Use of Anterior Lobe of Prostate Gland in the Assay of Metakentrin

Greep, Roy O.; Dyke, H. B. Van; Chow, Bacon F.

doi:10.3181/00379727-46-12092

Cited by 95 publications

(23 citation statements)

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“…Several samples were simultaneously bioassayed and immunoassayed in terms of the same human standard. Bioassays for HLH were done by means of the ventral prostate weight in hypophysectomized immature male rats (11). It is important to note that a human LH standard is required for the purposes of radioimmunoassay because LH of other species reacts poorly or not at all in the assay.…”

Section: Methodsmentioning

confidence: 99%

Radioimmunoassay for Luteinizing Hormone in Human plasma Or Serum: Physiological Studies*

Odell¹,

Ross²,

Rayford³

1967

J. Clin. Invest.

501

104

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Summary. It is not practical to quantitate gonadotropin in the blood of normal men and women by utilizing bioassays. We have developed a method for sensitive, precise, and specific radioimmunoassay of luteinizing hormone (LH) in human serum or plasma. Antisera were developed against human chorionic gonadotropin, and one of these was selected for extensive cross-reaction with human LH. Highly purified LH was radioiodinated by the method of Greenwood, Hunter, and Glover. Separation of antibody-bound from free LH-131I was accomplished by a double antibody technique. Dose-response curves for the purified LH, an impure urinary LH preparation, pituitary powder, and LH in plasma were all identical. Immunoassay and bioassay of impure urinary and pituitary gonadotropin preparations in terms of a common standard resulted in an index of discrimination of close to unity. LH levels in plasma from 32 adult men and 30 women outside the midcycle ranged from 0.6 to 3.2 mfAg per ml (1 m/Ag of our laboratory LH standard is equivalent to 8 mU of the Second International Reference Preparation of Human Menopausal Gonadotropin). Levels were remarkably constant in men from day to day and in women except at midcycle, when a sharp peak occurred lasting less than 24 hours. In all women studied who had a midcycle LH peak, mean plasma LH levels during the follicular phase of the menstrual cycle were higher than mean values obtained during the luteal phase. Prepubertal children had detectable plasma LH, and mean values were only slightly less than in adults. Plasma from castrate men or women or postmenopausal women contained 4.5 to 10.5 mug per ml. Clomiphene treatment of four men resulted in a doubling of plasma LH in 5 days. Introduction Wide, Roos, and Gemzell (1), using an immunoassay based on hemagglutination inhibition, first reported that human luteinizing hormone (HLH) cross-reacts with human chorionic gonadotropin (HCG) for binding to antibodies directed against HCG. In 1964 Paul and Odell (2) first developed a radioimmunoassay for HCG.

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Section: Methodsmentioning

confidence: 99%

Radioimmunoassay for Luteinizing Hormone in Human plasma Or Serum: Physiological Studies*

Odell¹,

Ross²,

Rayford³

1967

J. Clin. Invest.

501

104

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“…The strains of rat to be used for the SVW assays described in these three pharmacopoeias are not specified.) The earlier generation of LH assay in which weight gain of male accessory organs was used as response, namely the ventral prostate weight gain assay (Greep et al 1941), used 21-22-day-old hypophysectomized rats of unspecified strain, in which the maturity and selectivity of LH target cells may have been more precisely defined. This finding of inter-laboratory differences in the specificities of SVW assays is of some significance because, as indicated above, this assay method has been generally adopted by Pharmacopoeias for the control of the LH content of therapeutic products.…”

Section: Selectivity Of Svw Assaysmentioning

confidence: 99%

The fourth International Standard for Human Urinary FSH and LH: specificities of LH seminal vesicle weight gain assays in the collaborative study differ between laboratories

Storring

Das²

2001

Journal of Endocrinology

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The fourth International Standard for Human Urinary FSH and LH (IS; in ampoules coded 98/704) was compared with the third International Standard for Urinary FSH and LH (IS 71/264) by 10 laboratories in nine countries, using FSH and LH in vivo bioassays.Estimates of the FSH content of the IS by augmented ovarian weight gain assays were homogeneous within each laboratory and over all laboratories. The combined weighted geometric mean estimate of FSH content of the IS (with 95% fiducial limits) in terms of IS 71/264 was 71·9 (69·0-74·9) IU/ampoule.Although estimates by seminal vesicle weight gain (SVW) assays of the relative LH activities of the IS and IS 71/264 were homogeneous within laboratories, estimates were heterogeneous between laboratories. This indicated differences between the spectrum of LH isoforms in the IS and IS 71/264, which were obtained from different manufacturers, and differences between the specificities of SVW assays performed in different laboratories. The differences between the specificities of SVW assays appeared to be related to interactions among mean laboratory seminal vesicle weights, age and genetic strain of rat. The finding of inter-laboratory differences in the specificities of SVW assays is of some significance, as this assay method has been generally adopted by Pharmacopoeias for the control of the LH content of therapeutic products. The combined unweighted geometric mean estimate of LH content of the IS (with 95% fiducial limits) in terms of IS 71/264 by SVW and ovarian ascorbate depletion assays was 70·2 (61·7-80·0) IU/ampoule.Estimates of the FSH and LH content of ampoules of the IS kept at increased temperatures suggested that the IS would be adequately stable under normal storage conditions.On the basis of these results, the World Health Organization Expert Committee on Biological Standardization established the preparation in ampoules coded 98/704 as the fourth International Standard for Human Urinary FSH and LH, and assigned to the contents of each ampoule an activity of 72 International Units of urinary FSH and an activity of 70 International Units of urinary LH.

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“…5). It should be noted that the numbering of a-subunit glycosylation sites used in this Review is that of bovine-a with gaps introduced in the human-a sequence at residues [5][6][7][8][9] (Fig. 1). (The glycosylation sites of the latter are actually at its Asn residues 52 and 78).…”

Section: Detailed Site-specific Studies Of Carbohydrate Structuresmentioning

confidence: 99%

“…Mammalian lutropin activity can be measured by its effectiveness in increasing the weight of the ventral lobe of the prostate gland of hypophysectomized rats [5] or in depleting ovarian ascorbic acid in pseudopregnant intact female rats [6]. Follitropin assays are based on its effectiveness in increasing ovarian weight in immature intact female rats [7] or mice [8] pretreated with excess human choriogonadotropin.…”

Section: Measurement Of Hormone Activitymentioning

confidence: 99%

Molecular structures of glycoprotein hormones and functions of their carbohydrate components

Hartree

Renwick

1992

Biochemical Journal

146

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Use of Anterior Lobe of Prostate Gland in the Assay of Metakentrin

Cited by 95 publications

References 0 publications

Radioimmunoassay for Luteinizing Hormone in Human plasma Or Serum: Physiological Studies*

Radioimmunoassay for Luteinizing Hormone in Human plasma Or Serum: Physiological Studies*

The fourth International Standard for Human Urinary FSH and LH: specificities of LH seminal vesicle weight gain assays in the collaborative study differ between laboratories

Molecular structures of glycoprotein hormones and functions of their carbohydrate components

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