Use of an Acrylic Dendron-containing Monomer in the Synthesis of a Macroporous Polymeric Material

Khimich, G. N.; Tennikova, Tatiana

doi:10.1007/s11167-005-0355-3

Cited by 5 publications

(1 citation statement)

References 23 publications

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“…The advantage in application of GMA−EDMA monolithic material is the possibility of direct formation of a porous structure in a wide range of pore sizes (0.1−10 μm). The experimental work on optimization of polymerization parameters, such as a choice of initiator and its concentration, reaction time, and variation of porogenic composition, has been recently carried out and described elsewhere …”

Section: Resultsmentioning

confidence: 99%

Macroporous Monolithic Layers as Efficient 3-D Microarrays for Quantitative Detection of Virus-like Particles

2007

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The main objective of the present paper was to test the recently developed new type of 3-D protein microarray system based on glycidyl methacrylate-co-ethylene glycol dimethacrylate (GMA-EDMA) monolithic material for efficient and fast virus detection. The large-size synthetic particles bearing adsorption-responsible biomolecules on their surface were used as a virus model. Two affinity pairs were chosen for present study. Model virus-like particles, close to the dimensions of human viruses, were developed by means of protein (one of affinity partners) covalent binding to the outer carboxylated surface of polymer latexes (polystyrene based, 80-nm diameter). Recently, it was shown that the adsorption of similar synthetic particles was defined by a protein covering the particle surface. The corresponding complement was immobilized on the surface of prepared by photoinitiated polymerization GMA-EDMA macroporous layers. The detection of a formed biocomplementary complex between protein-bearing latex particle and immobilized affinity partner was carried out by two different methods: (1) similar to an ELISA approach using horse radish peroxidase conjugated with monoclonal antibodies and (2) direct method using two markers. In parallel, the pairing of native proteins was also evaluated. The adsorption behavior of studied particles has been additionally investigated by affinity adsorption at static and dynamic (frontal elution) conditions using the same GMA-EDMA material shaped as a short monolithic column (CIM Disk, BIA Separations, Ljubljana, Slovenia). The results obtained for these virus-mimicking supramolecular structures can be further used for the construction of a rapid, highly sensitive, and highly specific test intended for precise diagnostics of some respiratory tract infection viruses.

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Section: Resultsmentioning

confidence: 99%

Macroporous Monolithic Layers as Efficient 3-D Microarrays for Quantitative Detection of Virus-like Particles

2007

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Preparation of methacrylate monoliths

Влах

Tennikova²

2007

J of Separation Science

151

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Rigid macroporous polymers developed in the early 1990s are widely used as efficient stationary phases for all types of chromatographic separations. The main advantages of so-called monolithic supports are their high hydraulic permeability and the dominance of the convection over the diffusion mechanism of mass-exchange under dynamic conditions that allow the separation to be carried out at extremely high flow rates and, consequently, during very short operation times. Among other types of macroporous polymers, the methacrylate-based monolithic materials represent the most popular and successfully explored class of sorbents. This review is an attempt to collect together the contributions of different groups working in the area of monolith preparation. Examples of different methcrylate monomers and crosslinkers, as well as porogenic solvents, including polymer ones, used in monolith preparation are discussed.

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