, to evaluate the role of nitric oxide in periapical inflammation

Razavi, SeyedMohammad; Jahadi, Sanaz; Saatchi, Masoud

doi:10.2334/josnusd.53.225

Cited by 15 publications

(12 citation statements)

References 30 publications

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“…[28] Hama et al , explained the mechanisms regarding the role of NO in the progression of periapical inflammation. [29] Our research group, in the first of series of investigations on NO, demonstrated that AG administration significantly reduced the inflammatory intensity in periapical lesions of cats,[30] which was consistent with the findings of the present study. The results of the current investigation showed that AG as a selective inhibitor of iNOS can promote the healing of periapical region.…”

Section: Discussionsupporting

confidence: 91%

The use of aminoguanidine, a selective inducible nitric oxide synthase inhibitor, to evaluate the role of nitric oxide on periapical healing

Razavi¹,

Nejad²

2011

Dent Res J

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Background:Nitric oxide (NO) is one of the many chemical mediators involved in inflammatory processes. In addition to periapical inflammation, NO can have a role in periapical healing. The purpose of this study was to evaluate the effect of aminoguanidine (AG) as a selective inhibitor of inducible nitric oxide synthase (iNOS) on the degree of healing response of periapical lesions of the canine teeth of cats.Methods:In this interventional experimental study, the root canals of 48 cat canine teeth were infected with cat dental plaque and sealed. After induction of periapical lesions, root canal therapy (RCT) was performed. On the day of RCT phase, the cats were administered either AG (experimental group) or normal saline (control group), which was continued on a daily basis until the day of sacrifice. Four canine teeth in one cat served as negative and positive controls. The animals were sacrificed 6 weeks after RCT. The healing response of the periapical zones was analyzed histologically. The mean scores of healing for the two groups were compared using Mann–Whitney U test.Results:The mean scores of healing for the AG group (2.45±0.508) were significantly higher than those of the control group (2±0.510) (P<0.05).Conclusion:The use of an iNOS selective inhibitor such as AG can accelerate the healing process in periapical lesions.

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Section: Discussionsupporting

confidence: 91%

The use of aminoguanidine, a selective inducible nitric oxide synthase inhibitor, to evaluate the role of nitric oxide on periapical healing

Razavi¹,

Nejad²

2011

Dent Res J

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“…Histopathological changes were graded in a blinded manner as described previously (Farhad et al . ). The boundaries of the periapical lesions associated with the mesial roots were determined using the freehand drawing tool of the image analysis program, and the lesion area was calculated.…”

Section: Methodsmentioning

confidence: 97%

“…The histological parameters of the samples were examined using light microscopy (CX41; Olympus Corporation, Tokyo, Japan) and an image analysis program (Database Manual Cell Sens Life Science Imaging Software System; Olympus Corporation) by a physician specialized in Pathology. Histopathological changes were graded in a blinded manner as described previously (Farhad et al 2011). The boundaries of the periapical lesions associated with the mesial roots were determined using the freehand drawing tool of the image analysis program, and the lesion area was calculated.…”

Section: Histopathological Evaluationmentioning

confidence: 99%

Anti‐inflammatory and antiresorptive functions of melatonin on experimentally induced periapical lesions

Sarıtekin¹,

Kaya²,

Aşçı³

et al. 2019

Int Endodontic J

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Aim To investigate the effects of systemically administered melatonin on inflammation and alveolar bone resorption in rats with experimentally induced periapical lesions. Methodology Thirty adult Sprague Dawley rats were divided equally into negative, positive control and melatonin groups. The pulp chambers of their mandibular first molars were exposed to the oral environment to induce experimental periapical lesions in the positive control and melatonin groups. The melatonin group received daily intraperitoneal injections of melatonin at a dose of 10 mg kg−1. After 21 days, the animals were euthanized; the hemi‐mandible parts were prepared for radiological, histopathological, immunohistochemical (IL‐1β, RANK, RANKL, OPG and tartrate‐resistant acid phosphatase (TRAP) and Brown–Brenn (bacteria) evaluations. Data were analysed by Kruskal–Wallis (for non‐parametric data) and one‐way anova tests (for parametric data) (P < 0.05). Results The area of radiographic periapical bone loss was significantly smaller in rats that were given daily intraperitoneal injections of melatonin (P < 0.01). The histopathological scores of the melatonin group were significantly lower than those of positive control group (P < 0.01). Histomorphometrically, the area of periapical bone loss in the melatonin group was significantly smaller than the positive control group (P < 0.01). The expression of IL1‐β, RANK and RANKL was significantly higher in the positive control group, whereas OPG was significantly higher in the melatonin group (P < 0.01). The number of osteoclasts was significantly greater in the positive control group by TRAP staining analyses (P < 0.01). The scores for bacteria localization using Brown–Brenn staining in the melatonin group was significantly lower than that of the positive control group (P < 0.01). Conclusions Melatonin demonstrated antiresorptive effects on bone associated with experimentally induced periapical lesions in rats via its anti‐inflammatory activity. Further studies are necessary to evaluate its possible effects on the healing of periapical lesions.

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“…[ 35 ] Farhad et al . [ 14 ] in their study on a cat model evaluated the effect of systemic administration of AG on the severity of inflammation and demonstrated that AG significantly decreased the severity of inflammation in periapical lesions. In another study, Farhad et al .…”

Section: Discussionmentioning

confidence: 99%

“…[ 13 ] Farhad et al . [ 14 ] in their study on the role of NO in the progression of the inflammatory process in the periapical tissue in a cat model demonstrated that systemic administration of a selective iNOS inhibitor (AG) significantly decreased the severity of inflammation in the periapical lesions induced in the periapical region of canine teeth. Moreover, Farhad et al .…”

Section: Introductionmentioning

confidence: 99%

Selective nitric oxide synthase inhibitor promotes bone healing

et al. 2017

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Background:Nitric oxide (NO) has many functions in wound healing and bone metabolism. This study sought to assess the local effect of aminoguanidine (AG), a selective inducible NO synthase (iNOS) inhibitor, on the rate of bone healing.Materials and Methods:This experimental interventional study was conducted on 36 rats, which were randomly divided into three groups of control, placebo, and AG. Bone defects measuring 5 mm × 5 mm were created in the femur. In control group, bone defects remained empty. A placebo gel was applied to defects in the placebo group. AG gel was placed in bone defects in AG group. New bone formation and healing were assessed using histological and histomorphometric analyses. The healing score and the percentage of new bone formation (total bone mass, immature bone, and mature bone) were compared among the three groups using the Kruskal–Wallis test and analysis of variance, respectively. A P < 0.05 was statistically significant.Results:The mean healing score in AG group (3.17 ± 0.577) was significantly higher than that in control (2.67 ± 0.49) and the placebo (2.58 ± 0.515) groups (P = 0.036). The percentage of new mature (lamellar) bone in AG group (22.06 ± 1.90) was significantly higher than that in control (20.94 ± 2.03) and the placebo (20.53 ± 1.20) groups (P = 0.008).Conclusion:The rate of bone healing was faster in the AG compared to the other two groups. Local application of selective iNOS inhibitors like AG may be efficient as an adjunct in the clinical setting where local bone formation is required.

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Use of aminoguanidine, a selective inducible nitric oxide synthase inhibitor, to evaluate the role of nitric oxide in periapical inflammation

Cited by 15 publications

References 30 publications

The use of aminoguanidine, a selective inducible nitric oxide synthase inhibitor, to evaluate the role of nitric oxide on periapical healing

The use of aminoguanidine, a selective inducible nitric oxide synthase inhibitor, to evaluate the role of nitric oxide on periapical healing

Anti‐inflammatory and antiresorptive functions of melatonin on experimentally induced periapical lesions

Selective nitric oxide synthase inhibitor promotes bone healing

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