Use of an Immunoglobulin G Avidity Test To Discriminate between Primary and Secondary Dengue Virus Infections

Souza, Vanda Akico Ueda Fick de; Fernandes, Silvana; Araújo, Evaldo Stanislau Affonso de; Tateno, Adriana Fumie; Oliveira, Olímpia M. N. P. F.; Oliveira, Renato dos Reis; Pannuti, Cláudio Sérgio

doi:10.1128/jcm.42.4.1782-1784.2004

Cited by 83 publications

(70 citation statements)

References 32 publications

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“…The studies carried out by De Souza et al have shown for the first time that, by using a commercial kit for serological dengue diagnosis, it was possible to discriminate between a case of primary dengue infection and a case of secondary dengue infection by detecting avid IgG (8). Our results, obtained with a serological test developed in our laboratory, confirm those obtained by De Souza.…”

Section: Discussionsupporting

confidence: 81%

“…The method of choice seems to be the measurement of the antigen-binding avidity of specific IgG: a first study showed the usefulness of IgG avidity to discriminate between primary and secondary dengue infection (8). The avidity assay is based on the fact that the first antibodies synthesized after an antigenic challenge or primary infection have a lower affinity for the antigen than those produced later on.…”

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confidence: 99%

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Discrimination between Primary and Secondary Dengue Virus Infection by an Immunoglobulin G Avidity Test Using a Single Acute-Phase Serum Sample

et al. 2005

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For clinical and epidemiological purposes, it is necessary to be able to classify serological responses during dengue virus infection. Thus, it is important to develop a test that can distinguish between primary and secondary serological responses. The hemagglutination inhibition (HI) test, which is currently recommended by the World Health Organization, is complicated to perform. We developed an enzyme-linked immunosorbent assay based on changes in the avidity of immunoglobulin G during the infectious episode. This test can discriminate between primary and secondary infections by using a single serum sample collected during the acute phase of infection. We took 1,140 avidity measurements with 118 pairs of serum samples or sequential samples taken from patients classified as having primary or secondary infection according to World Health Organization laboratory criteria. The mean percent avidity was significantly lower during primary infection (25.9%) than during secondary infection (66.3%) (Student t test, P < 0.001). The test had a sensitivity of 82.7% (95% confidence interval [CI] ‫؍‬ 79.0 to 86.6) and a specificity of 77.5% (95% CI ‫؍‬ 73.3 to 81.7). Based on analysis of only blood samples collected between the third and seventh days of the illness, during which most clinical complications occur, the sensitivity and specificity of the test were 95.1% (95% CI ‫؍‬ 92.6 to 97.7) and 80.0% (95% CI ‫؍‬ 75.3 to 84.7), respectively. This rapid and simple test appears to be an excellent alternative to the HI test for discriminating between primary and secondary dengue virus infections during the acute phase of dengue.

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Section: Discussionsupporting

confidence: 81%

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confidence: 99%

Discrimination between Primary and Secondary Dengue Virus Infection by an Immunoglobulin G Avidity Test Using a Single Acute-Phase Serum Sample

et al. 2005

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“…Therefore, we next examined the average affinity of the HA-binding Abs by using a modified ELISA to dissociate plate-bound, low-affinity Abs selectively via treatment with 7 M urea (31). Remarkably, compared with IgG Abs at day 7, those at day 4 were more resistant to urea detachment after boosting with whole-virion vaccines (Fig.…”

Section: Alum-adjuvanted Whole-and Split-virion Vaccines Comparably Imentioning

confidence: 99%

Whole-Virion Influenza Vaccine Recalls an Early Burst of High-Affinity Memory B Cell Response through TLR Signaling

Onodera

Hosono

Odagiri

et al. 2016

The Journal of Immunology

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Inactivated influenza vaccines have two formulations, whole- and split-virion types; however, how differential formulations impact their booster effects remain unknown. In this study, we demonstrate that whole-virion vaccines recall two waves of Ab responses, early T cell–independent (TI) and late T cell–dependent responses, whereas split-virion vaccines elicit the late T cell–dependent response only. Notably, higher-affinity Abs with improved neutralizing activity are provided from the early TI response, which emphasizes the important contribution of the formulation-dependent response in the protective immunity. Moreover, we show that the early TI response completely requires B cell–intrinsic TLR7 signaling, which can be delivered through viral RNAs within whole-virion vaccine. Thus, our results indicate that TLR agonists in whole-virion type improve recall Ab responses by directly targeting memory B cells, a finding with important implications for vaccine strategies aimed at the prompt recall of high-affinity neutralizing Abs.

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“…Local antibody avidity was estimated by ELISA (urea test) as described by de Souza 20 . The method is based on the ability of urea (chaotropic agent) to dissociate antigen–antibody complexes with weak avidity.…”

Section: Methodsmentioning

confidence: 99%

B‐ and T‐cell memory elicited by a seasonal live attenuated reassortant influenza vaccine: assessment of local antibody avidity and virus‐specific memory T‐cells using trogocytosis‐based method

Petukhova

Korenkov

Chirkova

et al. 2011

Influenza Resp Viruses

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Please cite this paper as: Petukhova et al. (2011) B‐ and T‐cell memory elicited by a seasonal live attenuated reassortant influenza vaccine: assessment of local antibody avidity and virus‐specific memory T‐cells using trogocytosis‐based method. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750‐2659.2011.00279.x. Purpose The main purpose of vaccination is to generate immunological memory providing enhanced immune responses against infectious pathogens. The standard and most commonly used assay for influenza vaccine immunogenicity evaluation is a hemagglutination inhibition assay (HAI). It is clear now that HAI assay is unable to properly assess the proven protective immunity elicited by live attenuated influenza vaccines (LAIV). New methods need to be developed for more accurate LAIV immunogenicity assessment and prediction of vaccine efficacy among target populations. Objective Randomized placebo‐controlled study of memory B‐ and T‐cell responses to intranasal LAIV in young adults. Methods A total of 56 healthy young adults 18–20 years old received seasonal monovalent LAIV. Mucosal memory B‐cell responses were measured by IgA avidity assessment in nasal swabs. CD4 memory T cells in peripheral blood were examined by the expression of CD45RO marker and in functional test by the ability of virus‐specific T cells to maintain the trogocytosis with antigen‐loaded target cells. Results Intranasal LAIV immunization enhances mucosal IgA avidity even without reliable increases in antibody titers. At the day 21 after vaccination, up to 40% of subjects demonstrated significant increases in both total and virus‐specific CD4 memory T cells that were observed regardless of seroconversion rate measured by HAI assay. Conclusion The data suggest that immunogenicity of LAIV vaccines should be evaluated on the mucosal and cellular immunity basis. The assays applied could be used to support influenza clinical trials through preliminary screening of volunteers and subsequent measurement of anti‐influenza in immunity.

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Use of an Immunoglobulin G Avidity Test To Discriminate between Primary and Secondary Dengue Virus Infections

Cited by 83 publications

References 32 publications

Discrimination between Primary and Secondary Dengue Virus Infection by an Immunoglobulin G Avidity Test Using a Single Acute-Phase Serum Sample

Discrimination between Primary and Secondary Dengue Virus Infection by an Immunoglobulin G Avidity Test Using a Single Acute-Phase Serum Sample

Whole-Virion Influenza Vaccine Recalls an Early Burst of High-Affinity Memory B Cell Response through TLR Signaling

B‐ and T‐cell memory elicited by a seasonal live attenuated reassortant influenza vaccine: assessment of local antibody avidity and virus‐specific memory T‐cells using trogocytosis‐based method

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