Use of an in vitro model in tissue engineering to study wound repair and differentiation of blastema tissue from rabbit pinna

Abstract

Rabbit ear wound repair is an accepted model for studies of tissue regeneration, leading to scar less wound repair. It is believed that a specific tissue, blastema, is responsible for such interesting capacity of tissue regeneration. To test this idea further and to elucidate the cellular events happening during the ear wound repair, we designed some controlled experiments in vitro. Small pieces of the ear were punched and washed immediately with normal saline. The tissues were then cultured in the Dulbecco's Modified Eagle(')s Medium, supplemented with fetal bovine serum in control group. As a treatment vitamin A and C was used to evaluate the differentiation potency of the tissue. These tissues were fixed, sectioned, stained, and microscopically studied. Micrographs of electron microscopy provided evidences revealing dedifferentiation of certain cells inside the punched tissues after incubation in tissue culture medium. The histological studies revealed that cells of the tissue (i) can undergo cellular proliferation, (ii) differentiate to epithelial, condrogenic, and osteogenic tissues, and (iii) regenerate the wounds. These results could be used for interpretation of the possible events happening during tissue engineering and wound repair in vitro. An important goal of this study is to create a tissue engineering and tissue banking model, so that in the future it could be used in further blastema tissue studies at different levels.

The in vitro analysis of migration and polarity of blastema cells in the extracellular matrix derived from bovine mesenteric in the presence of fibronectin

“…Therefore, the 3D cell culture in vitro can provide a similar cell culture environment to animal models [ 25 , 26 ]. After punching the rabbit pinna, the growth of the epithelial tissue is converged on repairing the hole in vivo [ 13 , 27 ]. In this study, a decellularized mesenteric matrix in the presence of FN was used to evaluate the interaction with blastema tissue.…”

“…Also, the evaluation of cell migration and dynamic tissue culture could pave the way to understand molecular and cellular mechanisms underlying tissue repair in multicellular organisms. Blastema tissue is one of the types of tissues created during the repair process [ 12 , 13 ]. It contains a group of undifferentiated cells possessing the ability to proliferate, differentiate, and migrate [ 11 - 13 ].…”

“…Blastema tissue is one of the types of tissues created during the repair process [ 12 , 13 ]. It contains a group of undifferentiated cells possessing the ability to proliferate, differentiate, and migrate [ 11 - 13 ]. Previous studies have shown that organisms that develop blastema tissue could be attractive experimental living models to investigate the process of differentiation, development, and repair.…”

Uncovering the regeneration strategies of zebrafish organs: a comprehensive systems biology study on heart, cerebellum, fin, and retina regeneration

“…The fourth step is the differentiation of recalled-blastema-like formation and pattern formation step. The model whereby blastema tissue differentiates into epithelial, chondrogenic, and osteogenic tissues is highly regarded in studies of wound repair [ 34 ]. Pattern formation is the reproducible generation of complex and self-regulating patterns, where Wnt signaling was proposed to play a dual role: as an activator during the process, and as an inhibitor after the process [ 35 ].…”

Overexpression of Chemokine Receptors on Neural Stem Cells Pretreated with Valproic acid: Towards Improved Homing

“…These cells were digested by trypsin to produce single www.genesandcells.com/journal cells. Then, they were seeded immediately into T25 cell culture flasks at a concentration of 5000 cells/cm 2 (~200,000 cells/ml), containing Dulbecco's Modified Eagle's Medium-High Glucose (DMEM-HG, Gibco) supplemented with 15% fetal bovine serum (FBS, Gibco) [24]. These flasks were incubated for 48 hours in a 5% CO2 incubator at 37° C. After this period, the cell adhered to a new flask and floating tissue and cells are removed.…”