Use of an improved zirconyl hematoxylin stain in the diagnosis of Barrett's esophagus

McNulty, Jeannette M.; Kambour, M; Smith, Allen

doi:10.1111/j.1582-4934.2004.tb00327.x

Cited by 13 publications

(6 citation statements)

References 23 publications

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“…The sequence of dilutions was 1:10000, 1:5000, 1:2000, 1:1000, 1:500, 1:200. When a good concentration of primary antibody was found for the experimental section, of mouse antibody to epithelial membrane antigen (Vector Labs; clone GP1.4) and stained with aminoethylcarbazole (AEC) and counterstained with hematoxylin or stained with Vector ' s ImmPACT VIP ® and counterstained with 0.05% methylene green in 2.4% boric acid (McNulty et al 2004). Matching sections were incubated with 1:1000 antibody and stained with AEC or ImmPACT VIP ® and methylene green.…”

Section: Methodsmentioning

confidence: 99%

Finding the best antibody dilution by repeated immunostaining of the same tissue section

Smith

2013

Biotechnic & Histochemistry

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Section: Methodsmentioning

confidence: 99%

Finding the best antibody dilution by repeated immunostaining of the same tissue section

Smith

2013

Biotechnic & Histochemistry

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“…A methylene green counterstain for nuclei (McNulty et al 2004) is a 0.5% (w/v) solution of the dye in 2.4% aqueous boric acid. After 5 min in this solution, the slides are washed in two 2-min changes of H 2 O followed by three 3-min changes of 100% ethanol, clearing in xylene, and application of a resinous mounting medium.…”

Section: Ethyl Green (Ci 42590) and Methylene Green (Ci 52020)mentioning

confidence: 99%

Nuclear Stains

Kiernan¹

2008

Cold Spring Harb Protoc

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INTRODUCTIONFor the study of microscopic anatomy and of pathological material, it is usual to stain sections of tissue in such a way as to impart a dark color to the nuclei of cells and a lighter, contrasting color to the cytoplasm and extracellular structures. Nuclear stains, including cationic, anionic, and metal complexing dyes, are considered in this article. The rationales of the techniques are discussed, but methods of higher chemical specificity (e.g., for nucleic acids, carbohydrates, and functional groups of proteins) are not covered.

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“…The significance of microscopic foci of SIM at the GEJ, corresponding either to so‐called ultrashort segment CLE, or to carditis with intestinal metaplasia, is still a matter of debate 11 . The use of an improved zirconyl hematoxylin stains in the diagnosis of SIM has been reported recently 12 . A novel monoclonal antibody (MAbDAS‐1) that specifically reacts with colonic, but not small intestinal, epithelium recognizes esophageal SIM.…”

Section: Are There Molecular Markers For the Diagnosis Of Sim?mentioning

confidence: 99%

“…11 The use of an improved zirconyl hematoxylin stains in the diagnosis of SIM has been reported recently. 12 A novel monoclonal antibody (MAbDAS-1) that specifically reacts with colonic, but not small intestinal, epithelium recognizes esophageal SIM. None of the squamous epithelium and cardia specimens reacted with MAbDAS-1.…”

Section: What Is the Definition Of Columnar Lined Esophagus?mentioning

confidence: 99%

Columnar lined (Barrett's) esophagus: Future perspectives

Bani-Hani

2007

J of Gastro and Hepatol

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Columnar lined esophagus (CLE) or Barrett's esophagus is the precursor for esophageal adenocarcinoma. Future advances in understanding and management of this condition as well as improving the quality of care of CLE patients depends on answering essential questions. It is important to standardize the criteria for CLE definition. The rapid increase in incidence of CLE and adenocarcinoma raises serious concerns that the current management of gastroesophageal reflux disease (GERD) needs reassessment. The risk factors that determine who will and will not develop CLE are as yet undetermined. There is a need to develop a clinical risk stratification tool, which will help in determining who should be screened. The impact of elimination of GERD on the natural history of CLE is one important area for future research. The benefit of surveillance strategies remains unproven and the ideal endoscopic frequency, protocols and markers of cancer risk are unknown. Dysplasia may not provide the gold standard marker of cancer risk because of some inherited problems. A better technique than the current endoscopic pinch biopsy protocol is needed. To overcome the limitations of histological markers, many other markers of cancer risk needs to be developed and validated. The key question as to whether cancer risk is actually reduced by the new ablation modalities remains unanswered. The natural history of dysplasia and its management needs to be clarified. Although many questions have to be answered, it seems, however, that at least some answers exist, and these and proposals for answering some of these questions are underlined throughout this review.

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Use of an improved zirconyl hematoxylin stain in the diagnosis of Barrett's esophagus

Cited by 13 publications

References 23 publications

Finding the best antibody dilution by repeated immunostaining of the same tissue section

Finding the best antibody dilution by repeated immunostaining of the same tissue section

Nuclear Stains

Columnar lined (Barrett's) esophagus: Future perspectives

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