Use of an in vitro digestion method to evaluate the bioaccessibility of arsenic in edible seaweed by inductively coupled plasma-mass spectrometry

García-Sartal, Cristina; Romarís–Hortas, Vanessa; Barciela‐Alonso, María Carmen; Moreda-Piñeiro, Antonio; Domínguez-González, Raquel; Bermejo-Barrera, Pilar

doi:10.1016/j.microc.2010.12.001

Cited by 47 publications

(20 citation statements)

References 19 publications

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“…In vitro digestion method The in vitro digestion of seaweed was performed using a procedure recently reported elsewhere [23]. A 0.5 g of powdered seaweed was accurately weighed into 100-mL Erlenmeyer flask.…”

Section: Methodsmentioning

confidence: 99%

Two-dimensional HPLC coupled to ICP-MS and electrospray ionisation (ESI)-MS/MS for investigating the bioavailability in vitro of arsenic species from edible seaweed

García-Sartal

Taebunpakul

Stokes³

et al. 2011

Anal Bioanal Chem

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Edible seaweed consumption is a route of exposure to arsenic. However, little attention has been paid to estimate the bioaccessibility and/or bioavailability of arsenosugars in edible seaweed and their possible degradation products during gastrointestinal digestion. This work presents first use of combined inductively coupled plasma mass spectroscopy (ICP-MS) with electrospray ionization tandem mass spectrometry (ESI-MS/MS) with two-dimensional HPLC (size exclusion followed by anion exchange) to compare the qualitative and quantitative arsenosugars speciation of different edible seaweed with that of their bioavailable fraction as obtained using an in vitro gastrointestinal digestion procedure. Optimal extraction conditions for As species from four seaweed namely kombu, wakame, nori and sea lettuce were selected as a compromise between As extraction efficiency and preservation of compound identity. For most investigated samples, the use of ammonium acetate buffer as extractant and 1 h sonication in a water bath followed by HPLC-ICP-MS resulted in 40-61% of the total As to be found in the buffered aqueous extract, of which 86-110% was present as arsenosugars (glycerol sugar, phosphate sugar and sulfonate sugar for wakame and kombu and glycerol sugar and phosphate sugar for nori). The exception was sea lettuce, for which the arsenosugar fraction (glycerol sugar, phosphate sugar) only comprised 44% of the total extracted As. Interestingly, the ratio of arsenobetaine and dimethylarsinic acid to arsenosugars in sea lettuce extracts seemed higher than that for the rest of investigated samples. After in vitro gastrointestinal digestion, approximately 11-16% of the total As in the solid sample was found in the dialyzates with arsenosugars comprising 93-120% and 41% of the dialyzable As fraction for kombu, wakame, nori and sea lettuce, respectively. Moreover, the relative As species distribution in seaweed-buffered extracts and dialyzates was found to be very similar. Collection of specific fractions from the size-exclusion column to be analysed using anion-exchange HPLC-ESI-MS/MS enabled improved chromatographic selectivity, particularly for the less retained arsenosugar (glycerol sugar), facilitating confirmation of the presence of arsenosugars in seaweed extracts and dialyzates. Using this approach, the presence of arsenobetaine in sea lettuce samples was also confirmed.

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Section: Methodsmentioning

confidence: 99%

Two-dimensional HPLC coupled to ICP-MS and electrospray ionisation (ESI)-MS/MS for investigating the bioavailability in vitro of arsenic species from edible seaweed

García-Sartal

Taebunpakul

Stokes³

et al. 2011

Anal Bioanal Chem

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“…In a previous work published by our team [15], total arsenic concentrations in these raw seaweeds were calculated. We have estimated a total arsenic concentration of 79.1 ± 0.8 μg g −1 for raw Kombu, 53.8 ± 2.0 μg g −1 for raw Wakame, 43.7 ± 0.8 μg g −1 for raw Nori and 5.3 ± 0.05 μg g −1 for raw Sea Lettuce.…”

Section: Total Arsenic Determination In Samples By Icp-msmentioning

confidence: 99%

Effect of the cooking procedure on the arsenic speciation in the bioavailable (dialyzable) fraction from seaweed

Sartal

Barciela‐Alonso

Bermejo-Barrera

2012

Microchemical Journal

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“…In this case, the two first discriminant functions (DF1 and DF2) explained 97.5% (conditions I) and 97.9% (conditions II) of total variance. With respect to their classification models, LDA differentiated and correctly classified 100% of analyzed teas (20).…”

Section: Discussionmentioning

confidence: 99%

“…Composition of SGJs and SIJs was selected based on protocols reported for various food products [8,13,14,20,21]. Because tea is consumed as a fluid and rapidly passes from an oral cavity to a stomach, a mechanical process of chewing was ignored.…”

Section: In Vitro Gid Proceduresmentioning

confidence: 99%

“…Bioaccessibility of different metals has been estimated so far in breads [4], fruits and vegetables [4][5][6][7], fruit juices [8,9], coffees [10][11][12], milks [13,14], herbal remedies [15], infant formulas [13,16], meat [17], linseed, sesame and cereals [16,18], and edible seaweeds [19][20][21]. Unfortunately, just few papers were devoted so far to bioavailability/bioaccessibility of selected metals from tea infusions.…”

Section: Introductionmentioning

confidence: 99%

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Simplified Method of Multi-Elemental Analysis of Dialyzable Fraction of Tea Infusions by FAAS and ICP OES

Szymczycha‐Madeja

Wełna

Pohl

2019

Biol Trace Elem Res

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A fast and straightforward sample preparation procedure of the dialyzable fraction of infusions of teas prior to their analysis on Al, Ba, Ca, Cu, Fe, Mg, Mn, Ni, Sr, and Zn contents by flame atomic absorption spectrometry (FAAS) and inductively coupled plasma optical emission spectrometry (ICP OES) was developed and validated. The proposed methodology was based on acidification with HNO 3 only and demonstrated good analytical performance, i.e., precision (0.80-5.0%), accuracy (< 5%), recoveries of elements (97.4-105%), and their detection limits (0.075-1.1 μg L −1 ) along with linearity of calibration curves in the whole studied concentration ranges. Applicability of the evaluated procedure, being a useful alternative to time-consuming wet digestions, was tested by determining bioaccessibility of elements in 20 infusions of black (BT) and green (GT) teas as assessed with the aid of in vitro gastrointestinal digestion. Average contributions of bioaccessible fractions (%) of studied metals were as follows: 1.18 (Al)-40.7% (Ca) and 4.65% (Al)-46.3% (Ca) for BTs and GTs, respectively. Drinking daily four cups (1 L) of tea, recommended dietary intakes (RDIs) of Ca, Cu, Fe, Mg, and Zn were covered to a small degree (< 1.5%). Only bioaccessibility of Mn highly contributed to RDI for this metal. According to provisional tolerable weekly intakes (PTWIs) for toxic elements such as Al and Ni, consumption of both types of teas should not represent any health risk. Additionally, analysis of variance of results clearly indicated that BTs and GTs were mostly differentiated due to concentrations of the bioaccessible fraction of Al, Ba, Cu, and Ni.

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Use of an in vitro digestion method to evaluate the bioaccessibility of arsenic in edible seaweed by inductively coupled plasma-mass spectrometry

Cited by 47 publications

References 19 publications

Two-dimensional HPLC coupled to ICP-MS and electrospray ionisation (ESI)-MS/MS for investigating the bioavailability in vitro of arsenic species from edible seaweed

Two-dimensional HPLC coupled to ICP-MS and electrospray ionisation (ESI)-MS/MS for investigating the bioavailability in vitro of arsenic species from edible seaweed

Effect of the cooking procedure on the arsenic speciation in the bioavailable (dialyzable) fraction from seaweed

Simplified Method of Multi-Elemental Analysis of Dialyzable Fraction of Tea Infusions by FAAS and ICP OES

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