Use of an Antiglobulin Serum to detect Cells producing Antibody with Low Haemolytic Efficiency

Dresser, D. W.; Wortis, D H

doi:10.1038/208859a0

Cited by 274 publications

(78 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Instead inhibition of 19S hemolysis of at least one dilution step was a common finding. This specific potentiating effect of the 7S hemolysis in indirect hemolysis using a rabbit-anti-mouse 7S antiserum is in accordance with previous findings (14,15).…”

Section: I~ir~t Hemolysis At the Cellular And Serum Level--the Inoculsupporting

confidence: 81%

“…The antisera used to develop 7S plaques in the indirect hemolytic plaque test (14,15) were produced in rabbits immunized with mouse 7S antibodies by the following technique. Mouse anti-human serum albumin (HSA) antibodies of 7S type were obtained after gel filtration on Sephadex G-200.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

“…The indirect hemolytic plaque test detecting 7S-producing cells (14)(15)(16) was carried out according to Dresser and Wortis (15). A high degree of inhibition of 19S plaques was found when using our unabsorbed rabbit-anti-mouse 7S antiserum confirming the findings of others (15). Using our unabsorbed antiserum in two experimental systems where only 19S antibody synthesis could be recorded the average inhibition of 19S plaques in 22 different tests was found to be 80.4% (st. = 4.1%) (20).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Antibody Synthesis at the Cellular Level

Wigzell

1966

The Journal of Experimental Medicine

View full text Add to dashboard Cite

Specific inhibition of the humoral component of the immune response by passively administered antibody has been demonstrated in a variety of experimental systems indicating the important role of antibody as a feedback factor during immune response (1-8, and earlier references quoted in reference 1). Both 19S and 7S antibodies have been found to function as inhibitors, 7S as a rule being more efficient than 19S antibodies (6-8). Passively transferred antibody has been found particularly efficient as an inhibitor if administered prior to or during the induction of both 19S and 7S antibody synthesis, whereas an already producing 7S system has been considered more resistant to inhibition than a comparable 19S system (6-8). This difference between the two antibody systems is in agreement with findings t h a t 7S synthesis in most systems is very long-lived compared to 19S production indicating either that the 7S-producing system is not as easily depleted of antigen as is the 19S system or the 7S synthesizing cells are very long-lived even in complete absence of antigen 9-12).In the present article the concept of the difference between 19S-and 7S-synthesizing systems with regard to sensitivity to antibody-induced suppression has been challenged. Precise studies of 19S and 7S antibody synthesis at a given moment are now possible at the cellular level using the direct (13) and indirect (14--16) hemolytic plaque tests. Using these techniques, particular attention has been focused on the effect of giving antibody well after the primary peaks of 19S and 7S synthesis. Material and MetkodsAnimals.--Mice of the following inbred strains were used: A/SnK1, A.BY/KI, A.SW/K1, CBA/KI, C3H/K1, and C57BL/K1. Some experiments were performed using Fl-hybrids of two of these strains. The mice were between 2 and 5 months of age when used. Within each experiment all mice were of the same strain, sex, and age.Angbodies.--Antibodies for inhibition were produced by repeated intraperitoneal injections of 4 X 108 sheep or chicken red blood cells (SRBC or ChRBC) into groups of 40 to 100 mice. The animals received on the average five to twelve 2-wk injections and were bled 5 to 10 days after the last one. Care was taken to ensure that the animals were immunized at least once against the same batch of erythrocytes that was used in the inhibition experiment(s) for induction of the primary response. The antisera for inhibition were produced and used within the same strain. 953on May 7, 2018 jem.rupress.org Downloaded from

show abstract

Section: I~ir~t Hemolysis At the Cellular And Serum Level--the Inoculsupporting

confidence: 81%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Antibody Synthesis at the Cellular Level

Wigzell

1966

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

“…Hemolytic plaques developed around the ceils synthesizing antibody of high hemolytic efficiency ("19S"). In certain experiments, plaques produced by antibody of lower hemolytic efficiency ("7S") (20,21), were developed by exposing duplicate plates to an appropriate dilution of a rabbit antiserum to whole mouse serum, for 1 hr prior to incubation of the plates with complement. The numbers of PFC per recipient spleens were calculated per 1 X 107 donor ceils injected.…”

Section: Methodsmentioning

confidence: 99%

Relationship of Germinal Centers in Lymphoid Tissue to Immunological Memory

Wakefield

Thorbecke

1968

The Journal of Experimental Medicine

View full text Add to dashboard Cite

Transfer of adoptive immunity has proved useful in detection and characterization of the ceils carrying immunological memory. By introducing varying time intervals between transfer and reexposure to antigen in the recipients, the duration of responsiveness of memory cells was defined. Perkins and Makinodan (1) showed that, with donor ceils taken 4--6 wk after sensitization, little change in responsiveness of the primed cells occurs over the first few days following transfer. The results of Dresser (2) and Celada (3), however, indicate that when challenge with antigen is delayed for a month or longer after transfer there is a progressive decay in the responsiveness of the transferred cells. Indeed, Celada has shown a biphasic dedine of memory upon transfer of sensitized cells, which he interpreted to be due to two populations of memory cells, having half-lives of 26 and 190 days, respectively.The importance of nondividing or slowly proliferating cells in the maintenance of long-term immunity to certain antigens has been illustrated in a number of test systems. Lack of incorporation of thymidine-*H by primed cells transferred at short intervals after challenge with antigen in vivo, led Cohen and Talmage (4) to condude that the memory cell was not part of a rapidly dividing cell population. This condusion is compatible with the more recent findings of Gowans and Uhr (5) that long-term memory for the immune response to $X174 can be transferred by thoracic duct cells consisting almost entirely of small lymphocytes. X-irradiation studies have also suggested that nonreplicating cells are involved in long-term immunological memory (6-8). Moreover, long-lived small lymphocytes from peripheral blood of sensitized individuals have been identified as the cell type capable of transforming into blasts upon reexposure to antigen (9).The precursors of these nondividing cells which must be present during the early stages of the development of immunological memory, and the stimuli needed for such precursors to differentiate into small lymphocytes, are of particular theoretical *

show abstract

Regulatory Mechanisms in Antibody Synthesis

Möller¹

1969

Novartis Foundation Symposia

View full text Add to dashboard Cite

Use of an Antiglobulin Serum to detect Cells producing Antibody with Low Haemolytic Efficiency

Cited by 274 publications

References 2 publications

Antibody Synthesis at the Cellular Level

Antibody Synthesis at the Cellular Level

Relationship of Germinal Centers in Lymphoid Tissue to Immunological Memory

Regulatory Mechanisms in Antibody Synthesis

Contact Info

Product

Resources

About