Use of an anthocyanin production phenotype as a visible selection marker system in transgenic tobacco plant

Lim, Sung-Hun; Sohn, Seong-Han; Kim, Donghern; Kim, Jae Kwang; Lee, Jong-Yeol; Kim, Youngmi; Ha, Sun-Hwa

doi:10.1007/s11816-012-0215-6

Cited by 23 publications

(29 citation statements)

References 30 publications

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“…The resultant vector (pB7WG2D-RsMYB1) was maintained in Agrobacterium strain LBA4404 for infiltration of the abaxial leaf surface of Nicotiana tabacum. After infiltration, the leaf color was monitored at 5 days post-infiltration (dpi), as described in Lim et al (2012). Construct pB7WG2D-RsMYB1 in Agrobacterium strain GV3101 was transformed into Arabidopsis (Columbia-0 genetic background) using the floral dipping method and the transformed Arabidopsis seeds were grown in soil under a 16 h light/8 h dark regimen at 20°C (Clough and Bent 1998).…”

Section: Transient Assay Of Rsmyb1 Function In Anthocyanin Productionmentioning

confidence: 99%

Activation of anthocyanin biosynthesis by expression of the radish R2R3-MYB transcription factor gene RsMYB1

et al. 2015

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RsMYB1, a MYB TF of red radish origin, was characterized as a positive regulator to transcriptionally activate the anthocyanin biosynthetic machinery by itself in Arabidopsis and tobacco plants. Anthocyanins, providing the bright red-orange to blue-violet colors, are flavonoid-derived pigments with strong antioxidant activity that have benefits for human health. We isolated RsMYB1, which encodes an R2R3-MYB transcription factor (TF), from red radish plants (Raphanus sativus L.) that accumulate high levels of anthocyanins. RsMYB1 shows higher expression in red radish than in common white radish, in both leaves and roots, at different growth stages. Consistent with RsMYB1 function as an anthocyanin-promoting TF, red radishes showed higher expression of all six anthocyanin biosynthetic and two anthocyanin regulatory genes. Transient expression of RsMYB1 in tobacco showed that RsMYB1 is a positive regulator of anthocyanin production with better efficiency than the basic helix-loop-helix (bHLH) TF gene B-Peru. Also, the synergistic effect of RsMYB1 with B-Peru was larger than the effect of the MYB TF gene mPAP1D with B-peru. Arabidopsis plants stably expressing RsMYB1 produced red pigmentation throughout the plant, accompanied by up-regulation of the six structural and two regulatory genes for anthocyanin production. This broad transcriptional activation of anthocyanin biosynthetic machinery in Arabidopsis included up-regulation of TRANSPARENT TESTA8, which encodes a bHLH TF. These results suggest that overexpression of RsMYB1 promotes anthocyanin production by triggering the expression of endogenous bHLH genes as potential binding partners for RsMYB1. In addition, RsMYB1-overexpressing Arabidopsis plants had a higher antioxidant capacity than did non-transgenic control plants. Taken together, RsMYB1 is an actively positive regulator for anthocyanins biosynthesis in radish plants and it might be one of the best targets for anthocyanin production by single gene manipulation being applicable in diverse plant species.

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Section: Transient Assay Of Rsmyb1 Function In Anthocyanin Productionmentioning

confidence: 99%

Activation of anthocyanin biosynthesis by expression of the radish R2R3-MYB transcription factor gene RsMYB1

et al. 2015

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“…The recombinant vector was transformed into Agrobacterium tumefaciens GV3101 for infiltration into Nicotiana tabacum leaves which were grown in the greenhouse at 25 °C and 16/8 h light/dark. Details of the infiltration were the same as those described by Lim et al (2012) [46]. In brief, Agrobacterium tumefaciens GV3101 with recombinant vector was grown at 28 °C, 220 rpm in LB medium including 50 μg/mL kanamycin, 10 mM MES, and 20 μM AS.…”

Section: Transient Assays Of Snmyb Functionmentioning

confidence: 99%

Identification of Anthocyanins Compositions and Functional Analysis of An Anthocyanin Activator in <em>Solanum nigrum</em> Fruits

Wang¹,

Zhang²,

Ren³

et al. 2017

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Solanum nigrum fruits have been conventionally available as a material of beverage due to its nutritional substances such as minerals, vitamins, amino acids, proteins, sugars, polyphenols and anthocyanins. Here has rarely reported on the characterizaton of the components and the regulatory mechanism of Anthocyanins in S. nigrum. In this study, we determined that the peel and flesh of S. nigrum fruits shared the similar HPLC profiles, but the different contents and total antioxidant activities for Anthocyanins. After an efficient purification method mainly including extraction with pH 1.0 distilled water and then desorption with pH 1.0 95% ethanol after a DM-130 resin adsorption step to obtain more pure anthocyanins extracts, the purity of anthocyanins extract from S. nigrum fruits reached to 56.1%. Moreover, eight anthocyanins from S. nigrum fruit were identified with HPLC-MS/MS for the first time. A typical R2R3-MYB transcription factor gene, SnMYB, was also cloned for the first time by RACE-PCR from S. nigrum. Moreover, the contents of anthocyanins was shown a good correlation (r = 0.93) with the expression levels of SnMYB during the fruits developmental stages. Most significantly, SnMYB successfully produced high anthocyanins contents (1.03 mg/g) when SnMYB was transiently expressed in tobacco leaves. Taken together, S. nigrum fruits are a promising resource for anthocyanins extraction and SnMYB is an activator that positively regulates anthocyanins biosynthesis in S. nigrum.

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“…The anthocyanin content (ACC) was evaluated using the pH diff erential method (Lim et al, 2012). The results were calculated as cyanidin-3-glucoside equivalents [CGE mg/100 g] of sample fresh weight (FW).…”

Section: Anthocyanin Contentsmentioning

confidence: 99%

The antioxidant and phenolic proﬁles of ﬁve green vegetables grown in Southern Nigeria

Adeosun

Ighodaro

Aminu

et al. 2016

Acta Sci Pol Technol Aliment

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Use of an anthocyanin production phenotype as a visible selection marker system in transgenic tobacco plant

Cited by 23 publications

References 30 publications

Activation of anthocyanin biosynthesis by expression of the radish R2R3-MYB transcription factor gene RsMYB1

Activation of anthocyanin biosynthesis by expression of the radish R2R3-MYB transcription factor gene RsMYB1

Identification of Anthocyanins Compositions and Functional Analysis of An Anthocyanin Activator in <em>Solanum nigrum</em> Fruits

The antioxidant and phenolic proﬁles of ﬁve green vegetables grown in Southern Nigeria

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Use of an anthocyanin production phenotype as a visible selection marker system in transgenic tobacco plant

Cited by 23 publications

References 30 publications

Activation of anthocyanin biosynthesis by expression of the radish R2R3-MYB transcription factor gene RsMYB1

Activation of anthocyanin biosynthesis by expression of the radish R2R3-MYB transcription factor gene RsMYB1

Identification of Anthocyanins Compositions and Functional Analysis of An Anthocyanin Activator in <em>Solanum nigrum</em> Fruits

The antioxidant and phenolic pro&#64257;les of &#64257;ve green vegetables grown in Southern Nigeria

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The antioxidant and phenolic proﬁles of ﬁve green vegetables grown in Southern Nigeria