Use of an Acoustic Wave Device as a Liquid Chromatography Detector

Tom-Moy, M.; Doherty, Thomas P.; Baer, R.L.; Spira-Solomon, Darlene

doi:10.1021/bk-1995-0613.ch002

ACS Symposium Series

1996

DOI: 10.1021/bk-1995-0613.ch002

|View full text |Cite

Use of an Acoustic Wave Device as a Liquid Chromatography Detector

M. Tom-Moy

Thomas P. Doherty

R.L. Baer

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1998

2000

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…17 The coupling of label-free detection techniques to liquid chromatography applying a direct assay format is described in the literature for the quantification of specific proteins. Tom-Moy et al 18 demonstrated the use of a surface transverse wave (STW) device as HPLC detector for human IgG. Recently, Bracewell et al 19 coupled an optical biosensor to affinity chromatography to detect the breakthrough of antibody fragments through a column.…”

mentioning

confidence: 99%

Label-Free Biochemical Detection Coupled On-Line to Liquid Chromatography

Haake

Irth

et al. 2000

Anal. Chem.

View full text Add to dashboard Cite

The on-line coupling of a label-free optical biosensor to a HPLC system is described by combining the separation power of HPLC with the specificity of the biosensor system. A highly cross-reactive antibody against the pesticide isoproturon was used as model for affinity proteins. The binding strength of the antibody to the utilized pesticides was characterized with the biosensor, first. In the on-line coupling setup, the eluate of the HPLC was mixed continuously with the antibodies. The presence of antigens was detected by a reduction of the antibody binding to the transducer. This reduced binding was quantified by a differentiation of the sensor signal by applying a Savitzky-Golay algorithm. Limits of detection were found to be in the femtomole range without preconcentration, which is comparable to a study using fluorescence-based biochemical detection.

show abstract

mentioning

confidence: 99%

Label-Free Biochemical Detection Coupled On-Line to Liquid Chromatography

Haake

Irth

et al. 2000

Anal. Chem.

View full text Add to dashboard Cite

show abstract

Biosensors in Chemical Separations

Fishman

Greenwald

Zare

1998

Annu. Rev. Biophys. Biomol. Struct.

View full text Add to dashboard Cite

Identification of biomolecules in complex biological mixtures represents a major challenge in biomedical, environmental, and chemical research today. Chemical separations with traditional detection schemes such as absorption, fluorescence, refractive index, conductivity, and electrochemistry have been the standards for definitive identifications of many compounds. In many instances, however, the complexity of the biomixture exceeds the resolution capability of chemical separations. Biosensors based on molecular recognition can dramatically improve the selectivity of and provide biologically relevant information about the components. This review describes how coupling chemical separations with online biosensors solves challenging problems in sample analysis by identifying components that would not normally be detectable by either technique alone. This review also presents examples and principles of combining chemical separations with biosensor detection that uses living systems, whole cells, membrane receptors, enzymes, and immunosensors.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Use of an Acoustic Wave Device as a Liquid Chromatography Detector

Cited by 2 publications

References 0 publications

Label-Free Biochemical Detection Coupled On-Line to Liquid Chromatography

Label-Free Biochemical Detection Coupled On-Line to Liquid Chromatography

Biosensors in Chemical Separations

Contact Info

Product

Resources

About